“…[144] Three Mannich bases 148 (Figure 18) having a fluoroquinolone antibiotic as amine moiety introduced through aminomethylation of the triazolthione substrate (X = S) were shown to inhibit the growth of PC-3 prostate cancer cell line by 46 % to 56 % at 10 μM. [145] In a series of papers, the antitumor activity of Mannich bases 149, 150 and 151 (Figure 18) against two models of grafted murine tumors (Ehrlich ascites carcinoma and S-37) has been investigated, [146][147][148] and their ability to inhibit methylation of DNA in tumors was determined to be relatively poor to modest. [146,147] Out of these Mannich bases, compound 150 (R = C 6 H 5 , n = 1) exhibited 54 % tumor growth inhibition of S-37 at 155 mg/kg, and increased the lifespan of mice with Ehrlich ascites carcinoma by 35 %.…”