Thiophene-derived compounds are of interest in chemistry, biology and pharmacology because of their various pharmacological activities This study aims to investigate the antitumor and antibacterial activity of thiophene-2,5-dicarboxylic acid bis-aryl (alkyl) amide compounds. In this study, the properties of antibacterial and antitumor were determined in vitro media, for all compounds. As a result, cell viability experiments show that some of this compounds have effective in vitro antitumor activity. In addition, it has also been found that some compounds have an effective antibacterial activity.