Synthesis and anticoagulant activity of sodium alginate sulfates

Fan, Lihong; Jiang, Lan; Xu, Yongmei; Zhou, Yue; Yuan, Songliu; Xie, Weiguo; Long, Zhong-Heng; Zhou, Jinping

doi:10.1016/j.carbpol.2010.10.038

Cited by 97 publications

(62 citation statements)

References 20 publications

(10 reference statements)

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“…28,43 In particular to our knowledge, a pure alginate sulfate hydrogel was not previously reported probably due to the sulfation method used. 27 Different sulfating agents including sulfuric acid-carbodiimide, 27 chlorosulfonic acid-formamide, 29 and various SO 3 /complexes 44,45 have been used in the past to prepare sulfated alginate. Recently, we could show that SO 3 / pyridine is a mild sulfating agent for GAGs such as hyaluronan allowing proper control of the degree of sulfation, especially if a homogeneous sulfate group distribution and rather low DS S values are desired.…”

Section: Discussionmentioning

confidence: 99%

Chondrocyte Culture in Three Dimensional Alginate Sulfate Hydrogels Promotes Proliferation While Maintaining Expression of Chondrogenic Markers

Mhanna

Kashyap

Palazzolo

et al. 2014

Tissue Engineering Part A

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The loss of expression of chondrogenic markers during monolayer expansion remains a stumbling block for cell-based treatment of cartilage lesions. Here, we introduce sulfated alginate hydrogels as a cartilage biomimetic biomaterial that induces cell proliferation while maintaining the chondrogenic phenotype of encapsulated chondrocytes. Hydroxyl groups of alginate were converted to sulfates by incubation with sulfur trioxidepyridine complex (SO 3 /pyridine), yielding a sulfated material cross-linkable with calcium chloride. Passage 3 bovine chondrocytes were encapsulated in alginate and alginate sulfate hydrogels for up to 35 days. Cell proliferation was five-fold higher in alginate sulfate compared with alginate ( p = 0.038). Blocking beta1 integrins in chondrocytes within alginate sulfate hydrogels significantly inhibited proliferation ( p = 0.002). Sulfated alginate increased the RhoA activity of chondrocytes compared with unmodified alginate, an increase that was blocked by b1 blocking antibodies ( p = 0.017). Expression and synthesis of type II collagen, type I collagen, and proteoglycan was not significantly affected by the encapsulation material evidenced by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry. Alginate sulfate constructs showed an opaque appearance in culture, whereas the unmodified alginate samples remained translucent. In conclusion, alginate sulfate provides a three dimensional microenvironment that promotes both chondrocyte proliferation and maintenance of the chondrogenic phenotype and represents an important advance for chondrocyte-based cartilage repair therapies providing a material in which cell expansion can be done in situ.

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Section: Discussionmentioning

confidence: 99%

Chondrocyte Culture in Three Dimensional Alginate Sulfate Hydrogels Promotes Proliferation While Maintaining Expression of Chondrogenic Markers

Mhanna

Kashyap

Palazzolo

et al. 2014

Tissue Engineering Part A

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show abstract

“…Then the sodium nitrite solution was dropped into the sodium bisulfite solution while stirring at 90 • C and reacted for 1.5 h. In this way, the sulfating agent (N(SO 3 Na) 3 ) was obtained. The reaction was as follow [12,13] …”

Section: Preparation Of Sulfating Agent (N(so 3 Na) 3 )mentioning

confidence: 99%

Preparation and anticoagulant activity of N-succinyl chitosan sulfates

Wang

Zhou

Xie

et al. 2012

International Journal of Biological Macromolecules

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“…Thus, increase of the reaction temperature will promote the reaction. In contrast, since the sulfating reaction is an exothermic reaction, the high temperature hampers absorption of the sulfating agent by the quaternary ammonium chitosan, which result in a backward shift of the chemical equilibrium and lower the DS [28]. As shown in Fig.…”

Section: Influence Of the Reaction Temperature On The Dsmentioning

confidence: 81%

“…This may be attributed to the negatively charge of sulfate groups of quaternary ammonium chitosan sulfates to neutralize the positively charged amino acid residues which improve the anticoagulant activity in the antithrombin. The high DS can increase the density of negatively to inhibit the activity of antithrombin [28].…”

Section: Anticoagulant Activitymentioning

confidence: 98%