Synthese von Geranyl‐ und Farnesyl‐pyrophosphat

Comparison between Biosynthesis of ent-Kaurene in Germinating Tomato Seeds and Cell Suspension Cultures of Tomato and Tobacco

“…The edges of the paper were dipped in water and the seeds were germinated at 24 C. Water was changed every day and seeds were harvested after three days. (4), as reported by Popjak et al (16). The amounts of reagents used for pyrophosphorylation, the duration of the reaction, and the final purification of the pyrophosphate esters were done according to the procedure previously described for the synthesis of farnesyl-'H pyrophosphate (18 (Table II).…”

Bioconversion of Lignocellulose: Inhibitors and Detoxifi cation

“…The synthesis of both compounds was optimized in the mid80s by Davisson and Poulter during their investigations for improving the diphosphorylation step of the allylic 110 or non-allylic 111 prenyl alcohols over previously published reports [89]. Briefly, the authors found that when pyrophosphoric acid was treated with tetran-butyl ammonium hydroxide and immediately lyophilized, tris(tetra-n-butyl) ammonium hydrogen pyrophospate was obtained on a multigram scale at near quantitative yields for the displacement reaction with the corresponding activated allylic 112 and nonallylic 113 prenyl moieties.…”

Glycosylation in vitro of Semliki‐Forest‐Virus and Influenza‐Virus Glycoproteins and Its Suppression by Nucleotide‐2‐deoxy‐hexose

“…The phosphorylation o f the dolichols was carried out according to Popjak et al [20] based on the method described by Cramer and Bohm [21]. The resulting dolichol pyrophosphate was dephosphorylated to the monophosphate by hydrolysis in 1 M HCl in chloroform/methanol(2/1, v/v) for 1 h at 20 "C 1191.…”