Synthese regiospecifique d'un dinucleoside ponte : (O6- desoxyguanosinyl)-1 (N4-desoxycytidyl)-2 ethane

Maggio, A.‐F.; Lucas, Marc; Barascut, J. L.; Imbach, Jean‐Louis

doi:10.1016/s0040-4039(01)91006-8

Cited by 9 publications

(1 citation statement)

References 18 publications

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“…The 1,2,4-triazole moiety was displaced with ethylene diamine as reported by Sung (17) and Maggio et al (18). Compound 2 was dissolved in 200 ml of CH3CN and added to a solution containing 25 ml of ethylene diamine in 200 ml of CHaCN on ice.…”

Section: Methodsmentioning

confidence: 99%

A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes

Urdea¹,

Warner²,

Running³

et al. 1988

Nucl Acids Res

231

103

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N4-[N-(6-trifluoroacetylamidocaproyl)-2-aminoethyl]-5'-O-dimethoxy trityl -5-methyl-2'-deoxycytidine-3'-N,N-diisopropyl-methylphosphoramidite++ + has been synthesized. This N4-alkylamino deoxycytidine derivative has been incorporated into oligonucleotide probes during chemical DNA synthesis. Subsequent to deprotection and purification, fluorescent (fluorescein, Texas Red and rhodamine), chemiluminescent (isoluminol), and enzyme (horseradish peroxidase, alkaline phosphatase) labels have been specifically incorporated. Detection limits of the labels and labeled probes were assessed. Also, the detection limits and nonspecific binding of the labeled probes in sandwich hybridization assays were determined. The enzyme modified oligonucleotides were found to be significantly better labeling materials than the fluorescent or chemiluminescent derivatives, providing sensitivities comparable to 32P-labeled probes.

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Section: Methodsmentioning

confidence: 99%

A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes

Urdea¹,

Warner²,

Running³

et al. 1988

Nucl Acids Res

231

103

View full text Add to dashboard Cite

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ChemInform Abstract: REGIOSPECIFIC SYNTHESIS OF A BRIDGED DINUCLEOSIDE: 1‐(O6‐DEOXYGUANOSINYL)‐2‐(N4‐DEOXYCYTIDYL)ETHANE

Maggio¹,

Lucas²,

Barascut³

et al. 1984

Chemischer Informationsdienst

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Formation of a tricyclic nucleoside from deoxyguanosine via an O⁶‐N¹‐transposition

Maggio

Lucas

Barascut

et al. 1986

Journal of Heterocyclic Chem

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Synthese regiospecifique d'un dinucleoside ponte : (O6- desoxyguanosinyl)-1 (N4-desoxycytidyl)-2 ethane

Cited by 9 publications

References 18 publications

A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes

A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes

ChemInform Abstract: REGIOSPECIFIC SYNTHESIS OF A BRIDGED DINUCLEOSIDE: 1‐(O6‐DEOXYGUANOSINYL)‐2‐(N4‐DEOXYCYTIDYL)ETHANE

Formation of a tricyclic nucleoside from deoxyguanosine via an O⁶‐N¹‐transposition

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Synthese regiospecifique d'un dinucleoside ponte : (O6- desoxyguanosinyl)-1 (N4-desoxycytidyl)-2 ethane

Cited by 9 publications

References 18 publications

A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes

A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes

ChemInform Abstract: REGIOSPECIFIC SYNTHESIS OF A BRIDGED DINUCLEOSIDE: 1‐(O6‐DEOXYGUANOSINYL)‐2‐(N4‐DEOXYCYTIDYL)ETHANE

Formation of a tricyclic nucleoside from deoxyguanosine via an O6‐N1‐transposition

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Formation of a tricyclic nucleoside from deoxyguanosine via an O⁶‐N¹‐transposition