A new species from montane Colombia is described and illustrated: Gonolobus murphyae (Apocynaceae, Asclepiadoideae, Gonolobinae). The new species is morphologically similar to G. antioquensis (both exhibit caudate corolla lobes), but can be differentiated by a number of characters including vegetative pubescence, calyx pubescence, flower size, and shape of the laminar dorsal anther appendages.