Synergy in antibacterial activities of Ampicillin trihydrate, stabilized with a synthetic aluminum-magnesium silicate and immune-stimulants, on resistant <i>Escherichia coli</i> infection

Abstract

To treat resistant Escherichia coli infection in chicks, Ampicillin was stabilized with a synthetic aluminum-magnesium silicate (AMS) to prolong its bioavailability. Its dose was also reduced to minimize adverse side effects. Vitamins A, C, E and Selenium levels in the chicks' feeds were increased to enhance immune response of the chicks. E. coli coloning forming units, per ml of bile of the chicks, treated with Ampicillin and with Ampicillin in AMS, were: 228800.00 ± 90103.50 and 134500.00 ± 44937.97 at 10 mg/kg, 104400.00 ± 36024.44 and 34800.00 ± 8014.97 at 7.5 mg/kg, 198400.00 ± 129301.80 and 156800.00 ± 109392.70 at 5 mg/kg. Mean bacterial loads of the untreated groups, fed normal feed and those fed the fortified feed were 824400.00 ± 322424.80 and 534800.00 ± 277832.80. At 7.5 mg/kg. Ampicillin in the AMS, effectively (P < 0.05) treated resistant E. coli infection in chicks, fed immunestimulants, with the infection rate reduced by 95.8%.

&lt;i&gt;In Vivo&lt;/i&gt; Antiretroviral Effects of the Medicinal Synthetic Aluminum-Magnesium Silicate

“…This suggests that the AMS-Nanoparticles destroyed infected cells and so released intracellular HIV particles before adsorbing them out, which reduced the titer (P < 0.05) from 14.00 ± 2.00 to 6.50 ± 1.50, when the incubation was repeated [17]. Also, the MSAMS has been observed to enhance efficacy of Sulphadimidine, Chloroquine, Ampicillin and Piparazine stabilized with it [28]- [32]. So, it helps antimicrobials to maximally reduce loads of secondary infections in organs and tissues of treated patients.…”

“…So, most people who claimed invention of cures for HIV/AIDS were not able to scientifically explain mechanisms of actions of the medicines. Development of the passive hemagglutination test for HIV [26] made in vitro test of antiretroviral effects of the MSAMS, possible [6].…”

“…HIV/AIDS patients who requested to try the MSAMS (after it was patented) were made to read the articles titled "Assessment of antiretroviral effects of a synthetic Aluminum-magnesium silicate" [6] and "Antiretroviral effects of a medicinal synthetic Aluminum-magnesium silicate" [17]. Those who volunteered, by writing through Email, to treat themselves with the medicine were selected for the trial, on condition that they would submit results of viral load tests conducted on their plasma, before and after the treatment.…”

“…Adsorbing out HIV means that millions of new viral particles released from infected cells would be prevented from establishing their own foci of infection in more cells [6]. Thus, HIV would no longer be able to overwhelm the body immune system and the acquired immune-deficiency syndrome (AIDS) stage could be prevented.…”

“…So, the new medicine would be cheap and affordable by low income earners who are the people mostly affected by HIV/AIDS [7]. Since in vitro studies have demonstrated its antiretroviral effects [6] and literature has revealed that it is safe for use to treat both animals and human-beings, the medicine was patented by the Nigerian government and was dispensed to HIV positive individuals who volunteered to try it, for cure of HIV/AIDS.…”