Synergy in a detergent combination results in superior decellularized bovine pericardial extracellular matrix scaffolds

Laker, Leana; Dohmen, Pascal M.; Smit, Francis E.

doi:10.1002/jbm.b.34588

Cited by 16 publications

(23 citation statements)

References 23 publications

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“…However, the surfactant-decellularized pericardium was obtained with retained mechanical properties (Figure 4). It is considered that the combination of SDS and SDC could decellularize the pericardium [25]. A cylindrically formed pericardium was obtained, which exhibited a behavior similar to that of the porcine ACL in tensile testing (Figure 5D).…”

Section: Discussionmentioning

confidence: 93%

In Vitro Tissue Reconstruction Using Decellularized Pericardium Cultured with Cells for Ligament Regeneration

et al. 2022

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Recent applications of decellularized tissues have included the ectopic use of their sheets and powders for three-dimensional (3D) tissue reconstruction. Decellularized tissues are fabricated with the desired functions to employ them to a target tissue. The aim of this study was to develop a 3D reconstruction method using a recellularized pericardium to overcome the difficulties in cell infiltration into tight and dense tissues, such as ligament and tendon tissues. Decellularized pericardial tissues were prepared using the high hydrostatic pressurization (HHP) and surfactant methods. The pericardium consisted of bundles of aligned fibers. The bundles were slightly disordered in the surfactant decellularization method compared to the HHP decellularization method. The mechanical properties of the pericardium were maintained after the HHP and surfactant decellularizations. The HHP-decellularized pericardium was rolled up into a cylindrical formation. Its mechanical behavior was similar to that of a porcine anterior cruciate ligament in tensile testing. NIH3T3, C2C12, and mesenchymal stem cells were adhered with elongation and alignment on the HHP- and surfactant-decellularized pericardia, with dependences on the cell type and decellularization method. When the recellularized pericardium was rolled up into a cylinder formation and cultured by hanging circulation for 2 days, the cylinder formation and cellular elongation and alignment were maintained on the decellularized pericardium, resulting in a layer structure of cells in a cross-section. According to these results, the 3D-reconstructed decellularized pericardium with cells has the potential to be an attractive alternative to living tissues, such as ligament and tendon tissues.

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Section: Discussionmentioning

confidence: 93%

In Vitro Tissue Reconstruction Using Decellularized Pericardium Cultured with Cells for Ligament Regeneration

et al. 2022

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“…It should be noted that in a very recent paper, Laker et al ( 167 ) reinforce the concept of synergy achieved with combinations of detergents for decellularization.…”

Section: Treatment Of Xenogeneic Pericardium Before Clinical Implantationmentioning

confidence: 84%

Long-Term Stability and Biocompatibility of Pericardial Bioprosthetic Heart Valves

Williams

Bezuidenhout

Villiers

et al. 2021

Front. Cardiovasc. Med.

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The use of bioprostheses for heart valve therapy has gradually evolved over several decades and both surgical and transcatheter devices are now highly successful. The rapid expansion of the transcatheter concept has clearly placed a significant onus on the need for improved production methods, particularly the pre-treatment of bovine pericardium. Two of the difficulties associated with the biocompatibility of bioprosthetic valves are the possibilities of immune responses and calcification, which have led to either catastrophic failure or slow dystrophic changes. These have been addressed by evolutionary trends in cross-linking and decellularization techniques and, over the last two decades, the improvements have resulted in somewhat greater durability. However, as the need to consider the use of bioprosthetic valves in younger patients has become an important clinical and sociological issue, the requirement for even greater longevity and safety is now paramount. This is especially true with respect to potential therapies for young people who are afflicted by rheumatic heart disease, mostly in low- to middle-income countries, for whom no clinically acceptable and cost-effective treatments currently exist. To extend longevity to this new level, it has been necessary to evaluate the mechanisms of pericardium biocompatibility, with special emphasis on the interplay between cross-linking, decellularization and anti-immunogenicity processes. These mechanisms are reviewed in this paper. On the basis of a better understanding of these mechanisms, a few alternative treatment protocols have been developed in the last few years. The most promising protocol here is based on a carefully designed combination of phases of tissue-protective decellularization with a finely-titrated cross-linking sequence. Such refined protocols offer considerable potential in the progress toward superior longevity of pericardial heart valves and introduce a scientific dimension beyond the largely disappointing ‘anti-calcification’ treatments of past decades.

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“…Decellularized homografts (Group 2, n = 5) were prepared according to our proprietary protocol [ 24 , 25 ] with the addition of Benzonase ® (Thermo Fisher Scientific, Johannesburg, South Africa ). Briefly, homografts were subjected to osmotic shock in two changes of hypertonic 5% NaCl-solution and distilled water, repeated changes in a multi-detergent solution (0.5% Sodium deoxycholate (SDS) (Sigma-Aldrich, Johannesburg, South Africa)), 1% Sodium deoxycholate (SDC) (Sigma-Aldrich, Johannesburg, South Africa), and 1% Triton-X 100 (Sigma-Aldrich, Johannesburg, South Africa)] in PBS, followed by numerous washings in PBS, and a half-strength antibiotic cocktail, which consisted of the same antibiotic mixture that was used for the sterilization of cryopreserved valves, under constant shaking.…”

Section: Methodsmentioning

confidence: 99%

“…The aim of this study was to compare the morphology and mechanical properties of standard cryopreserved pulmonary homografts to decellularized pulmonary homografts and decellularized pulmonary homografts treated with EnCap™ AC technology, evaluating the impact of the processing method on ovine homografts harvested after 48 h cold ischaemia. Our proprietary decellularization protocol with proven synergy was used [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Three Different Processing Techniques on the Strength and Structure of Juvenile Ovine Pulmonary Homografts

et al. 2022

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Homografts are routinely stored by cryopreservation; however, donor cells and remnants contribute to immunogenicity. Although decellularization strategies can address immunogenicity, additional fixation might be required to maintain strength. This study investigated the effect of cryopreservation, decellularization, and decellularization with additional glutaraldhyde fixation on the strength and structure of ovine pulmonary homografts harvested 48 h post-mortem. Cells and cellular remnants were present for the cryopreserved group, while the decellularized groups were acellular. The decellularized group had large interfibrillar spaces in the extracellular matrix with uniform collagen distribution, while the additional fixation led to the collagen network becoming dense and compacted. The collagen of the cryopreserved group was collapsed and appeared disrupted and fractured. There were no significant differences in strength and elasticity between the groups. Compared to cryopreservation, decellularization without fixation can be considered an alternative processing technique to maintain a well-organized collagen matrix and tissue strength of homografts.

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Synergy in a detergent combination results in superior decellularized bovine pericardial extracellular matrix scaffolds

Cited by 16 publications

References 23 publications

In Vitro Tissue Reconstruction Using Decellularized Pericardium Cultured with Cells for Ligament Regeneration

In Vitro Tissue Reconstruction Using Decellularized Pericardium Cultured with Cells for Ligament Regeneration

Long-Term Stability and Biocompatibility of Pericardial Bioprosthetic Heart Valves

Impact of Three Different Processing Techniques on the Strength and Structure of Juvenile Ovine Pulmonary Homografts

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