Synergistic interaction between galectin-3 and carcinoembryonic antigen promotes colorectal cancer metastasis

Wu, Keng‐Liang; Huang, Eng‐Yen; Yeh, Wen‐Ling; Hsiao, Chang‐Chun; Kuo, Chien-Lin

doi:10.18632/oncotarget.18721

Cited by 16 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been proved that a high level of serum galectin-3 correlates with an advanced stage and poor survival in colon cancer patients. 31,32 In our work, we examined four colon cancer cell lines representing different stages of tumor development. HT29 was isolated from a primary tumor, consistent with Dukes' type A classification.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Colon cancer–derived conditioned medium induces differentiation of THP-1 monocytes into a mixed population of M1/M2 cells

et al. 2018

View full text Add to dashboard Cite

Macrophages play an important role in the immune response and in the maintenance of tissue homeostasis. It is well known that many tumors recruit monocytes from circulation and influence their differentiation, mainly into suppressive M2-like subsets. Since there are contradictory data concerning the importance of macrophages for colon cancer progression, we used in our experiments four colon cancer cell lines representing different stages of tumor development (HT29, LS180, SW948, SW620). An acute monocytic leukemia cell line THP-1 was used as a human model of monocytes. Our work revealed that conditioned medium from the tumor cell lines induced activation and differentiation of THP-1 cells. The changes involved increased expression of CD68, a macrophage differentiation marker. Moreover, we also observed increased expression of CD206 and CD163, which are widely considered as markers of tumor-associated macrophages. The tumor-derived conditioned medium decreased the proliferation of THP-1 cells and blocked their cell cycle at the G1 stage. The tumor-conditioned medium also upregulated the production of several cytokines and chemokines characteristic of both M1 and M2 subsets and induced the expression of important pro-angiogenic factors, vascular endothelial growth factor, and matrix metalloproteinase-9 in THP-1 cells. Moreover, the tumor-conditioned medium induced the expression of galectin-3, which is implicated in malignant transformation, and indoleamine 2,3-dioxygenase, that is, a key enzyme of the kynurenine pathway. Our data suggest that tumor cells can actively influence the phenotype of monocytes and switch their differentiation into a population of non-adherent mixed M1 and M2 cells. These preliminary studies suggest that colon cancer cells produce soluble factors that influence monocyte differentiation, most probably into suppressive subsets. These data provide a better understanding of the influence of colon cancer on polarization of monocytes.

show abstract

Section: Discussionmentioning

confidence: 99%

“…It has been proved that a high level of serum galectin-3 correlates with an advanced stage and poor survival in colon cancer patients. 31,32…”

Section: Discussionmentioning

confidence: 99%

Colon cancer–derived conditioned medium induces differentiation of THP-1 monocytes into a mixed population of M1/M2 cells

et al. 2018

View full text Add to dashboard Cite

show abstract

“…It has been reported that cancer-associated mutations in HRas, KRas, and NRas oncoproteins increase their activity through augmented nanoclustering of Ras on membranes [22]. Similarly, Ras clustering modulation by the overexpression of various galectins (e.g., Galectin-1 and Galectin-3), which is observed in breast, pancreatic, colorectal, and other cancers, can increase Ras nanoclustering and its oncogenic output [24, 246, 248–251]. Likewise, nanoclustering of other plasma membrane components, i.e., carbohydrates and lipids, plays an important role in cancer.…”

Section: Dysregulation Of Plasma Membrane Biochemical and Biophysicalmentioning

confidence: 99%

Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents

Erazo-Oliveras

Fuentes

Wright

et al. 2018

Cancer Metastasis Rev

View full text Add to dashboard Cite

The cell plasma membrane serves as a nexus integrating extra- and intracellular components, which together enable many of the fundamental cellular signaling processes that sustain life. In order to perform this key function, plasma membrane components assemble into well-defined domains exhibiting distinct biochemical and biophysical properties that modulate various signaling events. Dysregulation of these highly dynamic membrane domains can promote oncogenic signaling. Recently, it has been demonstrated that select membrane-targeted dietary bioactives (MTDBs) have the ability to remodel plasma membrane domains and subsequently reduce cancer risk. In this review, we focus on the importance of plasma membrane domain structural and signaling functionalities as well as how loss of membrane homeostasis can drive aberrant signaling. Additionally, we discuss the intricacies associated with the investigation of these membrane domain features and their associations with cancer biology. Lastly, we describe the current literature focusing on MTDBs, including mechanisms of chemoprevention and therapeutics in order to establish a functional link between these membrane-altering biomolecules, tuning of plasma membrane hierarchal organization, and their implications in cancer prevention.

show abstract

“…In CRC, AFP and CEA have been used as reliable tumor markers for monitoring tumor progression. Recent studies demonstrated that Gal-3 interacts with CEA, promoting colorectal cancer cell migration, adhesion, and subsequent metastasis [ 22 , 57 ]. Analysis on UC patients revealed that fecal Gal-3 does not correlate with the endoscopic and clinical Mayo score, as well as with histopathological parameters of the affected tissue, and is not suitable for evaluation of severity of UC ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…It is shown that patients with detectable expression of Gal-3 in tumor have more lymph node and distant metastases, frequent venous invasion, and deeper wall invasion in comparison to those with Gal-3-negative cases [ 21 ]. Moreover, a recent study revealed that serum galectin-3 and carcinoembryonic antigen (CEA) promote CRC migration and metastasis [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Fecal Galectin-3: A New Promising Biomarker for Severity and Progression of Colorectal Carcinoma

Jovanovic

Gajovic

Zdravković

et al. 2018

Mediators of Inflammation

View full text Add to dashboard Cite

Background and Objectives The aim of the study was to determine systemic and fecal values of galectin-3 and pro- and anti-inflammatory cytokines in patients with CRC and the relationship with clinicopathological aspects. Methods Concentrations of galectin-3, TNF-α, TGF-β, IL-10, and IL-1β were analyzed in samples of blood and stool of 60 patients with CRC. Results Systemic concentration of TNF-α was significantly lower in patients with severe diseases (advanced TNM stage, nuclear grade, and poor histological differentiation) as in patients with more progressive CRC (lymph and blood vessel invasion, presence of metastasis). Fecal values of anti-inflammatory cytokines TGF-β and IL-10 were increased in patients with severe stadium of CRC. Fecal concentration of Gal-3 was enhanced in CRC patients with higher nuclear grade, poor tumor tissue differentiation, advanced TNM stage, and metastatic disease. Gal-3/TNF-α ratio in sera and feces had a higher trend in patients with severe and advanced diseases. Positive correlation between fecal Gal-3 and disease severity, tumor progression, and biomarkers AFP and CEA, respectively, was also observed. Conclusions Predomination of Gal-3 in patients with advanced diseases may implicate on its role in limiting ongoing proinflammatory processes. The fecal values of Gal-3 can be used as a valuable marker for CRC severity and progression.

show abstract

Synergistic interaction between galectin-3 and carcinoembryonic antigen promotes colorectal cancer metastasis

Cited by 16 publications

References 25 publications

Colon cancer–derived conditioned medium induces differentiation of THP-1 monocytes into a mixed population of M1/M2 cells

Colon cancer–derived conditioned medium induces differentiation of THP-1 monocytes into a mixed population of M1/M2 cells

Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents

Fecal Galectin-3: A New Promising Biomarker for Severity and Progression of Colorectal Carcinoma

Contact Info

Product

Resources

About