Synergistic enhancement of tolerance mechanisms in response to photoactivation of cationic tetra (N-methylpyridyl) porphyrins in tomato plantlets

Guillaumot, Damien; Issawi, Mohammad; Silva, Anne Da; Leroy‐Lhez, Stéphanie; Sol, Vincent; Riou, Catherine

doi:10.1016/j.jphotobiol.2016.01.015

Cited by 20 publications

(28 citation statements)

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“…Furthermore, cationic porphyrins have been shown to be able to reach root meristematic cell nucleus and induced DNA photodamage (Villanueva et al ). Recently and in the present work, we showed that cationic porphyrins at 3.5 μ M were able to inhibit Arabidopsis early vegetative development whereas tomato plantlets treated with 80 μ M CP/CP‐Zn were also affected but could be rescued (Guillaumot et al ). This result was in agreement to the necessity of a case‐by‐case study because photosensitizer effects depend on the ability to be taken up by plants and to enter into the cells.…”

Section: Discussionmentioning

confidence: 51%

“…For spectral acquisition, samples were excited at 458 nm and fluorescence was followed between 476 and 732 nm. CP‐Zn emission peak was detected around 640 nm (Guillaumot et al ). For channel mode acquisition and calcofluor, we used an excitation wavelength at 405 nm and emission wavelength between 420 and 450 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Total thiol assay was performed according to (Guillaumot et al ). Frozen samples were ground in liquid nitrogen and then extracted into 1 ml of 0.2 N HCl.…”

Section: Methodsmentioning

confidence: 99%

“…It could be envisaged as a farming practice in order to eradicate plant pathogens as bacteria and fungi that cause serious threats by drastically reducing crops yield. Studies focusing on the side effects of APDT directly on plants are rare (De Menezes et al , , Fracarolli et al , De Menezes et al , Guillaumot et al , Gonzales et al 2017). However, such studies are required to evaluate the efficiency of APDT.…”

Section: Introductionmentioning

confidence: 99%

“…On Allium cepa root meristematic cells it was reported that zinc metalated/free base cationic porphyrins were able to be up‐taken by cells and induce DNA photodamage (Villanueva et al ). More recently, study on tomato plantlets showed that they were able to withstand the damaging effects of photoactivated cationic porphyrins (Guillaumot et al ). As pointed out by these studies, we propose that exogenous photoactivated porphyrins could seriously alter plant development leading to preferentially death of fast growing species such as A. thaliana .…”

Section: Introductionmentioning

confidence: 99%

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Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Issawi

Guillaumot

Sol

et al. 2017

Physiologia Plantarum

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Antimicrobial photodynamic treatment (APDT) based on the use of a photosensitizer to produce reactive oxygen species (ROS) that induce cell death could be envisaged to fight against plant pathogens. For setting this strategy, we want to study how plants themselves respond to photodynamic treatment. In previous work we showed that tomato plantlets were able to resist photoactivated tetra (N-methylpyridyl) porphyrin (CP) or the zinc metalated form (CP-Zn). To enlarge our plant expertise related to exogenous porphyrins treatment and to further defend this approach, we studied how a weed like Arabidopsis thaliana responded to exogenous supply of anionic and cationic porphyrins. Both types of photosensitizers had no negative effect on seed germination and did not hamper the development etiolated Arabidopsis plantlet under dark conditions. Thus, post-emergence effects of porphyrin photoactivation on the development of 14 day-old in vitro Arabidopsis plantlet under light were observed. CP-Zn was the most efficient photosensitizer to kill Arabidopsis plantlets while anionic tetra (4-sulfonatophenyl) porphyrin only delayed their growth and development. Indeed only 7% of plantlets could be rescued after CP-Zn treatment. Furthermore, non-enzymatic and enzymatic defense components involved in detoxification of ROS generated by CP-Zn under illumination were downregulated or stable with the exception of sevenfold increase in proline content. As previously demonstrated in the literature for microbial agents and in the present work for Arabidopsis, CP-Zn was efficient enough to eradicate unwanted vegetation and plant pathogens without at the same time killing plants of agronomic interest such as tomato plantlets.

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Section: Discussionmentioning

confidence: 51%

Section: Methodsmentioning

confidence: 99%

“…Total thiol assay was performed according to (Guillaumot et al ). Frozen samples were ground in liquid nitrogen and then extracted into 1 ml of 0.2 N HCl.…”

Section: Methodsmentioning

confidence: 99%