Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities

Selig, Michael S.; Knoshaug, Eric P.; Adney, William S.; Himmel, Michael E.; Decker, Stephen R.

doi:10.1016/j.biortech.2007.09.064

Cited by 220 publications

(150 citation statements)

References 36 publications

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“…-glucosidase is one of the most widely reported synergistic effects of the cellobiohydrolase from T. reesei (Zhang et al, 2006). Selig et al reported that the addition of xylanolytic enzymes generally enhanced the performance of the cellobiohydrolase from T. reesei on pretreated biomass, and the xylan fraction more directly occluded the cellulose in pretreated lignocelluloses and showed higher cellulase activity (Selig et al, 2008(Selig et al, , 2009). Furthermore, the addition of the xylanolytic enzyme and -glucosidase are very important for enhancing the performance of the cellobiohydrolase from T. reesei on the pretreated biomass.…”

Section: Enzymatic Hydrolysis Of Ahp-treatedmentioning

confidence: 99%

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Hideno

Kawashima

Anzoua

et al. 2013

Bioresource Technology

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The diploid Miscanthus sinensis "Shiozuka" which was selected as a high-biomass producing line, and the triploid M. × giganteus (M×G) were treated by ball milling (physical treatment) and alkaline hydrogen peroxide treatment (AHP; chemical treatment), and their structural sugar compositions and enzymatic digestibility were compared. The structural sugar content of Shiozuka was moderate and lower than that of M×G. The Klason lignin content of Shiozuka was also lower than that of M×G.However, Shiozuka was sensitive to ball milling and AHP treatment; ball milled and AHP-treated Shiozuka had higher enzymatic digestibility than ball milled and AHPtreated M×G. Shiozuka would be promising feedstock to obtain fermentable sugars with low energy consumption. Finally, enzymes for the hydrolysis of chemically treated Miscanthus were isolated from Trichoderma reesei ATCC 66589 and Penicillium pinophilum. The sugar yield could be increased by enzymatic hydrolysis of AHP-treated samples with NaOH and H 2 O 2 and the isolated enzymes. Highlights> Miscanthus sinensis "Shiozuka" contained moderate sugar but low lignin contents.> Shiozuka was more sensitive to physical and chemical treatments than M×G. >Enzymatic digestibilities of the pretreated Shiozuka were higher than those of M×G.>Shiozuka is a promising bioenergy grass cultivar.3

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Section: Enzymatic Hydrolysis Of Ahp-treatedmentioning

confidence: 99%

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Hideno

Kawashima

Anzoua

et al. 2013

Bioresource Technology

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“…Acetyl xylan (O-acetyl-4-O-methyl-Dglucurono- D-xylan) is the naturally occurring form of hemicellulose in hardwood, alternate xylopyranosyl units of the polymeric xylan contains one acetyl group (Biely et al 1986; Biely 2012). Selig et al (2008) and has showed that combinatorial action of endoxylanases and AcXE has significantly improved the hydrolysis of xylan polymer (Selig et al 2008). Selig et al (2009) have reported that when AcXEs were used in combination with endoxylanases on different corn stover substrates, a linear relationship in removal of acetyl groups and depolymerisation of xylan was observed (Selig et al 2009).…”

Section: Microbial Enzymes Depolymerisation Of Hemicellulosementioning

confidence: 99%

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases

Kameshwar

Qin

2018

Mycology

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Acetyl and methyl esterifications are two major naturally found substitutions in the plant cell-wall polysaccharides. The non-cellulosic plant cell-wall polysaccharides such as pectin and hemicellulose are differentially esterified by the O-acetyl and methyl groups to cease the action of various hydrolytic enzymes secreted by different fungi and bacterial species. Thus, microorganisms have emerged with a special class of enzymes known as carbohydrate esterases (CE). The CE catalyse O-de, N-deacetylation of acetylated saccharide residues (esters or amides, where sugars play the role of alcohol/amine/acid). Carbohydrate active enzyme (CAZy) database has classified CE into 16 classes, of which hemicellulose deacetylating CE were grouped into eight classes (CE-1 to CE-7 and CE-16). Various plant biomass degrading fungi and bacteria secretes acetyl xylan esterases (AcXE); however, these enzymes exhibit varied substrate specificities. AcXE and xylanases-coupled pretreatment methods exhibit significant applications, such as enhancing animal feedstock, baking industry, production of food additives, paper and pulp, xylitol production and biorefinery-based industries, respectively. Thus, understanding the structural and functional properties of acetyl xylan esterase will significantly aid in developing the efficient AcXE with wide range of industrial applications.

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“…The most prominent potential application of AcXEs is specifically in the saccharification of acetylated hemicellulose extracted from plant biomass under non-alkaline conditions [148][149][150]. The potential role of AcXEs in enhanced lignocellulosic biomass degradation processes has been extensively reviewed [3,5,6,9,19,[151][152][153][154][155].…”

Section: Applications Of Acxesmentioning

confidence: 99%

Phylogeny, classification and metagenomic bioprospecting of microbial acetyl xylan esterases

Adesioye

Makhalanyane

Biely

et al. 2016

Enzyme and Microbial Technology

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Highlights• AcXEs occur in several CAZy families, and show promiscuous substrate specificities.• A strong AcXE sequence to specificity link would be a valuable functional predictor.• There is a clear need for novel AcXE enzymes with detailed substrate specificity data.• Functional metagenomics would be the most effective means for identifying new AcXEs.• A lack of suitable substrates limits high throughput metagenomic biomining of AcXEs.

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Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities

Cited by 220 publications

References 36 publications

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases

Phylogeny, classification and metagenomic bioprospecting of microbial acetyl xylan esterases

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