Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Abstract

The diploid Miscanthus sinensis "Shiozuka" which was selected as a high-biomass producing line, and the triploid M. × giganteus (M×G) were treated by ball milling (physical treatment) and alkaline hydrogen peroxide treatment (AHP; chemical treatment), and their structural sugar compositions and enzymatic digestibility were compared. The structural sugar content of Shiozuka was moderate and lower than that of M×G. The Klason lignin content of Shiozuka was also lower than that of M×G.However, Shiozuka was sensitive to ball milling and AHP treatment; ball milled and AHP-treated Shiozuka had higher enzymatic digestibility than ball milled and AHPtreated M×G. Shiozuka would be promising feedstock to obtain fermentable sugars with low energy consumption. Finally, enzymes for the hydrolysis of chemically treated Miscanthus were isolated from Trichoderma reesei ATCC 66589 and Penicillium pinophilum. The sugar yield could be increased by enzymatic hydrolysis of AHP-treated samples with NaOH and H 2 O 2 and the isolated enzymes. Highlights> Miscanthus sinensis "Shiozuka" contained moderate sugar but low lignin contents.> Shiozuka was more sensitive to physical and chemical treatments than M×G. >Enzymatic digestibilities of the pretreated Shiozuka were higher than those of M×G.>Shiozuka is a promising bioenergy grass cultivar.3

Thermal degradation behavior of ball-milled Miscanthus plants and its relationship to enzymatic hydrolysis

“…M. sinensis and M. giganteus were used similarly in the author's previous report (Hideno et al 2013). Each sample was hand-cut to approximately 1 cm to 2 cm in length, and dry milled using a blender (ABSOLUTE3, Osaka Chemical Co., Ltd., Osaka, Japan).…”

“…Moreover, based on DTG data, it was indicated that the effects of ball milling on non-cellulosic substances, such as hemicellulose and lignin, were more significant than those on crystalline cellulose. We also previously described the enzymatic hydrolysis of two ball-milled Miscanthus species (M. giganteus and M. sinensis) (Hideno et al 2013), which are promising bioenergy crops with high biomass production potential because they are C4 plants (Clifton-Brown et al 2004;Anzoua et al 2011).…”

Thermogravimetric analysis-based characterization of suitable biomass for alkaline peroxide treatment to obtain cellulose and fermentable sugars

“…Pretreatment for enzymatic hydrolysis of lignocellulose includes a similar pulping process (Mosier et al 2005). Several studies using pretreatments to obtain cellulose and fermentable sugars from agricultural residues including rice straw and herbaceous biomass such as Miscanthus plants have been conducted (Mosier et al 2005;Hendriks and Zeeman 2008;Hideno et al 2009Hideno et al , 2013b. Other reported pretreatments include milling, grinding, and treatments with sulfuric acid, alkalis, and biological agents such as white-rot fungus (Hendriks and Zeeman 2008;Hideno et al 2009Hideno et al , 2013b.…”

“…Several studies using pretreatments to obtain cellulose and fermentable sugars from agricultural residues including rice straw and herbaceous biomass such as Miscanthus plants have been conducted (Mosier et al 2005;Hendriks and Zeeman 2008;Hideno et al 2009Hideno et al , 2013b. Other reported pretreatments include milling, grinding, and treatments with sulfuric acid, alkalis, and biological agents such as white-rot fungus (Hendriks and Zeeman 2008;Hideno et al 2009Hideno et al , 2013b. Alkaline-peroxide (AP) treatment is simple and highly effective at removing lignin and hemicellulose, resulting in a high sugar yield in the enzymatic hydrolysis.…”

Production of Sugar Feedstocks for Fermentation Processes from Selected Fast Growing Grasses

“…Studies on compositional changes of its biomass over the harvesting season showed that it is possible to optimize the harvesting process to ensure the complete biomass utilization for a variety of products [19,20]. Miscanthus is a popular fast growing nonfood feedstock, widely applied not only as an energy crop, but also as livestock feed and organic fertilizer [21,22].…”