Synergistic effects of ultraviolet-B and methyl jasmonate on tanshinone biosynthesis in Salvia miltiorrhiza hairy roots

Wang, Cong Hui; Li, Zheng; Tian, Hao; Wang, Jian Wen

doi:10.1016/j.jphotobiol.2016.01.012

Cited by 52 publications

(43 citation statements)

References 29 publications

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“…The combined treatment of UV-B irradiation and MeJA exhibited synergistic effects on the expression levels of 3-hydroxy-3-methylglutaryl-CoA reductase ( SmHMGR ) involved in MVA pathway and geranylgeranyl diphosphate synthase ( SmGGPPS ) genes in the tanshinone biosynthetic pathway (Wang et al, 2016). All MVA pathway related genes from S. miltiorrhiza exhibited a significant increase in expression levels at 12 hpi, but this was followed by a significant drop at 24 hpi, and a return to expression levels only slightly higher than the control (0 hpi) at 36 hpi, representing a rapid but transient response to elicitation (YE+ Ag + ; Gao et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Functional Analysis of Squalene Synthase 2(SQS2) in Salvia miltiorrhiza Bunge

et al. 2016

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Salvia miltiorrhiza Bunge, which is also known as a traditional Chinese herbal medicine, is widely studied for its ability to accumulate the diterpene quinone Tanshinones. In addition to producing a variety of diterpene quinone, S. miltiorrhiza Bunge also accumulates sterol, brassinosteroid and triterpenoids. During their biosynthesis, squalene synthase (SQS, EC 2.5.1.21) converts two molecules of the hydrophilic substrate farnesyl diphosphate (FPP) into a hydrophobic product, squalene. In the present study, cloning and characterization of S. miltiorrhiza Bunge squalene synthase 2 (SmSQS2, Genbank Accession Number: KM408605) cDNA was investigated subsequently followed by its recombinant expression and preliminary enzyme activity. The full-length cDNA of SmSQS2 was 1 597 bp in length, with an open reading frame of 1 245 bp encoding 414 amino acids. The deduced amino acid sequence of SmSQS2 shared high similarity with those of SQSs from other plants. To obtain soluble recombinant enzymes, the truncated SmSQS2 in which 28 amino acids were deleted from the carboxy terminus was expressed as GST-Tag fusion protein in Escherichia coli BL21 (DE3) and confirmed by SDS-PAGE and Western Blot analysis, and the resultant bacterial crude extract was incubated with FPP and NADPH. Gas chromatograph-mass spectrometer analysis showed that squalene was detected in the in vitro reaction mixture. The gene expression level was analyzed through Quantitative real-time PCR, and was found to be higher in roots as compared to the leaves, and was up-regulated upon YE+ Ag+ treatment. These results could serve as an important to understand the function of the SQS family. In addition, the identification of SmSQS2 is important for further studies of terpenoid and sterol biosynthesis in S. miltiorrhiza Bunge.

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Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Functional Analysis of Squalene Synthase 2(SQS2) in Salvia miltiorrhiza Bunge

et al. 2016

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“…Furthermore, a combined approach using both types of elicitors can be employed. For instance Wang et al (2016) used ultraviolet-B (UV-B) radiation and methyl jasmonate applied alone or in combination in Salvia miltiorrhiza hairy root cultures and found that the combined treatment exhibited synergistic effects on the expression levels of genes in the tanshinone biosynthetic pathway.…”

Section: Alternatives To In Vitro Plant Culturementioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

417

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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“…UV radiation (UV-A, UV-B, and UV-C) has been used as an abiotic elicitor in HRC of several species to enhance different groups of SMs [47][48][49][50][51][52]. Like other elicitors, dose, duration, plant species, etc.…”

Section: Abiotic Elicitors 221 Effect Of Uv Radiation On Sm Productionmentioning

confidence: 99%

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Halder

Sarkar

Jha

2019

Engineering in Life Sciences

228

116

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Elicitation is a possible aid to overcome various difficulties associated with the large‐scale production of most commercially important bioactive secondary metabolites from wild and cultivated plants, undifferentiated or differentiated cultures. Secondary metabolite accumulation in vitro or their efflux in culture medium has been elicited in the undifferentiated or differentiated tissue cultures of several plant species by the application of a low concentration of biotic and abiotic elicitors in the last three decades. Hairy root cultures are preferred for the application of elicitation due to their genetic and biosynthetic stability, high growth rate in growth regulator‐free media, and production consistence in response to elicitor treatment. Elicitors act as signal, recognized by elicitor‐specific receptors on the plant cell membrane and stimulate defense responses during elicitation resulting in increased synthesis and accumulation of secondary metabolites. Optimization of various parameters, such as elicitor type, concentration, duration of exposure, and treatment schedule is essential for the effectiveness of the elicitation strategies. Combined application of different elicitors, integration of precursor feeding, or replenishment of medium or in situ product recovery from the roots/liquid medium with the elicitor treatment have showed improved accumulation of secondary metabolites due to their synergistic effect. This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.

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Synergistic effects of ultraviolet-B and methyl jasmonate on tanshinone biosynthesis in Salvia miltiorrhiza hairy roots

Cited by 52 publications

References 29 publications

Molecular Cloning and Functional Analysis of Squalene Synthase 2(SQS2) in Salvia miltiorrhiza Bunge

Molecular Cloning and Functional Analysis of Squalene Synthase 2(SQS2) in Salvia miltiorrhiza Bunge

In vitro plant tissue culture: means for production of biological active compounds

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

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