Synergistic effects of nanoparticle heating and amoxicillin on H. pylori inhibition

Wu, Tao; Wang, Lichen; Gong, Meiliang; Lin, Yunjuan; Xu, Yang; Ye, Ling; Yu, Xingwen; Liu, Jing; Liu, Jianwei; He, Song; Zeng, Hao; Wang, Gangshi

doi:10.1016/j.jmmm.2019.04.076

Cited by 21 publications

(13 citation statements)

References 53 publications

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“…Thermal shock has been demonstrated as a means of deactivating established biofilms but may also damage adjacent tissue [ 33 , 34 ]. Recent studies have suggested a synergism between antibiotics and thermal shock, with the combined treatment decreasing biofilm population density more than either treatment alone, or even by the product of their individual effects [ 35 , 36 , 37 , 38 ]. The nature of this interaction is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms

Aljaafari

Chicchelly

et al. 2021

Antibiotics

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Bacterial biofilm infections are a major liability of medical implants, due to their resistance to both antibiotics and host immune response. Thermal shock can kill established biofilms, and some evidence suggests antibiotics may enhance this efficacy, despite having an insufficient effect themselves. The nature of this interaction is unclear, however, complicating efforts to integrate thermal shock into implant infection treatment. This study aimed to determine whether these treatments were truly synergistic or simply orthogonal (i.e., independent). Pseudomonas aeruginosa biofilms of different architectures and stationary-phase population density were subjected to various thermal shocks, antibiotic exposures, or combinations thereof, and examined either immediately after treatment or after subsequent reincubation. Population decreases from the combination treatment matched the product of the decreases of individual treatments, indicating their orthogonality. However, reincubation showed binary behavior, where biofilms with an immediate population decrease beyond a critical factor (~104) died off completely during reincubation, while biofilms with a smaller immediate decrease regrew. This critical factor was independent of the initial population density and the combination of treatments that achieved the immediate decrease. While antibiotics do not appear to enhance thermal shock directly, their contribution to achieving a critical population decrease for biofilm elimination can make the treatments appear strongly synergistic, strongly decreasing the intensity of thermal shock needed.

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Section: Introductionmentioning

confidence: 99%

Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms

Aljaafari

Chicchelly

et al. 2021

Antibiotics

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“…Recently, Pijls et al [32] investigated the synergistic effects of non-contact induction heating and antibiotic cocktails and reported that a combination of heat plus antibiotics had synergistic effects on Staphylococcus aureus biofilms at 60 C. Both studies achieved promising results; however; they performed their research at high temperatures, which may cause thermal damage to human tissue. Wu et al [33] designed a magnetic nanoparticle platform to investigate the synergistic effects of nanoparticle heating and the antimicrobial drug amoxicillin on H. pylori and reported that this combination increased the susceptibility of H. pylori to amoxicillin due to the destruction of the cell membrane and microbial biofilm at 41 C. In contrast to our study, they used amoxicillin-loaded nanoparticles in their research. In 2020, Alumutairi et al [34] reported that magnetic nanoparticle hyperthermia enhanced the susceptibility of S. aureus biofilm to ciprofloxacin and vancomycin antibiotics.…”

Section: Colony Counts At 1st Hourmentioning

confidence: 69%

In vitro activity of hyperthermia on swarming motility and antimicrobial susceptibility profiles of Proteus mirabilis isolates

Gazel

Demirbakan

Erinmez

2021

International Journal of Hyperthermia

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Aim: Swarming motility is a virulence factor for Proteus mirabilis and is a coordinated multicellular movement of bacteria. In this study, we investigated the inhibitory effect of hyperthermia on bacterial swarming motility and antimicrobial resistance. Methods: Thirty-one P. mirabilis isolates were included in the study. Seven inoculated agar plates were incubated inside incubators with increasing temperature levels: at 36 C (control) and 40-45 C. On the next day, inhibition of swarming was evaluated and minimum paralyzing temperature (MPT) values were determined. An antimicrobial susceptibility test (antibiogram) is performed by exposing bacteria to increasing concentrations of antibiotics, in vitro. Thus, we used the Kirby-Bauer disk diffusion test as a screening method to analyze the antibiogram profiles of the isolates at 36 C and 42 C. Finally, a time-kill assay was performed to analyze the killing effect of hyperthermia (42 C) on planktonic bacteria, in combination with the antibiotic meropenem at the first and third hours. A Wilcoxon signed-rank test was used to compare the killing effects of meropenem, hyperthermia and their combinations. Results: The median MPT value was determined as 44 C. In the disk diffusion assay, susceptibility development was observed in 94% of isolates for at least one antibiotic. In the time-kill assay, we observed a significant killing effect of hyperthermia in combination with meropenem. Under the microscope, we observed the formation of spherical cells by the effect of heat. Conclusion:We conclude that these findings might be useful when employing the hyperthermia method to treat infectious diseases caused by P. mirabilis in the future.

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“…Alteration of antimicrobial susceptibility was reported after hyperthermia on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and our previous study found that NPs magnetic local heating could not only disrupt H. pylori cell directly, but could also enhance its susceptibility to amoxicillin, of which could be adopted for clinic applications readily [44][45][46][47][48]. One crucial mechanism of drug resistance is the formation of bio lms.…”

Section: Discussionmentioning

confidence: 99%

Nanocluster Mediated Photothermia Improves Eradication Efficiency and Antibiotic Sensitivity of Helicobacter Pylori

Meng

Tao

et al. 2021

Preprint

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Helicobacter pylori (H. pylori) eradication plays a crucial role in gastric cancer prevention, but the antimicrobial resistance of H. pylori is obstructing this elimination process. In this study, we developed nanoclusters (NCs) from Zn0.3Fe2.7O4 nanoparticles using a poly(ethylene glycol)-b-poly(ε-caprolactone)-based nanocarrier as an innovative antibiotic-independent H. pylori management. The nanocluster showed minimal toxicity and maximal biocompatibility. With a low concentration (50 µg/mL) of NCs under a short time period (～2 min) of near-infrared (808nm) irradiation, we kept the culture medium temperature to 41 °C for 20 minutes with continuous irradiation. The heated NCs exhibited efficient photothermal effects and resulted in an excellent inhibition of H. pylori growth, adhesion ability and cell vacuolization ability in in vitro investigation. Transmission electron microscopy showed a dramatic morphologic change after NCs photothermia on H. pylori, including cell wall and membrane rupture, as well as ribosome damage. Besides, levofloxacin and clarithromycin resistance were improved after photothermal treatment in H. pylori NCTC 11637 and/or clinical strains, however metronidazole resistance was unchanged. We also discovered a significant decrease in the biofilm formation of H. pylori under the NCs-based photothermal application, while efflux pump function was unchanged. In summary, using this novel NCs-based photothermal approach, we were able to demonstrate in vitro a significant inhibition of both H. pylori growth and molecular toxicity, and its improvement in antibiotic resistance alone with the eradication of H. pylori biofilms previously believed to be resistant to conventional antibiotics.

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Synergistic effects of nanoparticle heating and amoxicillin on H. pylori inhibition

Cited by 21 publications

References 53 publications

Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms

Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms

In vitro activity of hyperthermia on swarming motility and antimicrobial susceptibility profiles of Proteus mirabilis isolates

Nanocluster Mediated Photothermia Improves Eradication Efficiency and Antibiotic Sensitivity of Helicobacter Pylori

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