Synergistic effects of myo‐inositol and melatonin on cryopreservation of goat spermatozoa

Abstract

Overproduction of reactive oxygen species (ROS) during sperm cryopreservation has a detrimental effect on sperm parameters. Therefore, the use of antioxidants in the sperm freezing extender can reduce ROS destructive effects. In this study, we investigated whether co-supplementation of melatonin and myo-inositol into the semen extender can improve the post-cryopreservation quality of goat spermatozoa. After the freeze-thawing process, sperm motility, viability, plasma membrane and acrosome intact morphology were improved in the combined myo-inositol and melatonin group compared to both individual and the control groups (p < .05). In addition, the mean of sperm ROS, DNA damage and lipid peroxidation were reduced in co-supplementation of myo-inositol and melatonin compared to their individual counterparts (p < .05).Therefore, the synergistic effects of myo-inositol and melatonin on the cryopreserved spermatozoa are highly likely mediated through the reduction in important factors involved in the sperm lipid peroxidation. Finally, we used the cryopreserved spermatozoa for in vitro production of embryos. Results showed that combined group of myo-inositol and melatonin improved the cleavage rate compared to both individual and control groups, although blastocyst rate was improved using both individual and combined groups. In conclusion, co-supplementation of melatonin and myo-inositol is a promising approach for the improvement of goat sperm cryopreservation.

Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies

“…Moreover, 5 mM oxidized glutathione increased the motion and velocity characteristics of ram sperm after freeze–thaw [ 92 ], and similar results were found at lower concentrations in turkey sperm [ 93 ]. Similar results have been found using the multifunctional antioxidant melatonin, where positive effects for sperm cryopreservation were found in pig [ 94 ], ram [ 95 ], goat [ 96 ], canine [ 97 ], fish [ 98 ], bovine [ 99 ], and human [ 100 ].…”

Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos

“…Melatonin, a type of indoleamine and free radical scavenger, has been reported to exhibit various antioxidative effects through G-protein-coupled receptors (GPCR). Recently reported studies have focused on the protective effect of melatonin in maintaining fertility in several species of animals and humans [ 25 , 27 , 28 , 29 , 30 , 31 , 34 , 39 , 137 , 138 , 139 ]. Even in humans, melatonin has been reported to be very useful as a sperm cryoprotectant and it has a significant effect on the toxicity and damage recovery of frozen sperm.…”

“…In the ram, melatonin inhibits the mitochondrial permeability transition pore opening by MT1-related PI3K/AKT/GSK3b signal activation [ 34 ]. Additionally, melatonin and Myo-inositol mixture have a critical effect on sperm activation during cryopreservation in goat sperm [ 39 ]. In pigs, a melatonin and butylhydroxytoluene mixture successfully reduced reactive nitrogen species and oxidative stress during a sperm vitrification procedure [ 36 ].…”

“…Melatonin (N-acetyl-5-methoxytryptamine) is a hormone secreted from vertebrate pineal gland and regulates homeostasis of reproductive tissues such as ovary and placenta [ 22 ] through receptor-mediated signaling pathways [ 23 ]. Recent studies have reported that melatonin plays a critical role in the cryopreservation of sperm [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ], oocytes [ 40 , 41 , 42 , 43 , 44 , 45 , 46 ], and embryos [ 47 , 48 , 49 , 50 , 51 ] in various species. In addition, melatonin is an antioxidant that acts as a free radical scavenger and affects G-protein-coupled receptor-related signaling transduction [ 23 , 52 ].…”

Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen

“…9 However, the excessive production of ROS due to the freeze-thawing process cannot be overcome by endogenous antioxidants owing to the limitations of the spermatozoa cytoplasm and decreased antioxidant levels due to the addition of extenders. 10 Several studies to improve the quality of post-thawing goats semen have been carried out, including adding extenders with egg yolk omega-3, 11 fish semen plasma, 12,13 curcumin, 14 butylated hydroxytoluene, 15 combination of myo-inositol and melatonin, 16 and L-carnitine. 17 Epigallocatechin-3-gallate (EGCG) is a powerful antioxidant extracted from green tea.…”