2022
DOI: 10.3390/ani12233271
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Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies

Abstract: Cryopreservation is a way to preserve germplasm with applications in agriculture, biotechnology, and conservation of endangered animals. Cryopreservation has been available for over a century, yet, using current methods, only around 50% of spermatozoa retain their viability after cryopreservation. This loss is associated with damage to different sperm components including the plasma membrane, nucleus, mitochondria, proteins, mRNAs, and microRNAs. To mitigate this damage, conventional strategies use chemical ad… Show more

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Cited by 19 publications
(23 citation statements)
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“…In this study, we aimed to develop the best-performing extender while exploring the interactions between various ingredients. Extender formulations play a significant role in the standard cryopreservation protocol, and each ingredient has a specific function such as cryoprotectant, antioxidant, energy metabolism function, and membrane function during freeze-thaw [ 3 ]. We observed multiple interactions between each individual component, and this is not possible to discover with conventional experimentation such as comparing two components at a time [ 9 ].…”
Section: Resultsmentioning
confidence: 99%
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“…In this study, we aimed to develop the best-performing extender while exploring the interactions between various ingredients. Extender formulations play a significant role in the standard cryopreservation protocol, and each ingredient has a specific function such as cryoprotectant, antioxidant, energy metabolism function, and membrane function during freeze-thaw [ 3 ]. We observed multiple interactions between each individual component, and this is not possible to discover with conventional experimentation such as comparing two components at a time [ 9 ].…”
Section: Resultsmentioning
confidence: 99%
“…A block is unidimensional if its rho value exceeds 0.70 [ 18 ]. The structural model specified the block-to-block linkages based on our prior review [ 3 ]. The other blocks don’t explain the exogenous latent variables, but they do explain the endogenous latent variables.…”
Section: Methodsmentioning
confidence: 99%
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