Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by<i>Pseudomonas aeruginosa</i>isolates of pus samples<i>in vitro</i>

Salman, Muhammad; Rizwana, Rizwana; Khan, Hayat Muhammad; Munir, Iqbal; Hamayun, Muhammad; Iqbal, Aquib; Rehman, Abdul; Amin, Khalid; Ahmed, Ghayour; Khan, M.K.R.; Khan, Ajmal; Amin, Fazli

doi:10.1080/21691401.2019.1626864

Cited by 30 publications

(16 citation statements)

References 32 publications

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“…The National Institute of Heart, Blood, and Lung reported that up to 80% of all infections caused by bacterial are related to bio lm formation (52). The results of our study showed that 117 (97.5%) isolates formed bio lm, which was similar to other studies (53). Antibiotic resistance has increased by bio lm formation, and resulted in using a higher concentrations of antibiotics in MDR Pseudomonas aeruginosa isolates infections (54).…”

Section: Discussionsupporting

confidence: 87%

Phenotypic and Genotypic Determination of Resistance to Common Disinfectants Among Strains of Pseudomonas Aeruginosa Producing and Non-Producing Biofilm Isolated From Iran

Bakht

Alizadeh

Rahimi

et al. 2021

Preprint

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Background: One of the most important reasons for human mortality worldwide is Hospital-acquired infections, which can be controlled by efficient use of proper disinfectants for the Hospital settings. The main aim of the present survey was to assess the susceptibility of Pseudomonas aeruginosa producing and non-producing biofilm isolated to the five commonly used Hospital disinfectants, and evaluation of the synergistic effect of selective disinfectants and Ethylene-diamine-tetra acetic acid (EDTA), and the effect of exposure to sub-inhibitory concentrations of Sodium hypochlorite on antimicrobial susceptibility test.Results: The results showed that Sodium hypochlorite 5%, and Ethanol 70% is the most and less potent disinfectants against Pseudomonas aeruginosa, respectively. Clearly, the addition of EDTA increased the efficacy of selected disinfectants significantly. The changes in the antibiotic-resistance profiles after exposure to sub-inhibitory concentrations of disinfectants were observed for different classes of antibiotics. As well as near the all isolates harbored efflux pump genes and produced biofilm. Conclusion: For disinfection of Hospital surfaces and instruments, the mixture of disinfectant and EDTA were the most suitable selection in this study. In our study, it was clear that exposure to sub-inhibitory concentrations of disinfectants results in resistance to antibiotics. Also, strong and intermediate biofilm formers belonged to MDR/XDR strains.

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Section: Discussionsupporting

confidence: 87%

Phenotypic and Genotypic Determination of Resistance to Common Disinfectants Among Strains of Pseudomonas Aeruginosa Producing and Non-Producing Biofilm Isolated From Iran

Bakht

Alizadeh

Rahimi

et al. 2021

Preprint

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“…AgNPs work in a synergic form with tobramycin against P. aeruginosa PA01 biofilm, causing extensive cellular changes, including altered cellular morphology and cytoplasmic clearing, and eliminating biofilm formed by this strain [14]. AgNPs combined with polymyxin B has also presented an increase in its antibiofilm activity against antibiotic-sensitive and MDR P. aeruginosa clinical isolates even in low concentrations, when compared to its action alone [101]. Aztreonam can also work in synergism with AgNPs, reducing P. aeruginosa PA01 biofilm biomass and viability in a dose-dependent manner, as well as reducing biofilm thickness and causing cellular [102].…”

Section: Antibiofilm Activity Of Agnps Against Pseudomonas Aeruginosamentioning

confidence: 99%

Antibacterial and antibiofilm potential of silver nanoparticles against antibiotic-sensitive and multidrug-resistant Pseudomonas aeruginosa strains

et al. 2020

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Due to the severity of infections caused by P. aeruginosa and the limitations in treatment, it is necessary to find new therapeutic alternatives. Thus, the use of silver nanoparticles (AgNPs) is a viable alternative because of their potential actions in the combat of microorganisms, showing efficacy against Gram-positive and Gram-negative bacteria, including multidrug-resistant microorganisms (MDR). In this sense, the aim of this work was to conduct a literature review related to the antibacterial and antibiofilm activity of AgNPs against antibiotic-sensitive and multidrug-resistant Pseudomonas aeruginosa strains. The AgNPs are promising for future applications, which may match the clinical need for effective antibiotic therapy. The size of AgNPs is a crucial element to determine the therapeutic activity of nanoparticles, since smaller particles present a larger surface area of contact with the microorganism, affecting their vital functioning. AgNPs adhere to the cytoplasmic membrane and cell wall of microorganisms, causing disruption, penetrating the cell, interacting with cellular structures and biomolecules, and inducing the generation of reactive oxygen species and free radicals. Studies describe the antimicrobial activity of AgNPs at minimum inhibitory concentration (MIC) between 1 and 200 μg/mL against susceptible and MDR P. aeruginosa strains. These studies have also shown antibiofilm activity through disruption of biofilm structure, and oxidative stress, inhibiting biofilm growth at concentrations between 1 and 600 μg/mL of AgNPs. This study evidences the advance of AgNPs as an antibacterial and antibiofilm agent against Pseudomonas aeruginosa strains, demonstrating to be an extremely promising approach to the development of new antimicrobial systems.

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“…Furthermore, ZnO-NPs considerably enhanced the growth inhibition activity of CST against MDR Acinetobacter and reduced its functional dose compared to unaided CST. In a similar study, Salman et al recommended that the combination of silver NPs and CST (Polymyxin B) resulted in increased biofilm inhibition activity against P. aeruginosa(30). Such synergistic activities between conventional antibiotics and NPs may result from inhibiting the export of antibiotics through efflux pump blockage or antibiotic entrance enhancement into the cell via bacterial membrane damage(29).…”

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confidence: 99%

Effect of Zinc Oxide Nanoparticles on Loaded Antibiotics Against Multidrug-Resistant Acinetobacter spp.

Shokrollahi

Bafroee

Saleh

2021

Avicenna J Clin Microbiol Infect

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Background: Metal oxide nanoparticles (NPs) have shown promising efficacy for combating bacterial resistance due to their antibacterial properties. This research investigated the effect of zinc oxide NPs (ZnO-NPs) on the antibacterial activity of conventional antibiotics including ciprofloxacin (CIP), cefotaxime (CTX), and colistin (CST) against multidrug-resistant Acinetobacter isolates. Methods: The disc diffusion method was performed to detect the pattern of antibiotic resistance in isolates. The synthesized ZnO-NPs via the solvothermal method were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDS). Finally, the broth microdilution technique was conducted to demonstrate the antibacterial activity of CIP, CTX, and CST antibiotics with and without a sub-inhibitory concentration of ZnO-NPs. Results: XRD, EDS, and FESEM results confirmed the crystalline structure of ZnO-NPs, and the average size was 100±58.68 nm. All isolates were discovered to be of multidrug-resistant (MDR) type and fully susceptible to CST. The antibacterial activity of CTX and CIP was restored when combined with a sub-inhibitory level of ZnO-NPs (0.25 mg/L), and the highest activity was obtained at the concentrations of 32 µg/mL CTX and 8 µg/ mL CIP. Eventually, ZnO-NPs showed a synergistic effect on the antibacterial properties of CST against MDR Acinetobacter. Conclusions: This research indicated that the combination of ZnO-NPs with some common antibiotics can be considered as a novel strategy for reducing the spread of antibiotic-resistant bacteria.

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Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced byPseudomonas aeruginosaisolates of pus samplesin vitro

Cited by 30 publications

References 32 publications

Phenotypic and Genotypic Determination of Resistance to Common Disinfectants Among Strains of Pseudomonas Aeruginosa Producing and Non-Producing Biofilm Isolated From Iran

Phenotypic and Genotypic Determination of Resistance to Common Disinfectants Among Strains of Pseudomonas Aeruginosa Producing and Non-Producing Biofilm Isolated From Iran

Antibacterial and antibiofilm potential of silver nanoparticles against antibiotic-sensitive and multidrug-resistant Pseudomonas aeruginosa strains

Effect of Zinc Oxide Nanoparticles on Loaded Antibiotics Against Multidrug-Resistant Acinetobacter spp.

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