Synergistic effect of recombinant human bone morphogenic protein-7 and osteogenic differentiation medium on human bone-marrow-derived mesenchymal stem cells in vitro

Zhi, Lianteng; Chen, Chao; Pang, Xiaoli; Uludağ, Hasan; Jiang, Haochuan

doi:10.1007/s00264-011-1247-1

Cited by 18 publications

(10 citation statements)

References 28 publications

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“…This proofs previously published in vitro findings. 6 SS and PN decreased more sharply in BM1, RIA0.1, and RIA1 compared to BM0 and RIA0. Overall, hRIA-MSC seemed to react more sensitively to stimulation by BMP-7.…”

Section: Discussionmentioning

confidence: 81%

“…Although there is some evidence that BMP-7 stimulates bone formation of hBMSC in vitro, the dose dependent BMP-7 stimulation of hBMSC and hRIA-MSC in vivo is even more interesting and less investigated. 6 Furthermore, the treatment of bone defects is still in need of improvement; a balanced combination of MSC and differentiation factors has not yet been found.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Westhauser

Höllig

Reible

et al. 2016

Journal of Orthopaedics

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Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Westhauser

Höllig

Reible

et al. 2016

Journal of Orthopaedics

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“…Numerous studies have confirmed that MSCs form bone by differentiating into osteoblasts [7,27,85]. In addition, many other studies have confirmed that the recruitment of factors with an adequate amount of MSCs and the micro-environment around the fracture are effective for fracture repair [71,84,88].…”

Section: Msc Homing Depends On the Conditionsmentioning

confidence: 99%

Role of mesenchymal stem cells in bone regeneration and fracture repair: a review

Wang

Gou

et al. 2013

International Orthopaedics (SICOT)

272

188

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Mesenchymal stem cells (MSCs) are non-haematopoietic stromal stem cells that have many sources, such as bone marrow, periosteum, vessel walls, adipose, muscle, tendon, peripheral circulation, umbilical cord blood, skin and dental tissues. They are capable of self-replication and of differentiating into, and contributing to the regeneration of, mesenchymal tissues, such as bone, cartilage, ligament, tendon, muscle and adipose tissue. The homing of MSCs may play an important role in the repair of bone fractures. As a composite material, the formation and growth of bone tissue is a complex process, including molecular, cell and biochemical metabolic changes. The recruitment of factors with an adequate number of MSCs and the micro-environment around the fracture are effective for fracture repair. Several studies have investigated the functional expression of various chemokine receptors, trophic factors and adhesion molecules in human MSCs. Many external factors affect MSC homing. MSCs have been used as seed cells in building tissue-engineered bone grafts. Scaffolds seeded with MSCs are most often used in tissue engineering and include biotic and abiotic materials. This knowledge provides a platform for the development of novel therapies for bone regeneration with endogenous MSCs.

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“…In this study, we built 10 constructs from hMSCs of five different patients and added 1 μg ml −1 BMP-7 for stimulation of bone formation [ 21 ]. We performed mCT acquisition before implantation (T0) and after explantation (T1) and compared the results of both time points.…”

Section: Discussionmentioning

confidence: 99%

Heidelberg-mCT-Analyzer: a novel method for standardized microcomputed-tomography-guided evaluation of scaffold properties in bone and tissue research

et al. 2015

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Bone tissue engineering and bone scaffold development represent two challenging fields in orthopaedic research. Micro-computed tomography (mCT) allows non-invasive measurement of these scaffolds’ properties in vivo. However, the lack of standardized mCT analysis protocols and, therefore, the protocols’ user-dependency make interpretation of the reported results difficult. To overcome these issues in scaffold research, we introduce the Heidelberg-mCT-Analyzer. For evaluation of our technique, we built 10 bone-inducing scaffolds, which underwent mCT acquisition before ectopic implantation (T0) in mice, and at explantation eight weeks thereafter (T1). The scaffolds’ three-dimensional reconstructions were automatically segmented using fuzzy clustering with fully automatic level-setting. The scaffold itself and its pores were then evaluated for T0 and T1. Analysing the scaffolds’ characteristic parameter set with our quantification method showed bone formation over time. We were able to demonstrate that our algorithm obtained the same results for basic scaffold parameters (e.g. scaffold volume, pore number and pore volume) as other established analysis methods. Furthermore, our algorithm was able to analyse more complex parameters, such as pore size range, tissue mineral density and scaffold surface. Our imaging and post-processing strategy enables standardized and user-independent analysis of scaffold properties, and therefore is able to improve the quantitative evaluations of scaffold-associated bone tissue-engineering projects.

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Synergistic effect of recombinant human bone morphogenic protein-7 and osteogenic differentiation medium on human bone-marrow-derived mesenchymal stem cells in vitro

Cited by 18 publications

References 28 publications

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Role of mesenchymal stem cells in bone regeneration and fracture repair: a review

Heidelberg-mCT-Analyzer: a novel method for standardized microcomputed-tomography-guided evaluation of scaffold properties in bone and tissue research

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