Synergistic effect of proanthocyanidin and CPP-ACFP on remineralization of artificial root caries

Epasinghe, D.J.; Yiu, Cynthia Kar Yung; Burrow, Michael F.

doi:10.1111/adj.12249

Cited by 24 publications

(16 citation statements)

References 54 publications

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“…Although there was no significant difference between H 1 and H 0 of GSE group, H 1 -H 0 and elastic modulus was higher than the control group. This result can be attributed to prevention of demineralization and strengthening of organic matrix by GSE, and corroborated the previous findings [27,[44][45][46]. Cross-links of collagen fibers are affected by the low pH of caries process and demineralization cycles, leading to decreased elastic modulus [47].…”

Section: Plos Onesupporting

confidence: 89%

Effect of silver diamine fluoride and proanthocyanidin on resistance of carious dentin to acid challenges

et al. 2020

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The aim of this study was to evaluate the effect of silver diamine fluoride and grape seed extract on the microstructure and mechanical properties of carious dentin following exposure to acidic challenge. Ninety-eight molars with occlusal caries were used. In the control group the specimens were kept in distilled water. In the GSE group, the specimens were immersed in 6.5% grape seed extract solution for 30 minutes. In the SDF group, the specimens were immersed in 30% SDF solution for 4 minutes. In the GSE+SDF group, the specimens were immersed in 6.5% grape seed extract solution for 30 minutes and then exposed to 30% SDF solution for 4 minutes. All the groups underwent pH cycling model for 8 days. Microhardness measurements were taken at the baseline before surface treatments and after pH cycling. Elastic modulus was measured, after pH cycling. In the control group, the final hardness was significantly lower than the initial hardness (P = 0.001). In the SDF group, the final hardness was significantly higher than the initial hardness (P < 0.001). There was no significant difference between the initial and final hardness values in the GSE and GSE + SDF groups (p = 0.92, p = 0.07). The H 1-H 0 in the SDF group was significantly higher than the other groups (P<0.05). Moreover, elastic modulus of the experimental groups except GSE+SDF group was significantly higher than control. The highest mean elastic modulus was detected in the SDF group (P<0.001). The use of SDF and GSE prior to the acid challenge improved mechanical properties. Microstructural investigation, using scanning electron microscope showed dentin structure protection against acid challenges with SDF treatment and collagen matrix stabilization with GSE treatment. However combined use of these agents was not beneficious.

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Section: Plos Onesupporting

confidence: 89%

Effect of silver diamine fluoride and proanthocyanidin on resistance of carious dentin to acid challenges

et al. 2020

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“…Eight bonded samples in each group underwent a pH cycling regimen at room temperature. The protocol of pH cycling followed that of Epasinghe et al to mimic high caries risk conditions [ 28 ]. Briefly, samples were immersed in a demineralising solution (50 mM acetate, 1.5 mM CaCl 2 , 0.9 mM KH 2 PO 4 ) at pH 5.0 for 16 h, followed by 8 h of immersion in remineralising solution (150 mM KCl, 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 0.9 mM KH 2 PO 4, 1.5 mM CaCl 2 ) at pH 7.0.…”

Section: Methodsmentioning

confidence: 99%

Shear Bond Strength and Remineralisation Effect of a Casein Phosphopeptide-Amorphous Calcium Phosphate-Modified Glass Ionomer Cement on Artificial “Caries-Affected” Dentine

Zhao

Mei

Zhou

et al. 2017

IJMS

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This study investigated the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP)-modified glass ionomer cement (GIC) on shear bond strength (SBS) and remineralisation of artificial “caries-affected” dentine. Human dentine slices were demineralised and allocated to three groups: group 1, conventional GIC; group 2, CPP-ACP-modified GIC; and group 3, resin-modified GIC. The SBS was measured using a universal testing machine (n = 16 per group). Remaining samples (n = 8 per group) were subjected to pH-cycling for 28 days. After pH-cycling, lesion depth and micro-mechanical properties at the sample-bonding interface were investigated using micro-computed tomography (micro-CT) and nano-indentation, respectively. The SBS for groups 1 to 3 were 4.6 ± 1.5 MPa, 4.2 ± 1.1 MPa, and 5.9 ± 1.9 MPa, respectively (p = 0.007; group 1, group 2 < group 3). Lesion depths determined by micro-CT for groups 1 to 3 were 186 ± 8 µm, 149 ± 14 µm, and 178 ± 8 µm, respectively (p < 0.001; group 2 < group 1, group 3). The mean (±SD, standard deviation) nano-hardness values for groups 1 to 3 were 0.85 ± 0.22 GPa, 1.14 ± 0.21 GPa, and 0.81 ± 0.09 GPa, respectively (p = 0.003; group 1, group 3 < group 2). The mean (±SD) elastic moduli for groups 1 to 3 were 1.70 ± 0.33 GPa, 2.35 ± 0.44 GPa, and 1.59 ± 0.13 GPa, respectively (p < 0.001; group 1, group 3 < group 2). The results suggest that the incorporation of CPP-ACP into GIC does not adversely affect the adhesion to artificial caries-affected dentine. Furthermore, CPP-ACP-modified GIC is superior to conventional GIC in promoting dentine remineralisation.

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“…However, the remineralization of dentin is more complex and less effective process due to absence of seeds of mineral crystals and is still a challenge. (21,22) Repair of demineralized collagen fibrils by mineral implementation within the gaps between the collagen fibrils was tried by the application of topical remineralizing agents such as casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) known as MI paste plus TM (16,(23)(24)(25)(26) CPP-ACPF is a remineralizing agent derived from the milk and can promote high degree of remineralization in enamel as well as dentin compared to fluoride gel or fluoride tooth paste. (27) It has a great potential to modulate bioavailability of Ca and P levels by stabilization of ionic super saturation of Ca and P to enhance the remineralization process and can bind to positively and negatively charged ions.…”

Section: Introductionmentioning

confidence: 99%

Impact of Various Resin Adhesive Strategies on Micro Shear Bond Strength to Sound, Caries- Affected and Remineralized Dentin Substrates: An in-Vitro Esem Analysis

Ghallab

Abd-Elaal

Abd-Elkhalik

2017

Egyptian Dental Journal

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Objectives: The aim of this in vitro study was to evaluate the micro shear bond strength of different adhesive system strategies to sound, artificially caries affected and remineralized dentin substrates. Materials and Methods: Sixty bovine permanent incisors were used. The labial enamel was removed and a flat dentin surface was obtained. The dentin specimens were divided into three main groups (sound as a control, artificial caries affected and remineralized dentin groups). Four adhesive systems were applied over the different dentin substrates according to the directions of the manufacturers; (Futurabond M+, Single bond universal (universal adhesives used in self-etch mode, Futurabond DC (Dual-curing self etch bond with nanofillers and Solobond M (Etch and rinse adhesive). After preparation of the 240 micro tubules of resin composite (Filtek TM Z350XT (Flow able restorative), micro shear bond strength test was performed. The results were tabulated and statistically analyzed. Failure mode was detected for all the fractured dentin specimens using the stereomicroscope. ESEM evaluation was done for resin dentin interfaces for all types of adhesives. Results: There was a statistically significant effect of dentin substrate, while the adhesive system type was not. One way ANOVA followed by Tukey HSD post hoc test showed that, the remineralized dentin recorded the highest micro shear bond strength value with Single Bond Universal followed by Futurabond M+. The artificial caries affected dentin showed the highest micro shear bond strength value with Solobond M followed by Futurabond DC, which also recorded the lowest micro shear bond strength result with remineralized dentin. Conclusions: 1-Performance of the tested adhesives strategies was shown to be dentindependant.2-Dentin pretreatment with CPP-ACPF containing paste affects positively the microshear bond strength of Single bond universal and Futurabond M+ universal adhesives when used in self-etch mode.3-The specific chemical formula of the tested resin adhesives might coordinate with the demineralized dentin.

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Synergistic effect of proanthocyanidin and CPP-ACFP on remineralization of artificial root caries

Abstract: Combined use of PA and CPP-ACFP has a synergistic effect on root caries remineralization by enhancing mineral gain and increasing hardness of artificial root caries.

Cited by 24 publications

References 54 publications

Effect of silver diamine fluoride and proanthocyanidin on resistance of carious dentin to acid challenges

Effect of silver diamine fluoride and proanthocyanidin on resistance of carious dentin to acid challenges

Shear Bond Strength and Remineralisation Effect of a Casein Phosphopeptide-Amorphous Calcium Phosphate-Modified Glass Ionomer Cement on Artificial “Caries-Affected” Dentine

Impact of Various Resin Adhesive Strategies on Micro Shear Bond Strength to Sound, Caries- Affected and Remineralized Dentin Substrates: An in-Vitro Esem Analysis

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