Synergistic effect of N-acetyl-D-glucosamine (NAG) on mitogenic, antigenic and allogeneic stimulation of normal human lymphocytes

Komlos, L.; Ben-Efraim, Shlomo; Lewis, N. J.; Hart, Jacob; Halbrecht, I

doi:10.1016/0192-0561(84)90070-5

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…This is in contrast to previous studies showing that acetylated GlcN enhances cell proliferation in mitogen-, antigen-and alloantigenexposed human peripheral blood lymphocytes [28]. The difference between these and our present data could thus reflect differences between the species.…”

Section: Discussioncontrasting

confidence: 99%

“…In the present work, we did not observe any immunemodulating effects of GlcNAc, except for a slight stimulatory effect in primary MLC supplemented with an exogeneous source of IL-2 (Table 1). This is in contrast to previous studies showing that acetylated GlcN enhances cell proliferation in mitogen-, antigen-and alloantigenexposed human peripheral blood lymphocytes [28]. The difference between these and our present data could thus reflect differences between the species.…”

Section: Discussioncontrasting

confidence: 99%

“…Ingested GlcN might beneficially affect cartilage healing processes [1] by inhibition of catabolic cytokines, as suggested in a recent report showing that GlcN inhibits the effect of destructive proinflammatory cytokines on chondrocytes in vitro [12]. It has been demonstrated that both GlcN and GlcNAc can affect T-cell responses directly having suppressing or enhancing effects, respectively, on mitogen-and allogen-induced DNA and protein synthesis in peripheral blood lymphocytes [13,14]. In addition, GlcN was shown to prevent nonspecific and allospecific activation of T cells and dendritic cells (DCs) in vitro [13].…”

Section: Introductionmentioning

confidence: 96%

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Immunobiological Effects of Glucosamine In Vitro

Forchhammer¹,

Thorn²,

Met³

et al. 2003

Scand J Immunol

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Glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) were assayed in vitro for their effects on proliferation, cytotoxicity and cytokine secretion in primary and secondary mixed lymphocyte cultures (MLCs). In addition, we studied the effect of GlcN and GlcNAc on the proliferation of purified CD4 þ T cells exposed to immobilized anti-CD3 antibody. The present data show that GlcN, but not GlcNAc, inhibits CD4 þ T-cell proliferation, the generation of alloreactive cytotoxic T lymphocytes (CTLs) and the secretion of interferon-g (IFN-g) and interleukin-5 (IL-5) in primary MLC. In secondary T helper-2 (Th2)-polarized MLC, GlcN, but not GlcNAc, inhibits IL-4 and IL-5 secretion, whereas no effect was found on IFN-g secretion in Th1-polarized MLC. Dendritic cells treated with GlcN showed a 75-80% decreased capacity for antigen cross-presentation and allostimulation. In cellular bioassays, GlcN was shown to inhibit the stimulatory activity of IL-4 and IL-2, as well as the cytotoxic activity of tumour necrosis factor-a (TNF-a). In conclusion, GlcN suppresses unprimed T-cell responses by interfering with antigen-presenting cell functions and by a direct inhibitory effect on T-cell proliferation. In addition, GlcN inhibits the secretion of cytokines in antigen-stimulated unprimed T cells and primed Th2-polarized cells.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%