Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds

Dorazilová, Jana; Muchová, Johana; Šmerková, Kristýna; Kočiová, Silvia; Diviš, Pavel; Kopel, Pavel; Veselý, Radek; Pavliňáková, Veronika; Adam, Vojtěch; Vojtová, Lucy

doi:10.3390/nano10101971

Cited by 39 publications

(30 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to our previous studies [ 6 , 9 , 19 ] the FGF2-STAB ® concentration of 2 µg/ml and the SeNPs concentrations of 2, 10 and 20 µg/ml were selected for Collagen/Chitosan scaffold preparation. Table 1 summarizes prepared Collagen/Chitosan sponges for characterization.…”

Section: Resultsmentioning

confidence: 99%

“…This study aims to extend our previous work on collagen-based scaffolds modified with chitosan [ 3 ] and enriched with thermostable fibroblast growth factor 2 (FGF2-STAB ® ) [ 9 ]. Biodegradable collagen/chitosan/FGF2-STAB ® scaffolds revealed promising results in cell culture experiments and have displayed high suitability and biocompatibility to be used as a transferable scaffold for tissue reconstruction.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mutual influence of selenium nanoparticles and FGF2-STAB® on biocompatible properties of collagen/chitosan 3D scaffolds: in vitro and ex ovo evaluation

et al. 2021

Self Cite

View full text Add to dashboard Cite

In a biological system, nanoparticles (NPs) may interact with biomolecules. Specifically, the adsorption of proteins on the nanoparticle surface may influence both the nanoparticles' and proteins' overall bio-reactivity. Nevertheless, our knowledge of the biocompatibility and risk of exposure to nanomaterials is limited. Here, in vitro and ex ovo biocompatibility of naturally based crosslinked freeze-dried 3D porous collagen/chitosan scaffolds, modified with thermostable fibroblast growth factor 2 (FGF2-STAB®), to enhance healing and selenium nanoparticles (SeNPs) to provide antibacterial activity, were evaluated. Biocompatibility and cytotoxicity were tested in vitro using normal human dermal fibroblasts (NHDF) with scaffolds and SeNPs and FGF2-STAB® solutions. Metabolic activity assays indicated an antagonistic effect of SeNPs and FGF2-STAB® at high concentrations of SeNPs. The half-maximal inhibitory concentration (IC50) of SeNPs for NHDF was 18.9 µg/ml and IC80 was 5.6 µg/ml. The angiogenic properties of the scaffolds were monitored ex ovo using a chick chorioallantoic membrane (CAM) assay and the cytotoxicity of SeNPs over IC80 value was confirmed. Furthermore, the positive effect of FGF2-STAB® at very low concentrations (0.01 µg/ml) on NHDF metabolic activity was observed. Based on detailed in vitro testing, the optimal concentrations of additives in the scaffolds were determined, specifically 1 µg/ml of FGF2-STAB® and 1 µg/ml of SeNPs. The scaffolds were further subjected to antimicrobial tests, where an increase in selenium concentration in the collagen/chitosan scaffolds increased the antibacterial activity. This work highlights the antimicrobial ability and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB® and SeNPs. Moreover, we suggest that these sponges could be used as scaffolds for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration. Due to their antimicrobial properties, these scaffolds are also highly promising for tissue replacement requiring the prevention of infection.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mutual influence of selenium nanoparticles and FGF2-STAB® on biocompatible properties of collagen/chitosan 3D scaffolds: in vitro and ex ovo evaluation

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Natural polymers like chitosan, hyaluronic acid (HA), collagen or gelatin and synthetic polymers like polyethyleneglycol (PEG) or polylactic-co-glycolic acid (PLGA) are present in studies related to skin tissue engineering [ 49 , 50 , 51 ]. Chitosan materials filled with selenium nanoparticles were proven to be effective against infections in burnt wounds [ 52 ]. Additionally, scaffolds based on silk fibroin-collagen loaded with titania nanoparticles were adequate for skin tissue regeneration [ 53 ].…”

Section: Polymeric Materials With Antibacterial Activitymentioning

confidence: 99%

Polymeric Materials with Antibacterial Activity: A Review

Olmos

González‐Benito

2021

Polymers

View full text Add to dashboard Cite

Infections caused by bacteria are one of the main causes of mortality in hospitals all over the world. Bacteria can grow on many different surfaces and when this occurs, and bacteria colonize a surface, biofilms are formed. In this context, one of the main concerns is biofilm formation on medical devices such as urinary catheters, cardiac valves, pacemakers or prothesis. The development of bacteria also occurs on materials used for food packaging, wearable electronics or the textile industry. In all these applications polymeric materials are usually present. Research and development of polymer-based antibacterial materials is crucial to avoid the proliferation of bacteria. In this paper, we present a review about polymeric materials with antibacterial materials. The main strategies to produce materials with antibacterial properties are presented, for instance, the incorporation of inorganic particles, micro or nanostructuration of the surfaces and antifouling strategies are considered. The antibacterial mechanism exerted in each case is discussed. Methods of materials preparation are examined, presenting the main advantages or disadvantages of each one based on their potential uses. Finally, a review of the main characterization techniques and methods used to study polymer based antibacterial materials is carried out, including the use of single force cell spectroscopy, contact angle measurements and surface roughness to evaluate the role of the physicochemical properties and the micro or nanostructure in antibacterial behavior of the materials.

show abstract

“…Selenium is one of the important trace elements in the human body that regulates various physiological functions, such as antioxidant behavior, anti-inflammatory effects, and immunity functions [ 36 , 37 , 38 ]. Selenium plays a role in relieving oxidative stress in fibroblasts subjected to heat shock [ 39 ], inhibits the cell cycle, and induces cytotoxicity and apoptosis in carcinogenic cell lines of the colon, breast, lung, or prostate [ 40 , 41 , 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Lee

Patel

et al. 2021

Nanomaterials

View full text Add to dashboard Cite

Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H2O2: 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H2O2 via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were determined, leading to possible applications for bone treatment.

show abstract

Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds

Cited by 39 publications

References 72 publications

Mutual influence of selenium nanoparticles and FGF2-STAB® on biocompatible properties of collagen/chitosan 3D scaffolds: in vitro and ex ovo evaluation

Mutual influence of selenium nanoparticles and FGF2-STAB® on biocompatible properties of collagen/chitosan 3D scaffolds: in vitro and ex ovo evaluation

Polymeric Materials with Antibacterial Activity: A Review

The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Contact Info

Product

Resources

About