Synergistic combination of antioxidants, silver nanoparticles and chitosan in a nanoparticle based formulation: Characterization and cytotoxic effect on MCF-7 breast cancer cell lines

Nayak, Debasis; Minz, Aliva Prity; Ashe, Sarbani; Rauta, Pradipta Ranjan; Kumari, Manisha; Chopra, Pankaj; Nayak, Bismita

doi:10.1016/j.jcis.2016.02.043

Cited by 96 publications

(57 citation statements)

References 39 publications

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“…Our hope for future experiment would be to use a slower growing tumor model, in which ascorbate therapy can be tested over a longer period of time. Another approximation would be to analyze the combination of ascorbic acid with a synergistic compound as in study [94] to evaluate the effect on tumor development and gene expression.…”

Section: Discussionmentioning

confidence: 99%

Gene expression response to ascorbic acid in mice implanted with sarcoma S180 cells

Mikirova¹,

Scimeca²

2016

J Transl Sci

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In recent years, increasing numbers of studies demonstrated that high-dose ascorbate, which can be achieved by intravenous infusion, has cytotoxic effects on cancer cells in vitro and in vivo. There are many hypotheses of anti-cancer mechanisms of high dose ascorbate including a pro-oxidative mechanism, inhibition of angiogenesis and inflammation, enhancement of the anticancer effect of chemotherapy and reduction of chemotherapy-induced side effects. In addition, in recent years there were studies showing that ascorbic acid has effect on gene expression and epigenetic phenomena. In our study we analyzed, by using animal model, the effect of pharmacological concentrations of ascorbic acid on several gene expressions involved in tumorigenesis. To test the effects of ascorbic acid on gene expression, we treated mice with two different concentrations of ascorbic acid after intraperitoneal administration with sarcoma S-180 cells. The injected doses of ascorbic acid were equivalent to 15 g per 70 kg human and 50 g per 70 kg human. Tissue from tumors, liver and kidney was obtained from mice at the end of three weeks of treatment. The gene expression analysis was computed by real time PCR. The results showed significant difference in expression of gene p53 (p<0.02) in tumor tissue between treated and non-treated groups, and reduction of p53 gene expression by size and spreading of tumors. Ascorbate therapy significantly increased expression of NRF2. The experimental data showed that the maximum ascorbate dosage reduced expression of the tumor promoting gene HIF. The dependence of gene expression on the size of tumors was found for P53, HIF and NF-kB. In summary, the results of our study demonstrated that ascorbate therapy had a significant effect on the expression of several genes relevant to the development or inhibition of cancer. Reduced expression of tumor promoting genes as HIF and increased expression of tumor suppression genes such as p53 support the hypothesis that ascorbic acid can act as a potential agent for the suppression of tumor development.

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Section: Discussionmentioning

confidence: 99%

Gene expression response to ascorbic acid in mice implanted with sarcoma S180 cells

Mikirova¹,

Scimeca²

2016

J Transl Sci

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“…The nanoformulations showed high encapsulation efficiency up to 76% with higher antioxidant activity than their base materials and were found to be highly hemocompatible. The nanoformulations also added to the benefits by providing prolonged and sustained drug release only at the targeted site, with minimal side effects to the normal cells [91]. Curcumin encapsulated by nano and pickering emulsion stabilized by chitosan-tripolyphosphate (CS-TPP) nanoparticles were evaluated for their radical scavenging activity and found higher results compared to free curcumin, which dictates the protective effect of the emulsion systems on antioxidant activity of curcumin bioactive compounds [92].…”

Section: Chitosan Originated Nanocarriermentioning

confidence: 99%

Nanoantioxidants: Recent Trends in Antioxidant Delivery Applications

et al. 2019

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Antioxidants interact with free radicals, terminating the adverse chain reactions and converting them to harmless products. Antioxidants thus minimize the oxidative stress and play a crucial role in the treatment of free radicals-induced diseases. However, the effectiveness of natural and/or synthetic antioxidants is limited due to their poor absorption, difficulties to cross the cell membranes, and degradation during delivery, hence contributing to their limited bioavailability. To address these issues, antioxidants covalently linked with nanoparticles, entrapped in nanogel, hollow particles, or encapsulated into nanoparticles of diverse origin have been used to provide better stability, gradual and sustained release, biocompatibility, and targeted delivery of the antioxidants with superior antioxidant profiles. This review aims to critically evaluate the recent scientific evaluations of nanoparticles as the antioxidant delivery vehicles, as well as their contribution in efficient and enhanced antioxidant activities.

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“…CeO 2 is a well-established antioxidant agent in biomedicine [10,11]. The antioxidant properties of CeO 2 can be enhanced when it is combined with certain metals such as gold, silver or platinum [12][13][14][15][16], which have been proposed as biocompatible materials acting as radical scavengers and catalase-like enzymes to treat oxidative stress (OS) [14,[17][18][19][20]. Furthermore, it has been suggested that CeO 2 NPs, alone or in combination with metals, protect against radiation and have beneficial effects on different pathologies such as dementia and infection [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold: An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine

et al. 2020

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Ceria nanoparticles are cell compatible antioxidants whose activity can be enhanced by gold deposition and by surface functionalization with positive triphenylphosphonium units to selectively target the mitochondria. The antioxidant properties of these nanoparticles can serve as the basis of a new strategy for the treatment of several disorders exhibiting oxidative stress, such as cancer, diabetes or Alzheimer’s disease. However, all of these pathologies require a specific antioxidant according with their mechanism to remove oxidant species excess in cells and diminish their effect on mitochondrial function. The mechanism through which ceria nanoparticles neutralize oxidative stress and their effect on mitochondrial function have not been characterized yet. In the present study, the mitochondria antioxidant effect of ceria and ceria-supported gold nanoparticles, with or without triphenylphosphonium functionalization, was assessed in HeLa cells. The effect caused by ceria nanoparticles on mitochondria function in terms of mitochondrial membrane potential (∆Ψm), adenosine triphosphate (ATP) production, nuclear respiratory factor 1 (NRF1) and nuclear factor erythroid–2–like 1 (NFE2L1) was reversed by the presence of gold. Furthermore, this effect was enhanced when nanoparticles were functionalized with triphenylphosphonium. Our study illustrates how the mitochondrial antioxidant effect induced by ceria nanoparticles can be modulated by the presence of gold.

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Synergistic combination of antioxidants, silver nanoparticles and chitosan in a nanoparticle based formulation: Characterization and cytotoxic effect on MCF-7 breast cancer cell lines

Cited by 96 publications

References 39 publications

Gene expression response to ascorbic acid in mice implanted with sarcoma S180 cells

Gene expression response to ascorbic acid in mice implanted with sarcoma S180 cells

Nanoantioxidants: Recent Trends in Antioxidant Delivery Applications

Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold: An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine

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