Synergistic anti-proliferative effect of resveratrol and etoposide on human hepatocellular and colon cancer cell lines

Amiri, Fatemehsadat; Zarnani, Amir‐Hassan; Zand, Hamid; Koohdani, Fariba; Jeddi-Tehrani, Mahmood; Vafa, Mohammadreza

doi:10.1016/j.ejphar.2013.09.020

Cited by 55 publications

(44 citation statements)

References 29 publications

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“…A similar result was also found in HepG2 cells treated with 10 or 20 μg/mL resveratrol for 24h accompanied with an elevation of p21 expression detected by ELISA (41), supporting our previous suppositon. In HepG2 and HCT116 (colon cancer) cells, resveratrol also caused p53 elevation and thus increased apoptosis (42).…”

Section: Apoptosis Led By Cell Cycle Arrest: Importance Of P53 and P21mentioning

confidence: 99%

Anti-tumor effects and cellular mechanisms of resveratrol

Han

Xia

Gao

et al. 2015

DD&T

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Section: Apoptosis Led By Cell Cycle Arrest: Importance Of P53 and P21mentioning

confidence: 99%

Anti-tumor effects and cellular mechanisms of resveratrol

Han

Xia

Gao

et al. 2015

DD&T

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“…It has previously been shown that resveratrol can sensitize HepG2 and HCT-116 (liver and colon cancer cells) to etoposide and augments the anti-proliferative effect of chemotherapeutic drug mitomycin C (MMC) on human colorectal cancer cells [19,20]; therefore, we attempted to determine whether resveratrol could enhance the cytotoxic effects of etoposide in NSCLC cells. The effect of combined treatment with resveratrol and etoposide on cell viability was examined by MTS and trypan blue exclusion assays.…”

Section: Resultsmentioning

confidence: 99%

Resveratrol Enhances Etoposide‐Induced Cytotoxicity through Down‐Regulating ERK1/2 and AKT‐Mediated X‐ray Repair Cross‐Complement Group 1 (XRCC1) Protein Expression in Human Non‐Small‐Cell Lung Cancer Cells

Syu

Chen

et al. 2015

Basic Clin Pharma Tox

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Etoposide (VP-16), a topoisomerase II inhibitor, is an effective anti-cancer drug used for the treatment of non-smallcell lung cancer (NSCLC). Resveratrol is a naturally occurring polyphenolic compound that has been proved to have anti-cancer activity. XRCC1 is an important scaffold protein involved in base excision repair that is regulated by ERK1/2 and AKT signals and plays an important role in the development of lung cancer. However, the role of ERK1/2 and AKT-mediated XRCC1 expression in etoposide treatment alone or combined with resveratrol-induced cytotoxicity in NSCLC cells has not been identified. In this study, etoposide treatment increased XRCC1 mRNA and protein expression through AKT and ERK1/2 activation in two NSCLC cells, H1703 and H1975. Knockdown of XRCC1 in NSCLC cells by transfection of XRCC1 siRNA or inactivation of ERK1/2 and AKT resulted in enhancing cytotoxicity and cell growth inhibition induced by etoposide. Resveratrol inhibited the expression of XRCC1 and enhanced the etoposide-induced cell death and anti-proliferation effect in NSCLC cells. Furthermore, transfection with constitutive active MKK1 or AKT vectors could rescue the XRCC1 protein level and also the cell survival suppressed by co-treatment with etoposide and resveratrol. These findings suggested that down-regulation of XRCC1 expression by resveratrol can enhance the chemosensitivity of etoposide in NSCLC cells.Lung cancer, the leading cause of cancer death in the world, is classified as non-small-cell lung cancer (NSCLC) and small-cell lung cancer [1]. NSCLC accounts for 85% of lung cancer cases, and despite aggressive radio-and/or chemotherapy, fewer than 20% of patients reach a 5-year survival. This poor treatment outcome is due to the primary or acquired drug resistance of NSCLC cells to present cytotoxic therapeutic agents [2,3].Etoposide is an epipodophyllotoxin employed in the therapy of a wide spectrum of cancers [4][5][6]. In vitro studies have shown that etoposide increases topoisomerase II-mediated DNA breakage primarily by inhibiting the ability of the enzyme to religate cleaved nucleic acid molecules [7]. XRCC1 (X-ray repair cross-complementing group 1) is a key mediator of base excision repair (BER). Deficiency of XRCC1 in mice results in embryonic lethality [8,9]. XRCC1 interacts with enzymatic factors such as polyadenosine diphosphate (ADP)-ribose polymerase, DNA ligase III and DNA polymerase b to facilitate efficient repair of DNA single-strand breaks (SSBs) [10]. Down-regulation of XRCC1 expression in human breast cancer cell lines resulted in decreased SSB repair capacity and hypersensitivity to methyl methane sulphonate (MMS) [11]. A previous study has shown that the PI3K-AKT pathway regulates the basal expression of XRCC1 in non-irradiated cells, and MKK1/2-ERK1/2 is essential for the induction of XRCC1 after exposure to radiation [12]. However, whether ERK1/2 and AKT signals involve in regulating XRCC1 expression upon etoposide treatment and its role in the etoposide-induced cytotoxicity in NSCL...

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“…Resveratrol is a natural polyphenolic compound rich in grapes and some other plants (22). Recently, resveratrol was used to manage diabetes and some other chronic diseases.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-B1, Collagen IV and Th17/Treg Related Cytokines in Rats

Zhou

Gao

2014

West Indian Med J

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Objectives: This study aimed at detecting the protective effects of resveratrol on diabetes-induced renal damage and on the expression of transforming growth factor-beta 1 , collagen IV and Th17/Tregrelated and in serum and in the supernatant of the kidney homogenate were determined using ELISA. Results: Diabetic rats had damaged renal function, higher levels of TGF-β1, collagen IV, IL-17A and IL-25, as well as lower levels of IL-35 and IL-10, when compared to the control rats. Compared to the diabetic rats without resveratrol treatment, application of resveratrol to the diabetic rats ameliorated the renal function, inhibited the expression of TGF-β1, collagen IV, IL-17A and IL-25, and increased the expression IL-35 and IL-10. Conclusion: Resveratrol might ameliorate diabetes-induced renal damage through mediating the balance of Th17/Treg-related cytokines and inhibiting the expression of TGF-β1 and collagen IV. Keywords

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Synergistic anti-proliferative effect of resveratrol and etoposide on human hepatocellular and colon cancer cell lines

Cited by 55 publications

References 29 publications

Anti-tumor effects and cellular mechanisms of resveratrol

Anti-tumor effects and cellular mechanisms of resveratrol

Resveratrol Enhances Etoposide‐Induced Cytotoxicity through Down‐Regulating ERK1/2 and AKT‐Mediated X‐ray Repair Cross‐Complement Group 1 (XRCC1) Protein Expression in Human Non‐Small‐Cell Lung Cancer Cells

Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-B1, Collagen IV and Th17/Treg Related Cytokines in Rats

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