Synergistic Activity of Dispersin B and Cefamandole Nafate in Inhibition of Staphylococcal Biofilm Growth on Polyurethanes

Abstract

Antibiotic therapies to eradicate medical device-associated infections often fail because of the ability of sessile bacteria, encased in their exopolysaccharide matrix, to be more drug resistant than planktonic organisms. In the last two decades, several strategies to prevent microbial adhesion and biofilm formation on the surfaces of medical devices, based mainly on the use of antiadhesive, antiseptic, and antibiotic coatings on polymer surfaces, have been developed. More recent alternative approaches are based on molecules able to interfere with quorum-sensing phenomena or to dissolve biofilms. Interestingly, a newly purified ␤-N-acetylglucosaminidase, dispersin B, produced by the gram-negative periodontal pathogen Actinobacillus actinomycetemcomitans, is able to dissolve mature biofilms produced by Staphylococcus epidermidis as well as some other bacterial species. Therefore, in this study, we developed new polymeric matrices able to bind dispersin B either alone or in combination with an antibiotic molecule, cefamandole nafate (CEF). We showed that our functionalized polyurethanes could adsorb a significant amount of dispersin B, which was able to exert its hydrolytic activity against the exopolysaccharide matrix produced by staphylococcal strains. When microbial biofilms were exposed to both dispersin B and CEF, a synergistic action became evident, thus characterizing these polymer-dispersin B-antibiotic systems as promising, highly effective tools for preventing bacterial colonization of medical devices.

Glycoside Hydrolases Degrade Polymicrobial Bacterial Biofilms in Wounds

“…For example, dispersin B is a ␤-hexosaminidase produced by the Gram-negative bacterium Aggregatibacter actinomycetemcomitans in order to disperse adherent cells from a mature biofilm (12). It has been shown that exogenous addition of dispersin B is capable of preventing and disrupting biofilms in vitro and in vivo (13)(14)(15)(16)(17).…”

Creating Smart and Adaptable Medical Devices: Embedding Miniaturized Biosensors

“…Antibiotic coating of medical devices is a commonly deployed technique but has been found to be of limited value, due to a number of factors including poor penetration of the biofilm matrix, transfer of antibiotic resistance via plasmids, high incidence of poly microbial infections, and decreased metabolic state of biofilm bacteria decreasing antibiotic susceptibility [40]. Initial design of antibiotic impregnated medical devices (e.g.…”

Применение Литических Бактериофагов При Хирургическом Лечении Парапротезной Инфекции Эндопротеза Тазобедренного Сустава (Пилотное Исследование)

“…Одним из существующих на сегодняшний день методов борьбы с бактериальными инфекциями, лишенных таких недостатков антибиотиков, как снижение концентрации агента с течением времени и возможные аллергические реакции, являются естественные вирусы, инфицирующие бактерии -бактериофаги [6]. В силу особенностей строения и активности бактериофаги способны преодолевать экзополимерный субстрат биопленок, вызывая значительные изменения в самих биопленках [13] и уничтожать бактерии внутри биопленки, что в случае антибиотиков возможно далеко не для каждого антибиотика.…”