Synergistic Activity for Natural and Synthetic Inhibitors of Angiogenesis Induced by Murine Sarcoma L-1 and Human Kidney Cancer Cells

Abstract

Tumor angiogenesis is an important link in the process of tumor growth and metastasis. A number of substances with an anti-angiogenic activity has been described, but their efficiency remains low. Many researchers believe that a better therapeutic effect could be achieved using a cocktail of several anti-angiogenic agents, having different points of action. A lot of synthetic and natural products of plant and animal origin have anti-tumor and anti-angiogenic properties. The aim of the present study was to evaluate the effect of some combinations of angiogenesis inhibitors on the growth and neovascularization of murine sarcoma L-1 , and on angiogenesis induced in the mouse skin by grafting of human renal cancer. The influence of theobromine, sulindac and its metabolite sulindac sulfone, chlorogenic acid, and shark liver oil on the afferent and efferent angiogenesis pathways was tested. Individually, all of these substances suppressed tumor growth and angiogenesis. Synergy was found for a combination of theobromine, sulindac, and chlorogenic acid (L-1 sarcoma tumor growth), and for theobromine with sulindac sulfone or with shark liver oil, which were given to the mice grafted with human renal cancer cells (angiogenesis). No synergistic effects were shown after preincubation with tumor cells and inhibitors.

Chlorogenic Acid Improves the Regorafenib Effects in Human Hepatocellular Carcinoma Cells

“…Moreover, considering that tumor angiogenesis is an important link in the process of tumor growth and metastasis, a recent study of Balan B.J. and colleagues showed that CGA has anti-angiogenic properties on the neovascularization of murine sarcoma L-1, and on angiogenesis induced in the mouse skin by grafting of human renal cancer [ 42 ].…”

Experimental and Theoretical Study on Theobromine Solubility Enhancement in Binary Aqueous Solutions and Ternary Designed Solvents

“…While theobromine is currently not used as a prescription drug [ 7 ], it attracts the attention of researchers [ 8 , 9 , 10 , 11 , 12 , 13 ] since it possesses several beneficial properties related to human health, such as antioxidant [ 14 ], anti-inflammatory [ 15 ] or immunomodulatory [ 16 ] activities. A very important and widely explored property of theobromine is its anti-cancer activity [ 17 , 18 , 19 , 20 ]. Theobromine may occur in humans as a result of caffeine metabolism in the liver where itself is metabolized into xanthine [ 2 ].…”

Angiogenesis-modulating properties of ethanolic extract of Ferula assa-foetida oleo-gum-resin

“…Different studies suggested that certain natural ingredients may have anti-angiogenesis and antitumour benefits. [20,21] In the present study, the effect of EEFA on angiogenesis-related gene expression is evaluated in hypoxia-induced HUVECs for the 1 st time.…”

“…[19] The combination of multiple anti-angiogenic agents has been considered as the novel approach in the treatment of the diseases. [20] Hence, we aimed to investigate the potency of the oleo-gum-resin cocktail ingredients F. assa-foetida, a native plant to South Khorasan, in targeting angiogenesis. In this study, the anti-angiogenic ability of the ethanolic extract of F. assa-foetida was investigated in human umbilical vein endothelial cells (HUVECs).…”