Synergism of plant-derived polyphenols in adipogenesis: Perspectives and implications

Herranz-López, María; Fernández-Arroyo, Salvador; Pérez-Sánchez, Almudena; Barrajón‐Catalán, Enrique; Beltrán‐Debón, Raúl; Menéndez, Javier A.; Alonso-Villaverde, Carlos; Segura‐Carretero, Antonio; Joven, Jorge; Micol, Vicente

doi:10.1016/j.phymed.2011.12.001

Cited by 125 publications

(136 citation statements)

References 40 publications

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Section: Methodsmentioning

confidence: 99%

“…Hypertrophied adipocytes were obtained using high glucose medium containing insulin (4.5 mg/mL) after differentiation [24]. Under these conditions, cells become hypertrophic adipocytes, a cell model characterized by a high level of cytoplasmic lipid accumulation, insulin-resistance, and exacerbated oxidative stress, a situation reasonably similar to that of obese adipose tissue [16,24]. On day 18, cells were treated with the respective extract or fractions, which were dissolved in 10% FBS/high glucose (4.5 mg/mL) DMEM medium with 1 μM insulin and maintained for 48 h. For cell treatments, extract or fractions were reconstituted in cell culture medium plus DMSO at a maximum final concentration of 0.3% v/v after solvent evaporation.…”

Section: Methodsmentioning

confidence: 99%

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AMPK modulatory activity of olive–tree leaves phenolic compounds: Bioassay-guided isolation on adipocyte model and in silico approach

et al. 2017

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ScopeOlive-tree polyphenols have demonstrated potential for the management of obesity-related pathologies. We aimed to explore the capacity of Olive-tree leaves extract to modulate triglyceride accumulation and AMP-activated protein kinase activity (AMPK) on a hypertrophic adipocyte model.MethodsIntracellular triglycerides and AMPK activity were measured on the hypertrophic 3T3-L1 adipocyte model by AdipoRed and immunofluorescence microscopy, respectively. Reverse phase high performance liquid chromatography coupled to time-of-flight mass detection with electrospray ionization (RP-HPLC-ESI-TOF/MS) was used for the fractionation of the extract and the identification of the compounds. In-silico molecular docking of the AMPK alpha-2, beta and gamma subunits with the identified compounds was performed.ResultsOlive-tree leaves extract decreased the intracellular lipid accumulation through AMPK-dependent mechanisms in hypertrophic adipocytes. Secoiridoids, cinnamic acids, phenylethanoids and phenylpropanoids, flavonoids and lignans were the candidates predicted to account for this effect. Molecular docking revealed that some compounds may be AMPK-gamma modulators. The modulatory effects of compounds over the alpha and beta AMPK subunits appear to be less probable.ConclusionsOlive-tree leaves polyphenols modulate AMPK activity, which may become a therapeutic aid in the management of obesity-associated disturbances. The natural occurrence of these compounds may have important nutritional implications for the design of functional ingredients.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

AMPK modulatory activity of olive–tree leaves phenolic compounds: Bioassay-guided isolation on adipocyte model and in silico approach

et al. 2017

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“…ladanifer and C . salviifoliu s) and Hibiscus sabdariffa in detail [11, 12, 20, 25]. The composition of Lippia citriodora and Hypoxis rooperi extracts has also been the subject of comprehensive studies reported by our group [14, 26, 27].…”

Section: Resultsmentioning

confidence: 99%

“…ladanifer , the extract was obtained by direct aqueous extraction and latter affinity chromatography was performed as previously described [11]. Hibiscus arnottianus specimens were collected from the coast of Granada (37°06'19.0"N 3°33'44.0"W) (Spain) and extracted as reported [20]. Permissions were obtained from local authorities (Conselleria de Agricultura de la Generalitat Valenciana and Consejería de Agricultura de la Comunidad Autónoma de Castilla La Mancha).…”

Section: Methodsmentioning

confidence: 99%

Validation of the AlamarBlue® Assay as a Fast Screening Method to Determine the Antimicrobial Activity of Botanical Extracts

et al. 2016

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Plant compounds are a potential source of new antimicrobial molecules against a variety of infections. Plant extracts suppose complex phytochemical libraries that may be used for the first stages of the screening process for antimicrobials. However, their large variability and complexity require fast and inexpensive methods that allow a rapid and adequate screening for antimicrobial activity against a variety of bacteria and fungi. In this study, a multi-well plate assay using the AlamarBlue® fluorescent dye was applied to screen for antimicrobial activity of several botanical extracts and the data were correlated with microbial colony forming units (CFU). This correlation was performed for three pathogenic model microorganisms: Escherichia coli (Gram negative bacteria), Staphylococcus aureus (Gram positive bacteria) and for the yeast-like fungi Candida albicans. A total of ten plant extracts from different Mediterranean plants, including several Cistus and Hibiscus species, were successfully tested. HPLC-DAD-ESI-MS/MS analysis was utilized for the characterization of the extracts in order to establish structure-activity correlations. The results show that extracts enriched in ellagitannins and flavonols are promising antibacterial agents against both Gram positive and Gram negative bacteria. In contrast, phenolic acids, anthocyanidins and flavonols may be related to the observed antifungal activity.

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“…What is more, in December 2008, when FDA stated that purified rebaudioside A from Stevia rebaudiana Bertoni can be considered Generally Recognized as Safe (GRAS) and in 2009, France approved rebaudioside A for beverages and food, rebaudioside A has been in wide use to edulcorate beverages and some foods in America [5,6]. Besides the significant advantages as natural sweetener for human beings, especially diabetic, phenylketonuria patients, and obese persons [2,7], stevioside and rebaudioside A show various pharmacological activities, such as antihyperglycemic, insulinotropic, and glucagonostatic effects in the diabetic patients [8,9], hypotensive effects [10,11] and immunologic enhancement [12].…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of steviol glycosides fromStevia rebaudianabertoni by macroporous resin and preparative HPLC

Kuang

Yuan

et al. 2014

Acta Chromatographica

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Summary.In this article, a rapid, novel, and effective method was presented to separate pure steviol glycosides from leaves of Stevia rebaudiana Bertoni, including stevioside, rebaudioside A, and rebaudioside C, using resin-based column chromatography followed by preparative high-performance liquid chromatography under hydrophilic interaction liquid chromatography (HILIC) mode. The separation procedure was first optimized on an analytical HILIC column and then scaled up on a preparative HILIC column using a mobile phase consisting of 83% acetonitrile in water with flow rate of 10 mL⋅min −1 at 25°C. 200 mg of the crude extract containing 68.1% steviol glycosides yielded 79.2 mg stevioside, 9.4 mg rebaudioside C, and 33.7 mg rebaudioside A, with the purities of 97.5%, 96.8%, and 97.2%, respectively. Those products were identified by MS, 1 H NMR, and 13 C NMR.

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Synergism of plant-derived polyphenols in adipogenesis: Perspectives and implications

Cited by 125 publications

References 40 publications

AMPK modulatory activity of olive–tree leaves phenolic compounds: Bioassay-guided isolation on adipocyte model and in silico approach

AMPK modulatory activity of olive–tree leaves phenolic compounds: Bioassay-guided isolation on adipocyte model and in silico approach

Validation of the AlamarBlue® Assay as a Fast Screening Method to Determine the Antimicrobial Activity of Botanical Extracts

Preparative separation of steviol glycosides fromStevia rebaudianabertoni by macroporous resin and preparative HPLC

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