Preparative separation of steviol glycosides fromStevia rebaudianabertoni by macroporous resin and preparative HPLC

Abstract

Summary.In this article, a rapid, novel, and effective method was presented to separate pure steviol glycosides from leaves of Stevia rebaudiana Bertoni, including stevioside, rebaudioside A, and rebaudioside C, using resin-based column chromatography followed by preparative high-performance liquid chromatography under hydrophilic interaction liquid chromatography (HILIC) mode. The separation procedure was first optimized on an analytical HILIC column and then scaled up on a preparative HILIC column using a mobile phase consisting of 83% acetonitrile in water with flow rate of 10 mL⋅min −1 at 25°C. 200 mg of the crude extract containing 68.1% steviol glycosides yielded 79.2 mg stevioside, 9.4 mg rebaudioside C, and 33.7 mg rebaudioside A, with the purities of 97.5%, 96.8%, and 97.2%, respectively. Those products were identified by MS, 1 H NMR, and 13 C NMR.

A well‐defined hierarchically porous metal–organic framework and its application in separation and purification of steviol glycosides

“…In addition, referring to the preparative chromatography for SGs separation [12,[46][47], HP-NH 2 -MIL-53 has the general conditions of chromatographic filler: high specific surface area, high porosity and stability, appropriate particle size, with amino group and reveals reversed-phase mode retention properties (the column used for the analytical HPLC is an amine column). Moreover, according to the currently reported literature on MOF as chromatographic column filler [48][49][50], most MOF-based materials can modify the stationary phase as preparative chromatography such as MOF@SiO 2 core-shell stationary phase [49].…”

Separation and Purification of Ursolic Acid from Cynomorium songaricum Exacts with Macroporous Resins

“…Therefore, there has been a growing interest in employing macroporous resins to separate bioactive components from crude extracts of herbal raw materials. For example, macroporous resins have been successfully used in the enrichment of polysaccharide from Astragalus extracts [19], pedunculoside and syringin from Ilex rotunda Thunb extracts [20], steviol glycosides from Stevia rebaudiana bertoni [21]. However, there is no report on using macroporous resins to enrich and separate ursolic acid from C. songaricum extracts so far.…”