Synergism of cellulase, xylanase, and pectinase on hydrolyzing sugarcane bagasse resulting from different pretreatment technologies

Synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to Trichoderma reesei cellulase on rice straw degradation

“…In addition to the action of hydrolytic enzymes, PET can also further increase the efficiency of the enzyme systems by de-esterification of the pectin component, which acts as the embedded matrix in the cell wall structure. This finding was different to a previous work on hydrolysis of pretreated sugarcane bagasse by the mixture of T. reesei cellulase and pectinase in which no synergism was found between the two enzymes, resulting in no increasing glucose production (Li et al, 2014). Addition of a commercial pectin methyl esterase was reported to increase ethanol yield from fermentation of sugar beet pulp by engineered E. coli KO11 expressing cellobiase and pectate lyase (Edwards et al, 2011) .…”

Facilitating the enzymatic saccharification of pulped bamboo residues by degrading the remained xylan and lignin–carbohydrates complexes

“…It is proposed that an ideal pretreatment is able not only to produce a readily digestible substrate with low content of lignin, but also to recover the maximum amount of original sugars in feedstocks (Li et al, 2014a). In this work, although kraft pulping with conventional EA charge was beneficial for extended delignification and enzymatic saccharification, low level of original carbohydrates were retained in the pretreated samples.…”

Associating cooking additives with sodium hydroxide to pretreat bamboo residues for improving the enzymatic saccharification and monosaccharides production

“…Alkali-based pretreatments help to delignify, to disrupt the structure of lignocellulosic biomass, and to expose the cellulose to enzymatic attack producing high enzymatic saccharification efficiency (Gu et al, 2012;Li et al, 2014). As shown in Fig.…”