Synergism in the transformation of hamster embryo cells treated with formaldehyde and adenovirus

Hatch, George G.; Conklin, Patricia M.; Christensen, Carla C.; Casto, Bruce C.; Nesnow, Stephen

doi:10.1002/em.2860050107

Cited by 13 publications

(3 citation statements)

References 17 publications

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“…After EO treatment, SHE cells were rinsed with complete MDM and inoculated with SA7 virus. Adenovirus transformation procedures have been previously reported [Casto, 1968[Casto, , 1973Hatch et al, 1982bHatch et al, , 1983a. SA7 virus was adsorbed for 3 hr (3-4 X lo7 SA7 plaque-forming units per 60-mm Petri dish containing 5-7 x lo6 cells).…”

Section: Quantitation Of Enhanced Sa7 Virus Transformationmentioning

confidence: 99%

“…Teratogenic effects produced by this compound are weak in mice Wmmel and Labrode, 19791 and negative in rats [Snellings et al, 19791. Methods for treating mammalian cells with gases or vapors of volatilized liquids have been developed in our laboratory. These techniques have allowed the evaluation of the genotoxic effects of chlorinated methanes and ethanes and formaldehyde [Hatch et al, 1982a[Hatch et al, , 1983a using chemical enhancement of virus transformation in primary Syrian hamster embryo (SHE) cells. This study was designed to evaluate the use of this exposure methodology for detecting gene mutation and chemical enhancement of virus transformation utilizing the classical mutagen EO.…”

Section: Introductionmentioning

confidence: 99%

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Mutation and enhanced virus transformation of cultured hamster cells by exposure to gaseous ethylene oxide

et al. 1986

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Ethylene oxide is a classical mutagen and a carcinogen based on evidence from studies in experimental animals. It is widely distributed in industrial, research, hospital, and food environments. In an effort to explore the use of newly developed methods for exposing mammalian cells to gaseous or volatile mutagens/carcinogens, Chinese hamster V79 cells were treated for 2 hr with gaseous ethylene oxide, in sealed treatment chambers, and assayed for survival and mutagenic response by analysis of induced resistance to 6-thioguanine or ouabain. Significant numbers of mutants were produced at both genetic markers by 1,250-7,500 ppm ethylene oxide. Similarly, primary Syrian hamster embryo cells were treated for 2 or 20 hr with gaseous ethylene oxide in sealed treatment chambers and subsequently assayed for survival and increased sensitivity to SA7 virus transformation. Treatment concentrations extended from toxic to several nontoxic concentrations. After 2-hr ethylene oxide treatment at 625-2,500 ppm a significant enhancement of virus transformation was observed. At 20 hr after treatment no enhancement was observed. Treatment of hamster cells with ethylene oxide in both bioassay systems yielded concentration-related, quantitative results.

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Section: Quantitation Of Enhanced Sa7 Virus Transformationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mutation and enhanced virus transformation of cultured hamster cells by exposure to gaseous ethylene oxide

et al. 1986

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“…It has also been shown to induce mutations in bacteria (100-102), yeast (103)(104)(105), Drosophila (106), mammalian cells (97,107) and human cells (108). In vitro evidence of formaldehyde's carcinogenic potential is provided by its ability to transform BALB/c 3T3 mouse cells (97), BHK 21 hamster cells (107) and C2H-1OT1/2 mouse cells (100,109) to enhance the transformation of Syrian hamster embryo cells by SA7 adenovirus (110), and to inhibit DNA repair (111).…”

Section: Lopic 2 Future Studiesmentioning

confidence: 99%

Report on the Consensus Workshop on Formaldehyde

1984

Environ Health Perspect

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Report on the Consensus Workshop on Formaldehyde* The Consensus Workshop on Formaldehyde consisted of bringing together scientists from academia, government, industry and public interest groups to address some important toxicological questions concerning the health effects of formaldehyde. The participants in the workshop, the Executive Panel which coordinated the meeting, and the questions posed, all were chosen through a broadly based nomination process in order to achieve as comprehensive a consensus as possible. The subcommittees considered the toxicological problems associated with formaldehyde in the areas of exposure, epidemiology, carcinogenicity/histology/genotoxicity, immunology/sensitization/irritation, structure activity/biochemistry/metabolism, reproduction/teratology, behavior/neurotoxicity/psychology and risk estimation. Some questions considered included the possible human carcinogenicity of formaldehyde, as well as other human health effects, and the interpretation of pathology induced by formaldehyde. These reports, plus introductory material on the procedures used in setting up the Consensus Workshop are presented here. Additionally, there is included a listing of the data base that was made available to the panel chairmen prior to the meeting and was readily accessible to the participants during their deliberations in the meeting. This data base, since it was computerized, was also capable of being searched for important terms. These materials were supplemented by information brought by the panelists. The workshop has defined the consensus concerning a number of major points in formaldehyde toxicology and has identified a number of major deficits in understanding which are important guides to future research. * Executive Panelists (the workshop's executive panel, whose members provided direction to the Consensus Workshop on Formaldehyde and coordinated this report):

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Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Schechtman

Hatch²,

Anderson³

et al. 1986

Environ. mutagen

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The intralaboratory and interlaboratory reproducibility of a DNA virus (SA7) transformation enhancement assay was investigated using nine carcinogenic and noncarcinogenic compounds representing a variety of chemical classes. By the use of standardized procedures designed to limit assay variables, replicate assay data were collected in two independent laboratories and analyzed for concurrence. The carcinogens, 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene, and N-methyl-N'-nitro-N-nitrosoguanidine yielded reproducible dose-dependent cytotoxicity and positive transformation effects (defined as statistically significant [p less than or equal to 0.05] enhancement of virus transformation at two or more consecutive dose levels) in all experiments in both laboratories. The carcinogens lead chromate, diethylnitrosamine, 4-nitroquinoline-N-oxide, and 2-acetylaminofluorene demonstrated enhancement of SA7 transformation at two or more dose levels in 40-50% of the assays. The noncarcinogenic structural analogs anthracene and pyrene consistently did not produce positive assay responses when tested at dose levels up to the limits of solubility. Good interlaboratory concurrence was demonstrated for these model compounds in the Syrian hamster embryo cell-SA7 assay.

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Synergism in the transformation of hamster embryo cells treated with formaldehyde and adenovirus

Cited by 13 publications

References 17 publications

Mutation and enhanced virus transformation of cultured hamster cells by exposure to gaseous ethylene oxide

Mutation and enhanced virus transformation of cultured hamster cells by exposure to gaseous ethylene oxide

Report on the Consensus Workshop on Formaldehyde

Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

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