Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Schechtman, Leonard M.; Hatch, George G.; Anderson, Tara M.; Putman, Donald L.; Kouri, Richard E.; Cameron, J. W.; Nims, Raymond W.; Spalding, Judson W.; Tennant, R. W.; Lubet, Ronald A.

doi:10.1002/em.2860080403

Cited by 7 publications

(1 citation statement)

References 17 publications

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“…These studies demonstrated a high degree of inter-and intralaboratory reproducibility in the SHE/SA7 assay (4). These studies demonstrated a high degree of inter-and intralaboratory reproducibility in the SHE/SA7 assay (4).…”

Section: Discussionmentioning

confidence: 67%

Chemical enhancement of simian adenovirus SA7 oncogenic transformation of Syrian golden hamster embryo (SHE) cells

Lubet¹,

Schechtman

Kiss³

et al. 1986

Journal of Tissue Culture Methods

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A method for measuring the ability of chemicals to enhance focus formation by the highly oncogenic DNA-containing simian adenovirus SA7 is described. The standard test consists of a} pretreatment of target Syrian hamster embryo cells with chemical test articles, b} subsequent treatment of chemically-exposed or control target cells with SA7 virus, and c} determination of cellular toxicity and focus formation in chemically treated and control plates. This paper outlines the procedures for performing the standard test and additionally describes a} methods for preparing the Syrian hamster embryo cells, b} methods for preparing the SA7 virus pools, c) criteria for assay acceptability, and d} criteria used to assign an overall call (positive, negative, or equivocal) to a compound based on the results of multiple individual assays.

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Section: Discussionmentioning

confidence: 67%

Chemical enhancement of simian adenovirus SA7 oncogenic transformation of Syrian golden hamster embryo (SHE) cells

Lubet¹,

Schechtman

Kiss³

et al. 1986

Journal of Tissue Culture Methods

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Comparison of multiple parameters of rodent carcinogenicity and in vitro genetic toxicity

1986

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The relationship between chemically induced patterns of tumorigenesis in rodents and of in vitro genetic toxicity was evaluated for 73 substances. tumorigenicity patterns were defined according to sex and species effects, the induction of common or uncommon tumors, and benign or malignant tumors. These results and the genetic toxicity results derived from the testing of chemicals under code were compared. Chemicals that induced tumors in both sexes of both rodent species (trans-sex/species carcinogens) were divided into those that showed multiple responses in genetic toxicity assays and those that showed little or no response. Some of the nongenotoxic trans-sex/species carcinogens exhibit properties that do not necessarily fit classification as only tumor promoters and may involve some other mode(s) of action. Those chemicals showing tumorigenicity in only one of the four groups exposed (uni-sex/species carcinogens) generally showed little or no response in genetic toxicity assays. Uni-sex/species carcinogens may be difficult to identify by in vitro assays because of their high tissue specificity. Chemicals that are tumorigenic in both sexes of both species are logically more likely to be tumorigenic in a third species than are those that are tumorigenic in only one sex of the exposed species. Therefore, while positive genetic toxicity test results are not predictive of all carcinogens, a consistent positive response among the multiple endpoints in these assays is more likely to identify chemicals with the potential for trans-sex/species carcinogenesis. Such trans-sex/species carcinogens may have the most direct implication for human health effects.

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Chemical enhancement of SA7 virus transformation of hamster embryo cells: Evaluations by interlaboratory testing of diverse chemicals

Hatch¹,

Anderson²,

Lubet³

et al. 1986

Environ. mutagen

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Twelve chemicals from diverse structural classes were tested under code for their capacity to enhance the transformation of Syrian hamster embryo cells by simian adenovirus SA7 in two independent laboratories. Pretreatment of hamster cells with eight of those chemicals (reserpine, dichlorvos, methapyrilene hydrochloride, benzidine dihydrochloride, diphenylhydantoin, cinnamyl anthranilate, 11-aminoundecanoic acid, and 4,4'-oxydianiline) produced repeatable enhancement of SA7 transformation at two or more consecutive dose levels, which constitutes clear evidence of enhancing activity in this assay. Both toxic and nontoxic doses of each of these chemicals caused enhancement of virus transformation. Two chemicals (2,6-dichloro-p-phenylenediamine and cinnamaldehyde) produced some evidence of enhancing activity (repeatable transformation enhancement at one dose). Dose ranges for cytotoxicity and enhancement of SA7 transformation were similar in both laboratories for all chemicals producing activity. The final two chemicals, chloramphenicol sodium succinate and ethylene thiourea, failed to reproducibly demonstrate either significant cytotoxicity or enhancement of SA7 transformation at concentrations up to 10-20 mM. The test results for these 12 chemicals were combined with the test results for 9 known carcinogens and noncarcinogens in order to evaluate relationships between activity, dose response, and lowest effective enhancing concentration for these compounds, as well as to correlate them with rodent carcinogenesis classifications. The Syrian hamster embryo cell-SA7 system demonstrated reproducible test responses in both intra- and interlaboratory studies and detected 13 out of 15 known rodent carcinogens.

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Analysis of the interlaboratory and intralaboratory reproducibility of the enhancement of simian adenovirus SA7 transformation of syrian hamster embryo cells by model carcinogenic and noncarcinogenic compounds

Cited by 7 publications

References 17 publications

Chemical enhancement of simian adenovirus SA7 oncogenic transformation of Syrian golden hamster embryo (SHE) cells

Chemical enhancement of simian adenovirus SA7 oncogenic transformation of Syrian golden hamster embryo (SHE) cells

Comparison of multiple parameters of rodent carcinogenicity and in vitro genetic toxicity

Chemical enhancement of SA7 virus transformation of hamster embryo cells: Evaluations by interlaboratory testing of diverse chemicals

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