Synergism Between Peyer's Patch Cells and Spleen Cells in Humora Antibody Production

Abstract

Good and his colleagues have suggested that vertebrate immunity may have evolved into a two-component system: a thymus-dependent component primarily regulating cellular immunity, and a bursal-dependent system primarily regulating humoral immunity. These workers proposed that such structures as the tonsils, appendix and Peyer's patches may be the primary or central mammalian analogue of the avian bursa of Fabricius, whose removal causes ablation of humoral antibody responses in birds. They found that extirpation of gut-associated lymphoepithelial tissue in rabbits, followed by irradiation, produced deficiencies of specific antibody and immunoglobulin formation (1–3). We have been interested in defining a possible role for Peyer's patches in humoral immunity. We studied first the immune response of spleen and Peyer's patch cells in 1-month-old C57BL/6 mice stimulated with intraperitoneal (i.p.) injections of 2.5 × 108 sheep red blood cells (SRBC).

Frontiers in inflammatory bowel disease

“…Peyer's patches also contain B cells (about 30% in the adult animal, as demonstrated by surface immunoglobulin markers). However, after either oral or parenteral immunization, Peyer's patches do not contain detectable amounts of antigen (139) and do not possess plaque-forming cells (140,141). Although in vivo immunization by any route does not lead to immunization of Peyer's patches, Stobo reported that stimulation by sheep cells in vitro using the Miscelle-Dutton culture technique resulted in a good primary response with Peyer's-patch cells (140); however, Kagnoff and Campbell (142) have reported that these cells were significantly inferior to spleen ceils.…”