Synergetic Effect of SLN-Curcumin and LDH-5-Fu on SMMC-7721 Liver Cancer Cell Line

Abstract

Curcumin and 5-Fluorouracil (5-Fu) have been reported to have anticancer potentials and show certain synergetic effect on some cancer cell lines. However, the poor bioavailability and rapid metabolism limited their medical application. In this study, we encapsulated curcumin with solid lipid nanoparticles (SLN), 5-Fu with Layered double hydroxides (LDHs) separately and tested its properties and anticancer potentials. SLN-curcumin and LDH-5-Fu were determined to be 100 and 60 nm by Transmission Electron Microscopy detection, and the loading efficiency were 28%±2.5% and 16.7%±1.8%, individually. Furthermore, SLN-curcumin and LDH-5-Fu showed a significantly synergetic effect on SMMC-7721 cell stronger than plain drugs together, of which the Idrug loaded nano-carriers was only 0.315. FACS analysis revealed that the combination of SLN-curcumin and LDH-5-Fu induced 80.1% apoptosis in SMMC-7721 cells, which were 1.7-folds of the sum of the two plain drug loaded carriers. The results demonstrated the significant synergetic anticancer potentials of nano-encapsulated curcumin and 5-Fu, which could be further explored for the treatment of other carcinoma.

Antidepressant effects of curcumin and HU-211 coencapsulated solid lipid nanoparticles against corticosterone-induced cellular and animal models of major depression

“…Cur exhibits various pharmacological activities including antioxidant activities, immunomodulatory activities, and neuroprotective activities. [11][12][13] More recently, it has been found that Cur has antidepressant effects on major depression model both in vitro and in vivo. Cur seems to display its antidepressant role by protecting the function of monoaminergic systems and controlling the release of neurotransmitters including dopamine (DA), noradrenaline (NE), and 5-hydroxyindoleacetic acid (5-HIAA).…”

Progress in nanotechnology-based drug carrier in designing of curcumin nanomedicines for cancer therapy: current state-of-the-art

“…The synergistic effect of CUR-SLN and 5-fluorouracil (LDH-FU) on SMMC-7721 liver cancer cell was studied. SLN-CUR and LDH-5-Fu showed a significant synergistic effect on SMMC-7721 cell stronger than plain drugs together [82].…”

Curcumin: an adjuvant therapeutic remedy for liver cancer

“…[74] Synergistic anti-cancer effects of curcumin has also been demonstrated in conjunction with chemotherapeutic drugs such as doxorubicin and paclitaxel by in vivo animal models, and with cisplatin, 5-FU, and adriamycin by in vitro studies. [75][76][77][78][79] Synergistic effects of curcumin have also been demonstrated in combined treatment with anti-angiogenic agents such as leflunomide and perindopril in in vivo mice models. [80] Thus, to conclude, curcumin has a lot of potential to act as an adjuvant remedy in liver cancer.…”