In this paper, cotton fabrics were washed, bleached and then treated with low-temperature plasma of nitrogen then dyed with direct dyes. Some properties of the samples such as zeta potential, weight loss, crystal intensity, dyeability and washing fastness were investigated. The relative color strength and reflection properties of dyed samples were measured by using reflective spectrophotometry. The morphological changes of cotton samples after nitrogen plasma were studied by scanning electron microscope. Also chemical changes and zeta potential of samples have been investigated by using Fourier Transform Infrared Spectroscopy (FTIR) and zeta sizer respectively. The results show that zeta potential of cotton fabrics influenced by plasma treatment and also dye, plasma treatment cause to improve color strength near 20%.