Synchrotron Radiation and Nanotechnology for Stem Cell Research

Osteogenic potential of dualblocks cultured with human periodontal ligament stem cells: in vitro and synchrotron microtomography study

“…Notably, absorption‐based micro‐CT is not recommended for analysis of the present biomaterials, due to low X‐ray absorption by collagenated scaffolds . Our demonstrative experiments were indeed conducted on single sample‐detector distance, with a phase contrast set‐up and subsequent application of a phase retrieval algorithm based on the TIE.…”

“…However, a major factor hampering such endeavors is that the environment where SCs grow or are seeded has critical but poorly understood effects on their fate . Choosing chemical composition and internal structure of a scaffold are major decisions involving a variety of parameters such as phase composition, porosity, pore size and interconnectivity.…”

“…3D visualization techniques have been demonstrated to achieve a greater understanding. X‐ray computed microtomography (micro‐CT), one of the most common 3D imaging techniques, has been applied to the qualitative and quantitative evaluation of tissue growth under different conditions, including engineered bone and tendon . However, data regarding the application of X‐ray‐based techniques to complex constructs such those referring to early stages of in vitro culture of collagenated scaffolds cultured with SCs are still limited.…”

Micro-CT application for infiltration technology in paedodontics and orthodontics

“…Micro-CT is a non-destructive imaging technique which uses X-rays to create high resolution (few microns) crosssections images through a specimen which can later be reconstructed as a 3D model. In the last decade, micro-CT was intensively used for bone [12,13] and bone tissue engineering [14,15], tooth investigations [16,17] and other medical and technological fields [18,19]. Micro-CT was also used for the analysis of the WSL morphology [20].…”

Imagistic Evaluation of the Orthodontics Interfaces

“…Computed micro tomography (micro-CT) is a non-destructive imaging technique which uses X-rays to create high resolution (few microns) cross-sections images through a specimen which can later be reconstructed as a 3D model. Micro-CT was intensively used for bone [19,20] and bone tissue engineering [21,22], tooth investigations [23,24] and other medical and technological fields [25,28]. Micro-CT is similar to conventional computed tomography (CT) used in medical diagnosis.…”