Osteogenic potential of dualblocks cultured with human periodontal ligament stem cells: <i>in vitro</i> and synchrotron microtomography study

Mănescu, Adrian; Giuliani, Alessandra; Mohammadi, Sara; Tromba, Giuliana; Mazzoni, Serena; Diomede, Francesca; Zini, Nicoletta; Piattelli, Adriano; Trubiani, Oriana

doi:10.1111/jre.12289

Cited by 49 publications

(42 citation statements)

References 42 publications

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“…This finding mismatches with previous results obtained on not xeno-free media [16], where the mineralized clusters were detectable just starting from the second week of culture in both the culture media, and were mainly concentrated in the trabecular portion.…”

Section: Resultscontrasting

confidence: 99%

“…In fact, the phase-contrast X-ray imaging provides a substantially enhanced contrast resolution for both soft and mineralized tissues compared to conventional X-ray tube microCT experimental setups. The level of detail within the tomographic images is sufficiently consistent to allow the discrimination between the different levels of mineralization in engineered bone sites [16]. Indeed, the edges and the interfaces between collagenated and mineralized bone (or between areas at different mineralization degrees) could be clearly discernible because of the beam refraction occurring at the edges between tissues with different refraction indexes.…”

Section: Introductionmentioning

confidence: 99%

“…Results were validated by synchrotron radiation (SR) X-Ray PhC-microCT [16], showing that, also at the 3D level and starting from the second week of culture, newly formed mineralized bone was detected in all the scaffolds, both in basal and differentiating media. The limit of this demonstrative research was linked to the nature of the used (not xeno-free) media; it was not clear if they provided equal or increased performance (in cell proliferation, protein production, etc.)…”

Section: Introductionmentioning

confidence: 99%

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Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography

Mazzoni

Mohammadi

Tromba

et al. 2017

IJMS

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This study was designed to quantitatively demonstrate via three-dimensional (3D) images, through the Synchrotron Radiation Phase-Contrast Microtomography (SR-PhC-MicroCT), the osteoinductive properties of a cortico-cancellous scaffold (Osteobiol Dual Block—DB) cultured with human Periodontal Ligament Stem Cells (hPDLSCs) in xeno-free media. In vitro cultures of hPDLSCs, obtained from alveolar crest and horizontal fibers of the periodontal ligament, were seeded onto DB scaffolds and cultured in xeno-free media for three weeks. 3D images were obtained by SR-PhC-microCT after one and three weeks from culture beginning. MicroCT data were successively processed with a phase-retrieval algorithm based on the Transport of Intensity Equation (TIE). The chosen experimental method, previously demonstratively applied for the 3D characterization of the same constructs in not xeno-free media, quantitatively monitored also in this case the early stages of bone formation in basal and differentiating conditions. Interestingly, it quantitatively showed in the xeno-free environment a significant acceleration of the mineralization process, regardless of the culture (basal/differentiating) medium. This work showed in 3D that the DB guides the osteogenic differentiation of hPDLSCs in xeno-free cultures, in agreement with 2D observations and functional studies previously performed by some of the authors. Indeed, here we fully proved in 3D that expanded hPDLSCs, using xeno-free media formulation, not only provide the basis for Good Manufacturing Practice (preserving the stem cells’ morphological features and their ability to differentiate into mesenchymal lineage) but have to be considered, combined to DB scaffolds, as interesting candidates for potential clinical use in new custom made tissue-engineered constructs.

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Section: Resultscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography

Mazzoni

Mohammadi

Tromba

et al. 2017

IJMS

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“…Finally, the formation of mineralized nodules was observed under a stereomicroscope (SZXl6, Olympus, Japan). The quantification of mineralization was performed as described previously [26]. Briefly, 10% cetylpyridinium chloride was added to each well to dissolve the deposition, and the solution was then collected.…”

Section: Cell Adhesion Proliferation and Mineralizationmentioning

confidence: 99%

Icariin-Loaded TiO₂ Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells

Liu

Chen

et al. 2018

Journal of Nanomaterials

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To explore the effects of icariin on the biocompatibility of dental implants, icariin-(ICA-) loaded TiO 2 nanotubes were fabricated on Ti substrates via anodic oxidation and physical absorption. The surface characteristics of the specimens were monitored by field emission scanning electron microscopy (FE-SEM), X-ray diffractometry (XRD), contact angle measurements (CA), and highpressure liquid chromatography. Additionally, the activities of bone marrow cells, such as cytoskeletal, proliferative activities, mineralization, and osteogenesis-related gene expression on the substrates were investigated in detail. The characterization results demonstrated that ICA-loaded TiO 2 nanotubes were successfully fabricated and the hydrophilicity of these TiO 2 nanotubes was significantly higher than that of the pure Ti groups. The results also showed that ICA-loaded TiO 2 nanotubes might not have enhanced effects on cell proliferation and ALP expression. However, it seemed to significantly promote differentiation of bone marrow cells, demonstrated by enhancing the formation of mineralized nodule and the upregulation of the gene expression such as OC, BSP, OPN, and COL-1. The results indicated that ICA-loaded TiO 2 nanotubes can modulate bioactivity of bone marrow cells, which is promising for potential applications in the orthopedics field.

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“…For Alizarin red quantification, 1 ml of 10% cetylpyridinium chloride was added to each well as described previously. 37 Light absorbance of the extracted dye was determined at 562 nm using a spectrophotometer (NanoDrop Technologies, DE, USA). The experiments were performed 3 times.…”

Section: Osteogenic Differentiation Assaysmentioning

confidence: 99%

IL-7 suppresses osteogenic differentiation of periodontal ligament stem cells through inactivation of mitogen-activated protein kinase pathway

Cao

Fan²,

Yonghe³

et al. 2016

Organogenesis

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ABSTRACT. Periodontal ligament stem cells (PDLSCs) are tissue-specific mesenchymal stem cells (MSCs), having an important role in regenerative therapy for teeth loss. Interleukin-7 (IL-7) is a key cytokine produced by stromal cells including MSCs, and exhibits specific roles for B and T cell development and osteoblasts differentiation of multiple myeloma. However, the effect of IL-7 on osteogenic differentiation of PDLSCs remains unclear. Therefore, in the present study we determined whether IL-7 affects the proliferation and osteogenic differentiation of PDLSCs in vitro and explored the associated signaling pathways for IL-7-mediated cell differentiation. The results demonstrated that the isolated human PDLSCs possessed MSCs features, highly expressing CD90, CD44, CD105, CD29 and CD73, and almost did not expressed CD34, CD45, CD11b, CD14 and CD117. IL-7 could not significantly affect the proliferation of PDLSCs, but it decreased their osteogenic differentiation and inhibited alkaline phosphatase (ALP) activity. The results of quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) and Western blotting exhibited that the expression levels of Runx-2, SP7 and osteocalcin (OCN) were significantly reduced by IL-7. Further studies indicated that IL-7 did not significantly change JNK, ERK1/2 and p38 protein production, but markedly suppressed their phosphorylation levels. These data suggest that IL-7 inhibits the osteogenic differentiation of PDLSCs probably via inactivation of mitogen-activated protein kinase (MAPK) signaling pathway.

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Osteogenic potential of dualblocks cultured with human periodontal ligament stem cells: in vitro and synchrotron microtomography study

Abstract: The chosen method, supported by phase contrast micro-CT analysis, successfully and quantitatively monitored the early stages of bone formation and the rate of the bioscaffold resorption in basal and differentiating culture media.

Cited by 49 publications

References 42 publications

Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography

Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography

Icariin-Loaded TiO₂ Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells

IL-7 suppresses osteogenic differentiation of periodontal ligament stem cells through inactivation of mitogen-activated protein kinase pathway

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Osteogenic potential of dualblocks cultured with human periodontal ligament stem cells: in vitro and synchrotron microtomography study

Abstract: The chosen method, supported by phase contrast micro-CT analysis, successfully and quantitatively monitored the early stages of bone formation and the rate of the bioscaffold resorption in basal and differentiating culture media.

Cited by 49 publications

References 42 publications

Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography

Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography

Icariin-Loaded TiO2 Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells

IL-7 suppresses osteogenic differentiation of periodontal ligament stem cells through inactivation of mitogen-activated protein kinase pathway

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About

Icariin-Loaded TiO₂ Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells