Synchrotron FTIR Microspectroscopy Investigations on Biochemical Changes Occurring in Human Cells Exposed to Proton Beams

Abstract

Fourier transform infrared microspectroscopy using a synchrotron radiation source (SR-μFTIR) has great potential in the study of the ionizing radiation effects of human cells by analyzing the biochemical changes occurring in cell components. SR-μFTIR spectroscopy has been usefully employed in recent years in some seminal work devoted to shedding light on processes occurring in cells treated by hadron therapy, that is, radiotherapy with charged heavy particles (mainly protons and carbon ions), which is gaining popularity as a cancer treatment modality. These studies are particularly useful for increasing the effectiveness of radiotherapy cancer treatments with charged particles that can offer significant progress in the treatment of deep-seated and/or radioresistant tumors. In this paper, we present a concise revision of these studies together with the basic principles of μFTIR spectroscopy and a brief presentation of the main characteristics of infrared SR sources. From the analysis of the literature regarding the SR-μFTIR spectroscopy investigation on human cells exposed to proton beams, it is clearly shown that changes in DNA, protein, and lipid cell components are evident. In addition, this review points out that the potential offered by SR-μFTIR in investigating the effects induced by charged particle irradiation have not been completely explored. This is a crucial point for the continued improvement of hadron therapy strategies.

Benchmarking classification abilities of novel optical photothermal IR spectroscopy at the single-cell level with bulk FTIR measurements

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Characterization of freeze-dried oxidized human red blood cells for pre-transfusion testing by synchrotron FTIR microspectroscopy live-cell analysis

“…cells suspended in physiological medium. [15][16][17] Herein, we aim to better understand the effects of oxidation and freezedrying/rehydration on the human RBC membrane by determining changes in spectral data generated from synchrotron FTIR microspectroscopy 'live-cell' analysis of single RBCs.…”

Hyperspectral Channel-Modulated Static Birefringent Fourier Transform Imaging Spectropolarimeter with Zoomable Spectral Resolution

“…Hyperspectral Fourier transform imaging spectroscopy (FTIS) enables the simultaneous acquisition of two-dimensional spatial information and one-dimensional spectral information, and has proven to be a critical tool in numerous scientific and industrial fields, including biomedicine [1][2][3], agriculture [4,5], remote sensing [6], and others. In recent years, several types of Fourier transform imaging spectroscopies (FTISs) have been developed, including interferometers based on Michelson interferometers [7][8][9][10], Sagnac interferometers [11][12][13][14][15][16][17], Mach-Zehnder interferometers [18][19][20], and Fabry-Perot interferometers [21,22].…”