Synchrotron Fourier transform infrared microspectroscopy (sFTIRM) analysis of Al-induced Alzheimer's disease in rat brain cortical tissue

Ahmed, Gehan A.-R.; Abbas, Lamyaa; Sherif, Hadeer H.A.; Kamel, Gihan; Khalil, Samar

doi:10.1016/j.saa.2020.118421

Cited by 11 publications

(5 citation statements)

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“…16 Many studies reported the ability of AlCl 3 to alter the membrane phospholipids' structure, function, and ion homeostasis. 15,17 A daily dose of one g of AlCl 3 for ten days changed the molar ratio of cholesterol/phospholipid and induced membrane uidity of cortex synaptosomes in the rat brain. 18 Therefore, antioxidants that can minimize oxidative stress or stimulate the cellular antioxidants could protect against aluminum poisoning.…”

Section: Introductionmentioning

confidence: 99%

Effects of aluminum chloride and coenzyme Q10 on the molecular structure of lipids and the morphology of the brain hippocampus cells

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Effects of aluminum chloride and coenzyme Q10 on the molecular structure of lipids and the morphology of the brain hippocampus cells

et al. 2021

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“…As seen from Figure 6A, the second derivative spectra of proteins exhibit distinct peaks. The peak at around 1684 cm À1 is associated with beta turns, the peak at around 1658 cm À1 is due to unordered alpha helix, the peak at around 1655 cm À1 is assigned to alpha helix, the peak at around 1648 cm À1 is associated to random coil, the peak at 1638 cm À1 is due to native beta sheet, and the peak at 1629 cm À1 is associated with aggregated beta-sheet structures [46,[50][51][52][53]. As seen from Figure 6B, there is a significant reduction in the intensity of turns and a significant increase in the intensity of random coil structures.…”

Section: Resultsmentioning

confidence: 99%

The investigation of the molecular changes during lipopolysaccharide‐induced systemic inflammation on rat hippocampus by using FTIR spectroscopy

Cakmak‐Arslan,

Kaya,

Mamuk

et al. 2024

Journal of Biophotonics

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The aim of this study is to reveal the molecular changes accompanying the neuronal hyper‐excitability during lipopolysaccharide (LPS)‐induced systemic inflammation on rat hippocampus using Fourier transform infrared (FTIR) spectroscopy. For this aim, the body temperature of Wistar albino rats administered LPS or saline was recorded by radiotelemetry. The animals were decapitated when their body temperature began to decrease by 0.5°C after LPS treatment and the hippocampi of them were examined by FTIR spectroscopy. The results indicated that systemic inflammation caused lipid peroxidation, an increase in the amounts of lipids, proteins and nucleic acids, a decrease in membrane order, an increase in membrane dynamics and changes in the secondary structure of proteins. Principal component analysis successfully separated control and LPS‐treated groups. In conclusion, significant structural, compositional and functional alterations occur in the hippocampus during systemic inflammation and these changes may have specific characteristics which can lead to neuronal hyper‐excitability.

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“…Compared with the control brain, much more creatine deposits were found in the brain samples of both AD mice models and patients, suggesting that the elevation of creatine may play a role in AD progression and may serve as a biomarker for AD diagnosis. Due to its function of exploring the chemical change of the brain tissue of AD models or patients, synchrotron FTIRM has been a valuable technique to test potential treatments in AD mouse models, such as Lepidium sativum ( Ahmed et al, 2020 ).…”

Section: Infrared Spectroscopic Imaging (Irsi)mentioning

confidence: 99%

The Progress of Label-Free Optical Imaging in Alzheimer’s Disease Screening and Diagnosis

Raghunathan

et al. 2021

Front. Aging Neurosci.

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As the major neurodegenerative disease of dementia, Alzheimer’s disease (AD) has caused an enormous social and economic burden on society. Currently, AD has neither clear pathogenesis nor effective treatments. Positron emission tomography (PET) and magnetic resonance imaging (MRI) have been verified as potential tools for diagnosing and monitoring Alzheimer’s disease. However, the high costs, low spatial resolution, and long acquisition time limit their broad clinical utilization. The gold standard of AD diagnosis routinely used in research is imaging AD biomarkers with dyes or other reagents, which are unsuitable for in vivo studies owing to their potential toxicity and prolonged and costly process of the U.S. Food and Drug Administration (FDA) approval for human use. Furthermore, these exogenous reagents might bring unwarranted interference to mechanistic studies, causing unreliable results. Several label-free optical imaging techniques, such as infrared spectroscopic imaging (IRSI), Raman spectroscopic imaging (RSI), optical coherence tomography (OCT), autofluorescence imaging (AFI), optical harmonic generation imaging (OHGI), etc., have been developed to circumvent this issue and made it possible to offer an accurate and detailed analysis of AD biomarkers. In this review, we present the emerging label-free optical imaging techniques and their applications in AD, along with their potential and challenges in AD diagnosis.

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Synchrotron Fourier transform infrared microspectroscopy (sFTIRM) analysis of Al-induced Alzheimer's disease in rat brain cortical tissue

Cited by 11 publications

References 46 publications

Effects of aluminum chloride and coenzyme Q10 on the molecular structure of lipids and the morphology of the brain hippocampus cells

Effects of aluminum chloride and coenzyme Q10 on the molecular structure of lipids and the morphology of the brain hippocampus cells

The investigation of the molecular changes during lipopolysaccharide‐induced systemic inflammation on rat hippocampus by using FTIR spectroscopy

The Progress of Label-Free Optical Imaging in Alzheimer’s Disease Screening and Diagnosis

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