Synchrotron-based FTIR microspectroscopy of chili resistance induced by <i>Bacillus subtilis</i> strain D604 against anthracnose disease

Thumanu, Kanjana; Wongchalee, Darawadee; Sompong, Mathukorn; Phansak, Piyaporn; Thạnh, Trần Đức; Namanusart, Weravart; Vechklang, Kunthika; Kaewnum, S.; Buensanteai, Natthiya

doi:10.1080/17429145.2017.1325523

Cited by 14 publications

(21 citation statements)

References 18 publications

(36 reference statements)

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“…The plant’s susceptibility to disease and the pathogen’s mechanism has previously been described. Spectrum variations in epidermis leaf tissue suggested higher integral areas for the C=O ester from polysaccharides (900 to 1200 cm −1 ), as well as pectin, lignin, or lipids (1700 to 1770 cm −1 ) in chili plants pre-treated with B. subtilis strain D604, then inoculated with C. acutatum [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of a Chitooligosaccharide Mechanism against Bacterial Leaf Blight on Rice by In Vitro and In Silico Studies

Siriwong

Thepbandit

Hoang

et al. 2021

IJMS

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This study focuses on a commercial plant elicitor based on chitooligosaccharides (BIG®), which aids in rice plant growth and disease resistance to bacterial leaf blight (BLB). When the pathogen (Xoo) vigorously attacks rice that has suffered yield losses, it can cause damage in up to 20% of the plant. Furthermore, Xoo is a seed-borne pathogen that can survive in rice seeds for an extended period. In this study, when rice seeds were soaked and sprayed with BIG®, there was a significant increase in shoot and root length, as well as plant biomass. Furthermore, BIG®-treated rice plants showed a significant reduction in BLB severity of more than 33%. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) analysis was used to characterize BIG®’s mechanism in the chemical structure of rice leaves. The SR-FTIR results at 1650, 1735, and 1114 cm−1 indicated changes in biochemical components such as pectins, lignins, proteins, and celluloses. These findings demonstrated that commercial BIG® not only increased rice growth but also induced resistance to BLB. The drug’s target enzyme, Xoo 1075 from Xanthomonas oryzae (PDB ID: 5CY8), was analyzed for its interactions with polymer ingredients, specifically chitooligosaccharides, to gain molecular insights down to the atomic level. The results are intriguing, with a strong binding of the chitooligosaccharide polymer with the drug target, revealing 10 hydrogen bonds between the protein and polymer. Overall, the computational analysis supported the experimentally demonstrated strong binding of chitooligosaccharides to the drug target.

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Section: Discussionmentioning

confidence: 99%

Identification of a Chitooligosaccharide Mechanism against Bacterial Leaf Blight on Rice by In Vitro and In Silico Studies

Siriwong

Thepbandit

Hoang

et al. 2021

IJMS

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“…gloeosporioides isolates CG from Kasetsart University. However, the isolates SLC032, SRTLF01, NKSTKBS02, NKSTKBS05, NKSTKLS02, NKSTFBS01 and KBSF01 were 490 bps, a length comparable to that of the reference C. capsici isolate CC(Thumanu et al., ). These findings provide support for the isolation of Colletotrichum isolates from the sampled cassava tissues.…”

Section: Resultsmentioning

confidence: 93%

Morphological and molecular identification of Colletotrichum species associated with cassava anthracnose in Thailand

Sangpueak

Phansak

Buensanteai

2017

Journal of Phytopathology

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The objective of this study was to identify the causal agent of anthracnose disease of cassava in Thailand. The study was carried out by collecting cassava samples with anthracnose symptoms from various planting areas including 10 districts of eight provinces in Thailand. One hundred and thirty‐six Colletotrichum samples were isolated from cassava anthracnose lesions on leaves, petioles and stems. Thirty‐eight single‐spore isolates were subsequently obtained and cultured on half potato dextrose agar for morphological and molecular characterizations. All 38 isolates were pathogenic with varying degrees of virulence when tested on detached leaves of Kasetsart 50, a susceptible cassava cultivar. Based on their growth habit, colony morphology, conidial morphology and the internal transcribed spacer sequences similarity to that of Colletotrichum accessions in the GenBank, one isolate was identified as C. capsici, one as C. lindemuthianum, two as C. aeschynomene, four as C. boninense and 28 C. gloeosporioides species complex. Geographically, the cosmopolitan C. gloeosporioides species complex was found in all regions, but other species were found only in particular regions. This is, so far, the first report of Colletotrichum complex species associated with cassava anthracnose in Thailand.

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“…According to Zhang et al (2015), phospholipids and phosphatidic acid (PA) belonging to the membrane lipid bilayer act as a signaling immunity and link to ROS activity and SA accumulation, whereas Gao et al (2017) reported lipids and lipid metabolites are important in rice plants to protect against LB and rice blast via plant-microbe interactions [ 32 , 33 ]. Furthermore, enzymes such as beta-glucanases and chitinases are components of pathogenesis-related (PR) proteins, which play an important role in protecting plant cells from pathogen infection [ 17 , 32 , 33 , 34 ]. When plants detect an insect, fungal, bacterial, or virus-viroid attack, beta-1,3-glucanase or chitinase activates plant defenses against fungal infection.…”

Section: Discussionmentioning

confidence: 99%

“…The leaves were fixed with Optimal Cutting Temperature compound (Tissue-Trek ® , Torrance, CA, USA), then rapidly cooled in liquid nitrogen. Each frozen sample was transversely cut with a cryostat microtome (Leica 3050 S, Wetzlar, Germany) at 7 microns and placed on 13 × 2 mm infrared-transparent barium fluoridate slides [ 15 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…The determinations were carried out by using the mode of mapping with an aperture size of 10 × 10 μm, 4 cm −1 of spectral resolution, and 64 scans for the background [ 73 , 74 , 75 ]. Spectral derivative and equipment were performed by OPUS 7.2 software (Bruker Ltd., Hanau, Germany) then, data analyzed by cytospec TM software and unscramblerX10.0 software, NJ, USA [ 34 , 75 ].…”

Section: Methodsmentioning

confidence: 99%

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Identification of Salicylic Acid Mechanism against Leaf Blight Disease in Oryza sativa by SR-FTIR Microspectroscopic and Docking Studies

et al. 2021

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The present study was to investigate the application and mechanism of salicylic acid (SA) as SA-Ricemate for the control of leaf blight disease using a Synchrotron Radiation-based Fourier-Transform Infra-Red (SR-FTIR) microspectroscopy and docking studies. After treating rice plants cv. KDML 105 with SA-Ricemate, the leaves were inoculated with Xanthomonas oryzae pv. oryzae, the causal agent of leaf blight, and disease severity were assessed. The leaves were also used to detect changes in endogenous SA content. The results indicated that SA-Ricemate, as an activated compound, reduced disease severity by 60% at three weeks post-inoculation and increased endogenous content by 50%. The SR-FTIR analysis of changes in the mesophyll of leaves (treated and untreated) showed that the groups of lipids, pectins, and proteins amide I and amide II occurred at higher values, and polysaccharides were shown at lower values in treated compared to untreated. Besides, docking studies were used to model a three-dimensional structure for Pathogenesis-related (PR1b) protein and further identify its interaction with SA. The results showed that ASP28, ARG31, LEU32, GLN97, and ALA93 are important residues that have strong hydrogen bonds with SA. The docking results showed that SA has a good interaction, confirming its role in expression.

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Synchrotron-based FTIR microspectroscopy of chili resistance induced by Bacillus subtilis strain D604 against anthracnose disease

Cited by 14 publications

References 18 publications

Identification of a Chitooligosaccharide Mechanism against Bacterial Leaf Blight on Rice by In Vitro and In Silico Studies

Identification of a Chitooligosaccharide Mechanism against Bacterial Leaf Blight on Rice by In Vitro and In Silico Studies

Morphological and molecular identification of Colletotrichum species associated with cassava anthracnose in Thailand

Identification of Salicylic Acid Mechanism against Leaf Blight Disease in Oryza sativa by SR-FTIR Microspectroscopic and Docking Studies

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