Synchronized Orchestration of miR-99b and let-7g Positively Regulates Rotavirus Infection by Modulating Autophagy

Mukhopadhyay, Urbi; Chanda, Shampa; Patra, Upayan; Mukherjee, Arpita; Rana, Santanu; Mukherjee, Anupam; Chawla‐Sarkar, Mamta

doi:10.1038/s41598-018-38473-8

Cited by 19 publications

(24 citation statements)

References 56 publications

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“…In contrast, functionality of miRNA is retained, as miRNA mimics successfully knocked‐down target expression both at early and late hours of RV‐SA11 infection (Figure e,f). This is in agreement with our previous findings, where miRNA mimics preserved their target repression potency in context of RV‐SA11 infection (Chanda, Nandi, & Chawla‐Sarkar, ; Mukhopadhyay et al, ). We also did not find global change of miRNA population characteristic of abrogated miRNA biogenesis in our miRNA microarray data (Chanda et al, ).…”

Section: Discussionsupporting

confidence: 93%

Biphasic regulation of RNA interference during rotavirus infection by modulation of Argonaute2

Mukhopadhyay

Chanda

Patra

et al. 2019

Cellular Microbiology

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RNA interference (RNAi) is an evolutionary ancient innate immune response in plants, nematodes, and arthropods providing natural protection against viral infection. Viruses have also gained counter‐defensive measures by producing virulence determinants called viral‐suppressors‐of‐RNAi (VSRs). Interestingly, in spite of dominance of interferon‐based immunity over RNAi in somatic cells of higher vertebrates, recent reports are accumulating in favour of retention of the antiviral nature of RNAi in mammalian cells. The present study focuses on the modulation of intracellular RNAi during infection with rotavirus (RV), an enteric virus with double‐stranded RNA genome. Intriguingly, a time point‐dependent bimodal regulation of RNAi was observed in RV‐infected cells, where short interfering RNA (siRNA)‐based RNAi was rendered non‐functional during early hours of infection only to be reinstated fully beyond that early infection stage. Subsequent investigations revealed RV nonstructural protein 1 to serve as a putative VSR by associating with and triggering degradation of Argonaute2 (AGO2), the prime effector of siRNA‐mediated RNAi, via ubiquitin–proteasome pathway. The proviral significance of AGO2 degradation was further confirmed when ectopic overexpression of AGO2 significantly reduced RV infection. Cumulatively, the current study presents a unique modulation of host RNAi during RV infection, highlighting the importance of antiviral RNAi in mammalian cells.

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Section: Discussionsupporting

confidence: 93%

Biphasic regulation of RNA interference during rotavirus infection by modulation of Argonaute2

Mukhopadhyay

Chanda

Patra

et al. 2019

Cellular Microbiology

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“…miR-99b modulates the expression of mTOR following RV infection and modulates autophagy for successful RV replication. 145 Molecular assays for detection and accurate quantitation of hepatitis C virus (HCV) RNA have been important for identification and management of the hepatitis C. ATG5, a target gene for miR-130a, significantly upregulated HCV replication and downregulated interferon-stimulated gene expression. And, miR-130a regulates host antiviral response and HCV replication through targeting ATG5 via the ATG5-dependent autophagy pathway.…”

Section: Mirs Regulate Autophagy In Viral Diseasesmentioning

confidence: 99%

“…Rotavirus (RV), the principal etiological agent of viral gastroenteritis in young children, kills over 200 thousand infants each year. miR‐99b modulates the expression of mTOR following RV infection and modulates autophagy for successful RV replication …”

Section: Introductionmentioning

confidence: 99%

MicroRNAs play an essential role in autophagy regulation in various disease phenotypes

et al. 2019

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Autophagy is a highly conserved catabolic process and fundamental biological process in eukaryotic cells. It recycles intracellular components to provide nutrients during starvation and maintains quality control of organelles and proteins. In addition, autophagy is a well‐organized homeostatic cellular process that is responsible for the removal of damaged organelles and intracellular pathogens. Moreover, it also modulates the innate and adaptive immune systems. Micro ribonucleic acids (microRNAs) are a mature class of post‐transcriptional modulators that are widely expressed in tissues and organs. And, it can suppress gene expression by targeting messenger RNAs for translational repression or, at a lesser extent, degradation. Research indicates that microRNAs regulate autophagy through different pathways, playing an essential role in the treatment of various diseases. It is an important regulator of fundamental cellular processes such as proliferation, autophagy, and cell apoptosis. In this review article, we first review the current knowledge of autophagy and the function of microRNAs. Then, we summarize the mechanism of autophagy and the signaling pathways related to autophagy by citing at least the main proteins involved in the different phases of the process. Second, we introduce other members of RNA and report some examples in various pathologies. Finally, we review the current literature regarding microRNA‐based therapies for cancer, atherosclerosis, cardiac disease, tuberculosis, and viral diseases. MicroRNAs can cause autophagy upregulation or downregulation by targeting genes or affecting autophagy‐related signaling pathways. Therefore, the microRNAs have a huge potential in autophagy regulation, and it is the function as diagnostic and prognostic markers.

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“…Four miRNAs overexpressed in cataract patients were known to inhibit autophagy. 37 – 40 The miR-23b directly targeted the 3′-untranslated region of autophagy-related gene 12 mRNA to suppress the activation of neuronal autophagy. 41 A few miRNAs with decreased expression in cataract patients are known to promote autophagy.…”

Section: Discussionmentioning

confidence: 99%

Investigation of MicroRNA Expression in Anterior Lens Capsules of Senile Cataract Patients and MicroRNA Differences According to the Cataract Type

Kim

Lee

Ko³

et al. 2021

Trans. Vis. Sci. Tech.

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Purpose We investigated the microRNAs (miRNAs) expression in the anterior lens capsules of patients with senile cataract and compared it to that in the anterior lens capsules of healthy controls. Moreover, we compared the differences in miRNAs expression according to the types of cataracts. Methods Individual lens epithelium samples were collected from 33 senile patients and 10 controls. The cataract patients were classified into cortical, nuclear, posterior and anterior subcapsular and mixed. The expression of 12 different miRNAs in lens epithelium was measured using real-time polymerase chain reaction and compared between the senile cataract patients and controls. The differences of miRNA levels according to cataract type were analyzed. Results The expression levels of let-7g-5p, miR-23a-3p, miR-23b-3p, and miR-125a-5p were significantly upregulated in patients with senile cataract when compared with those in the control group ( P < 0.05). The expressions of let-7a-5p, let-7d-5p, miR-16-5p and miR-22-3p were significantly downregulated in the senile cataracts ( P < 0.05). Let-7a-5p, let-7d-5p, let-7g-5p and mir-23b-3p had significant difference in expression between nuclear and anterior subcapsular cataracts. Conclusions The eight differentially expressed miRNAs may be involved in the pathogenesis of senile cataract, in particular, related to oxidative stress and autophagy. Translational Relevance We infer that several miRNAs in lens epithelial cells are promising candidate biomarkers of senile cataracts.

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Synchronized Orchestration of miR-99b and let-7g Positively Regulates Rotavirus Infection by Modulating Autophagy

Cited by 19 publications

References 56 publications

Biphasic regulation of RNA interference during rotavirus infection by modulation of Argonaute2

Biphasic regulation of RNA interference during rotavirus infection by modulation of Argonaute2

MicroRNAs play an essential role in autophagy regulation in various disease phenotypes

Investigation of MicroRNA Expression in Anterior Lens Capsules of Senile Cataract Patients and MicroRNA Differences According to the Cataract Type

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