Synchronization of isothermal calorimetry and liquid cultivation identifying the beneficial conditions for producing ethanol by yeast Saccharomyces cerevisiae fermentation

Tsai, Shu-Yao; Hsu, Yi‐Cheng; Shu, Chi‐Min; Lin, Kuei-Hua; Lin, Chun-Ping

doi:10.1007/s10973-020-09629-4

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…In this study, the viable cell sensor was applied into the ethanol fermentation process by S. cerevisiae and the changes of biomass in the process were obtained on-line in real-time through the measurement of capacitance value, so as to recognize the growth and metabolic state of cells. As for ethanol detection, the usual determining method is by HPLC (Li et al 2019 ; Rehman et al 2019 ; Tsai et al 2020 ), which is also a kind of off-line detection method with many disadvantages such as high cost, long detection time and use of organic solvent. Although it has been reported that near-infrared spectroscopy or Raman spectroscopy combined with partial least squares regression could simultaneously monitor substrate and product in ethanol fermentation process (Pinto et al 2016 ; Nascimento et al 2017 ; Legner et al 2019 ; Schalk et al 2017 ), these methods require expensive near-infrared or Raman instruments and need to build corresponding models for different fermentation systems, which has great limitations in real applications.…”

Section: Resultsmentioning

confidence: 99%

Real-time and on-line monitoring of ethanol fermentation process by viable cell sensor and electronic nose

Feng

Tian

Yang

et al. 2021

Bioresour. Bioprocess.

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In this study, introduction of a viable cell sensor and electronic nose into ethanol fermentation was investigated, which could be used in real-time and on-line monitoring of the amount of living cells and product content, respectively. Compared to the conventional off-line biomass determination, the capacitance value exhibited a completely consistent trend with colony forming units, indicating that the capacitance value could reflect the living cells in the fermentation broth. On the other hand, in comparison to the results of off-line determination by high-performance liquid chromatography, the ethanol concentration measured by electronic nose presented an excellent consistency, so as to realize the on-line monitoring during the whole process. On this basis, a dynamic feeding strategy of glucose guided by the changes of living cells and ethanol content was developed. And consequently, the ethanol concentration, productivity and yield were enhanced by 15.4%, 15.9% and 9.0%, respectively. The advanced sensors adopted herein to monitor the key parameters of ethanol fermentation process could be readily extended to an industrial scale and other similar fermentation processes.

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Section: Resultsmentioning

confidence: 99%

Real-time and on-line monitoring of ethanol fermentation process by viable cell sensor and electronic nose

Feng

Tian

Yang

et al. 2021

Bioresour. Bioprocess.

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“…The heat output Q value was obtained by integrating the metabolic power–time ( P–t ) curves using the TAM Assistant software (v2.0.175, date published 8 October 2020) (Newcastle, DE, USA) [ 35 ]. We used the TAM Air software to control the thermostat [ 33 , 34 ]. All 20-mL glass ampoules were cleaned and sterilized in high-pressure steam (0.1 MPa) at 121 °C prior to use.…”

Section: Methodsmentioning

confidence: 99%

“…Here, we compared the n th-order and the general autocatalytic kinetic equation and evaluated inhibition growth for traditional microbial growth of exothermic reaction under isothermal evaluations by ARKS-FK software. Simulations of isothermal kinetic models can be a single-stage reaction or complex multi-stage reactions, such as the generalized autocatalytic reaction, that may consist of several independent, parallel, and consecutive stages [ 34 ]:…”

Section: Methodsmentioning

confidence: 99%

“…It detects the effect of drugs on cell metabolism by continuously measuring the energy changes produced by biological growth [ 31 , 32 ]. We have developed an approach using isothermal microcalorimetry to evaluate liquid-state microbial growth exothermic properties that include the enthalpy of growth [ 33 , 34 ], biological growth curve differences (including lag, logarithmic, stationary, and decline phases), and isothermal liquid-state growth properties. The approach has been applied to the growth, storage, and culture conditions of Lactobacillus plantarum , Lactobacillus casei , and Saccharomyces cerevisiae that include increasing the exothermic properties of fermentation microorganisms, such as the kinetics of reaction, pre-exponential factor, reaction order, activation energy, enthalpy of endothermic reaction, time to maximum rate, and time to the conversion limit.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms by isothermal kinetic simulation

Tsai

Liu

Lin

et al. 2022

J Therm Anal Calorim

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This study involves isothermal kinetic simulation to evaluate the parameters of inhibition conditions for Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) of high-risk pathogens. This is because the new type of the 2019 novel coronavirus (2019-nCoV) is continuously spreading and the importance of public health issues. Environmental disinfection and personal wearing of masks have become important epidemic prevention measures. Selection of concentration kinetics could be estimated best for E. coli and S. aureus of pathogens, 2.74 × 10 4 and 10 5 and 2.44 × 10 4 and 10 5 colony-forming units (CFU mL –1 ), by isothermal micro-calorimeter (TAM Air) tests, respectively. Comparisons were made of different doses of 0–70 ppm (in 20 mL test ampoule) hypochlorous acid treatment for conducting n th-order and autocatalytic reaction simulation to evaluate the inhibition reaction parameters, which determined the autocatalytic kinetic model that was beneficially applied on the E. coli and S. aureus . We developed the inhibition reaction parameters of the pathogens, which included the activation energy ( E a ), the natural logarithm of pre-exponential factor (ln k 0 ), the enthalpy of inhibition microbial growth reaction ( ∆H ), inhibition microbial growth, and the inhibition growth analysis. Overall, we conducted isothermal kinetic simulation to understand the antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms, which will provide reference for public health and medical-related fields for SDG3, and can contribute to ensuring human health and hygiene.

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“…In this study, the viable cell sensor was applied to the ethanol fermentation process by S. cerevisiae and the changes of biomass in the process were obtained on-line in real-time through the measurement of capacitance value, so as to recognize the growth and metabolic state of cells. As for ethanol detection, the usual determining method is by HPLC (Li et al 2019, Rehman et al 2019, Tsai et al 2020, which is also a kind of off-line detection method with many disadvantages such as high cost, long detection time and use of organic solvent. Although it has been reported that near-infrared spectroscopy combined with partial least squares regression could simultaneously monitor substrate and product in ethanol fermentation process (Pinto et al 2016, Nascimento et al 2017, this method requires expensive near-infrared instruments and needs to build corresponding models for different fermentation systems, which has great limitations in real applications.…”

Section: Development Of Glucose Feeding Strategy With the Guidance Ofmentioning

confidence: 99%

Real-time and on-line monitoring of ethanol fermentation process by viable cell sensor and electronic nose

Feng

Tian

Yang

et al. 2021

Preprint

View full text Add to dashboard Cite

In this study, an introduction of viable cell sensor and electronic nose into ethanol fermentation was investigated, which could be used to real-time and on-line monitor the amount of living cells and product content. Compared to the conventional off-line biomass determination, the capacitance value exhibited a completely consistent trend with colony forming units, indicating that the capacitance value could reflect the living cells in the fermentation broth. On the other hand, in comparison to the results of off-line determination by high performance liquid chromatography, the ethanol concentration measured by electronic nose presented an excellent consistency, so as to realize the on-line monitoring during the whole process. On this basis, a dynamic feeding strategy of glucose guided by the changes of living cells and ethanol content was developed. And consequently, the ethanol concentration, productivity and yield enhanced by 15.4%, 15.9% and 9.0%, respectively. The advanced sensors adopted herein to monitor the key parameters of ethanol fermentation process could be readily extended to an industrial scale and other similar fermentation processes.

show abstract

Synchronization of isothermal calorimetry and liquid cultivation identifying the beneficial conditions for producing ethanol by yeast Saccharomyces cerevisiae fermentation

Cited by 6 publications

References 23 publications

Real-time and on-line monitoring of ethanol fermentation process by viable cell sensor and electronic nose

Real-time and on-line monitoring of ethanol fermentation process by viable cell sensor and electronic nose

Antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms by isothermal kinetic simulation

Real-time and on-line monitoring of ethanol fermentation process by viable cell sensor and electronic nose

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