Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD

Abstract

Inflammatory bowel diseases (IBD) are a chronic inflammatory disorders with increasing global incidence. Synbiotic, which is a two-point approach carrying probiotic and prebiotic components in mitigating inflammation in IBD, is thought to be a pragmatic approach owing to the synergistic outcomes. In this study, the impacts of dietary supplementation with probiotic Bacillus coagulans MTCC5856 spores (B. coagulans) and prebiotic whole plant sugar cane fibre (PSCF) was assessed using a murine model of IBD. Eight-week-old C57BL/6 mice were fed a normal chow diet supplemented with either B. coagulans, PSCF or its synbiotic combination. After seven days of supplementation, colitis was induced with dextran sulfate sodium (DSS) in drinking water for seven days during the continuation of the supplemented diets. Synbiotic supplementation ameliorated disease activity index and histological score (−72%, 7.38, respectively), more effectively than either B. coagulans (−47%, 10.1) and PSCF (−53%, 13.0) alone. Synbiotic supplementation also significantly (p < 0.0001) prevented the expression of tight junction proteins and modulated the altered serum IL-1β (−40%), IL-10 (+26%), and C-reactive protein (CRP) (−39%) levels. Synbiotic supplementations also raised the short-chain fatty acids (SCFA) profile more extensively compared to the unsupplemented DSS-control. The synbiotic health outcome effect of the probiotic and prebiotic combinations may be associated with a synergistic direct immune-regulating efficacy of the components, their ability to protect epithelial integrity, stimulation of probiotic spores by the prebiotic fibre, and/or with stimulation of greater levels of fermentation of fibres releasing SCFAs that mediate the reduction in colonic inflammation. Our model findings suggest synbiotic supplementation should be tested in clinical trials.

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases

“…The authors, therefore, projected the co-administration of resistant potato starch with the members of these bacterial groups to enhance the beneficial effects. Similarly, in our previous study [19], B. coagulans spores combined with whole-plant sugar cane fibre, as a synergistic supplement to DSS-colitic mice, augmented the SCFAs levels along the entire colon length. It is inferred that, beyond the caecum, fibre available for fermentation could be limited.…”

Beyond Heat Stress: Intestinal Integrity Disruption and Mechanism-Based Intervention Strategies

“…Accumulating evidence indicates that provision of a combination of nutritional substances is more effective than treatment with a single dietary component. Synbiotics, a combination of probiotics and prebiotics, have been shown to exert synergistic effects in the intestinal tract [62,257]. Microbiota-stabilizing compounds in combination with other nutritional substances can also enhance beneficial health effects and can possibly be used in a broader range of clinical conditions.…”

Mesenchymal Stem Cells and Acelluar Products Attenuate Murine Induced Colitis

“…Dextran sulfate sodium (DSS)-induced colitis has been widely used as an experimental model to study pathogenic mechanisms underlying IBD, and disease severity was assessed by assigning clinical scores by following previously published protocols. (32)(33)(34) In brief, on day 0, 3% DSS (MW 40 kDa; Sigma-Aldrich, St. Louis, MO) was added to the drinking water. Mice were weighed daily and inspected visually for any sign of sickness.…”

“…Previously published literature described that the mice would develop disease on days 3 to 5 (clinical score = 1, scoring charts listed below). (32,34) The delivery of MSCs, EVs or normal saline (PBS control) was performed on day 4. DSS was continued.…”