Synaptophysin Immunoreactivity in the Cochlear Nuclei of Mammals: A Comparative Study

Bartolomé, María Visitación; Ibáñez, M.A.; López-Sánchez, José; Merchán-Pérez, Ángel; Gil-Loyzaga, P.G.

doi:10.1159/000276447

Cited by 9 publications

(3 citation statements)

References 6 publications

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“…Specific immunoreactivity of this product has been observed in several studies (Micheva et al 2010; Aldahmash and Atteya 2011). Synaptophysin is a presynaptic vesicular glycoprotein that is abundant in the CNS (Bartolomé et al 1993;GilLoyzaga et al 1998).…”

Section: Immunohistochemistrymentioning

confidence: 99%

The Structural Development of the Mouse Dorsal Cochlear Nucleus

Mao

Montgomery

Kubke

et al. 2015

JARO

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The dorsal cochlear nucleus (DCN) is a major subdivision of the mammalian cochlear nucleus (CN) that is thought to be involved in sound localization in the vertical plane and in feature extraction of sound stimuli. The main principal cell type (pyramidal cells) integrates auditory and non-auditory inputs, which are considered to be important in performing sound localization tasks. This study aimed to investigate the histological development of the CD-1 mouse DCN, focussing on the postnatal period spanning the onset of hearing (P12). Fluorescent Nissl staining revealed that the three layers of the DCN were identifiable as early as P6 with subsequent expansion of all layers with age. Significant increases in the size of pyramidal and cartwheel cells were observed between birth and P12. Immunohistochemistry showed substantial changes in synaptic distribution during the first two postnatal weeks with subsequent maturation of the presumed mossy fibre terminals. In addition, GFAP immunolabelling identified several glial cell types in the DCN including the observation of putative tanycytes for the first time. Each glial cell type had specific spatial and temporal patterns of maturation with apparent rapid development during the first two postnatal weeks but little change thereafter. The rapid maturation of the structural organization and DCN components prior to the onset of hearing possibly reflects an influence from spontaneous activity originating in the cochlea/auditory nerve. Further refinement of these connections and development of the non-auditory connections may result from the arrival of acoustic input and experience dependent mechanisms.

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Section: Immunohistochemistrymentioning

confidence: 99%

The Structural Development of the Mouse Dorsal Cochlear Nucleus

Mao

Montgomery

Kubke

et al. 2015

JARO

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“…It could also be used as a complementary method to identify and classify the CN synaptic nerve endings by their shape and size, following the classical types: large cup-shaped buttons, round-ovoid buttons and small buttons. But the most relevant finding is that Syp expression exhibits a conspicuous distribution within the CN in mammals [45,46]. In fact, big differences between the ventral cochlear nucleus (VCN) and the dorsal cochlear nucleus (DCN) were found [8].…”

Section: Neuroplasticity and Synaptic Tree Organization And Reorganizmentioning

confidence: 99%

“…In fact, big differences between the ventral cochlear nucleus (VCN) and the dorsal cochlear nucleus (DCN) were found [8]. A regular pattern of large to mid size buttons was found in the VCN surrounding the neuron cells bodies and nerve fibres, while a dense and homogeneous network of very small puncta-shaped buttons appeared at the DCN [45]. Also, neurons of the cochlear nerve root area, between anterior ventral (AVCN) and posterior ventral cochlear nuclei (PVCN), appeared to be surrounded by large nerve endings containing Syp [8].…”

Section: Neuroplasticity and Synaptic Tree Organization And Reorganizmentioning

confidence: 99%

Cellular and molecular bases of neuroplasticity: brainstem effects after cochlear damage

Gil-Loyzaga¹,

Carricondo

Bartolomé³

et al. 2010

Acta Oto-Laryngologica

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After a cochlear lesion or auditory nerve damage, afferent connections from auditory ganglia can be highly altered. This results in a clear reduction of auditory input and an alteration of connectivity of terminals on cochlear nuclei neurons. Such a process could stimulate the reorganization of the neural circuits and neuroplasticity. Cochlea removal has been demonstrated to be a good model in which to analyse brainstem neuroplasticity, particularly with regard to the cochlear nuclei. After cochlea removal three main periods of degeneration and regeneration were observed. Early effects, during the first week post lesion, involved acute degeneration with nerve ending oedema and degeneration. During the second and, probably, the third post lesion weeks, degeneration was still present, even though a limited and diffuse expression of GAP-43 started. Around 1 month post lesion, degeneration at the cochlear nuclei progressively disappeared and a relevant GAP-43 expression was found. We conclude that neuroplasticity leads neurons to modify their activity and/or their synaptic tree as a consequence of animal adaptation to learning and memory. For the human being neuroplasticity is involved in language learning and comprehension, particularly the acquisition of a second language. Neuroplasticity is important for therapeutic strategies, such as hearing aids and cochlear implants.

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