“…Differentiation of iPSCs to excitatory cortical neurons iPSCs were cultured on Geltrex (ThermoFisher Scientific, A1413202) in NutriStem hPSC XF Medium (Biological Industries, 01-0005). Confluent iPSC cultures were differentiated into cortical excitatory neurons using standard protocols [26,28]. The culture media were replenished daily for 7 days using culture media made with 50% N2 medium comprising 485 mL neurobasal medium (Life Technologies, 21103049), 5 mL N-2 supplement (Gibco, 17502001), 5 mL Glutamax (ThermoFisher Scientific, 35050061), and 5 mL penicillin-streptomycin (Gibco 15140122), and 50% B-27 medium comprising 10 mL B-27 supplement (Gibco, 17504044), 480 mL Dulbecco's modified Eagle medium (DMEM) medium (Sigma-Aldrich, D6421), 5 mL Glutamax (ThermoFisher Scientific, 35050061), and 5 mL penicillin-streptomycin (Gibco 15140122) supplemented with 10 μM SB431542 (Sigma-Aldrich, S4317), 1 μM dorsomorphin (Sigma-Aldrich, P5499), and 100 nM LDN193189 (Sigma-Aldrich, SML0599).…”