Symptom recovery is affected by Cucumber mosaic virus coat protein phosphorylation

Nemes, Katalin; Gellért, Ákos; Bóka, Károly; Vági, Pál; Salánki, Katalin

doi:10.1016/j.virol.2019.08.003

Cited by 16 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The induction of antiviral RNA silencing can result in reduced virus titers and mild or no symptoms in the recovered leaves. CMV induces a speci c symptom recovery phenotype, known as cyclic mosaic symptoms in N. tabacum plants 49,50 . In CMV, defects in the RNA silencing activity of the 2b protein were shown to result in symptom recovery 50 .…”

Section: Discussionmentioning

confidence: 99%

Visual tracking of viral infection dynamics reveals the synergistic interactions between cucumber mosaic virus and broad bean wilt virus 2

Kwon

Kim

et al. 2022

Preprint

View full text Add to dashboard Cite

Cucumber mosaic virus (CMV) is one of the most prevalent plant viruses in the world, and causes severe damage to various crops. CMV has been studied as a model RNA virus to better understand viral replication, gene functions, evolution, virion structure, and pathogenicity. However, CMV infection and movement dynamics remain unexplored due to the lack of a stable recombinant virus tagged with a reporter gene. In this study, we generated a CMV infectious cDNA construct tagged with a variant of the flavin-binding LOV photoreceptor (iLOV). The iLOV gene was stably maintained in the CMV genome after more than four weeks of serial passages between plants. Using the iLOV-tagged recombinant CMV, we visualized CMV infection and movement dynamics in living plants in a time course manner. We also examined whether CMV infection dynamics is influenced by co-infection with broad bean wilt virus 2 (BBWV2). Our results revealed that both the viruses infected the same cells, with BBWV2 having a synergistic effect on the dynamics of CMV infection. Our findings suggest that iLOV-tagged CMV is a useful tool for in planta visual tracking of the virus in functional analyses of viral and host genes involved in viral infection and pathogenicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Visual tracking of viral infection dynamics reveals the synergistic interactions between cucumber mosaic virus and broad bean wilt virus 2

Kwon

Kim

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Since viral concentration was similar in virus-infected plants regardless of AMF colonization, we infer that the mycorrhization may interfere with systemic symptoms development but not with viral replication. Indeed, in the case of CMV infection, symptoms recovery, in addition to the viral titer, may also be affected by the phosphorylation state of the viral coat protein [ 49 ]. Moreover, for other viruses, the viral titer is not always directly correlated with symptomatology [ 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato

Miozzi

Vaira

Brilli

et al. 2020

Viruses

View full text Add to dashboard Cite

Tomato plants can establish symbiotic interactions with arbuscular mycorrhizal fungi (AMF) able to promote plant nutrition and prime systemic plant defenses against pathogens attack; the mechanism involved is known as mycorrhiza-induced resistance (MIR). However, studies on the effect of AMF on viral infection, still limited and not conclusive, indicate that AMF colonization may have a detrimental effect on plant defenses against viruses, so that the term “mycorrhiza-induced susceptibility” (MIS) has been proposed for these cases. To expand the case studies to a not yet tested viral family, that is, Bromoviridae, we investigated the effect of the colonization by the AMF Funneliformis mosseae on cucumber mosaic virus (CMV) infection in tomato by phenotypic, physiological, biochemical, and transcriptional analyses. Our results showed that the establishment of a functional AM symbiosis is able to limit symptoms development. Physiological and transcriptomic data highlighted that AMF mitigates the drastic downregulation of photosynthesis-related genes and the reduction of photosynthetic CO2 assimilation rate caused by CMV infection. In parallel, an increase of salicylic acid level and a modulation of reactive oxygen species (ROS)-related genes, toward a limitation of ROS accumulation, was specifically observed in CMV-infected mycorrhizal plants. Overall, our data indicate that the AM symbiosis influences the development of CMV infection in tomato plants and exerts a priming effect able to enhance tolerance to viral infection.

show abstract

“…sRNA and disease recovery: it is never too late Some virus-infected plants are able to recover from the disease at later stages of infection. This outcome is known as symptom recovery and is characterized by emergence of asymptomatic leaves following a systemic symptomatic infection, despite persistence of the virus [58][59][60]. The plant recovery phenotype thus mimics the tolerant phenotype, and disease recovery can be therefore regarded as an inducible form of tolerance [20].…”

Section: Symbiosis and Tolerance: Are They More Similar Than We Think?mentioning

confidence: 99%

Roles of small RNAs in the establishment of tolerant interaction between plants and viruses

Križnik

Baebler

Gruden

2020

Current Opinion in Virology

View full text Add to dashboard Cite

In a tolerant plant-virus interaction, viral multiplication is sustained without substantial effects on plant growth or reproduction. Such interactions are, in natural environments, frequent and sometimes even beneficial for both interactors. Here we compiled evidence showing that small RNAs modulate plant immune responses and growth, hence adjusting its physiology to enable a tolerant interaction. Importantly, the role of small RNAs in tolerant interactions resembles that required for establishment of a mutualistic symbiosis. Tolerance can become a sustainable strategy for breeding for virus resistance as selection pressure for emergence of more aggressive strains is low. Understanding the processes underlying establishment of tolerance is therefore important for the development of future crops.

show abstract

Symptom recovery is affected by Cucumber mosaic virus coat protein phosphorylation

Cited by 16 publications

References 48 publications

Visual tracking of viral infection dynamics reveals the synergistic interactions between cucumber mosaic virus and broad bean wilt virus 2

Visual tracking of viral infection dynamics reveals the synergistic interactions between cucumber mosaic virus and broad bean wilt virus 2

Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato

Roles of small RNAs in the establishment of tolerant interaction between plants and viruses

Contact Info

Product

Resources

About