Symmetric surficial phaseguides: a passive technology to generate wall-less channels by two-dimensional guiding elements

Bunge, Frank; Driesche, Sander van den; Vellekoop, Michael J.

doi:10.1007/s10404-016-1760-z

Cited by 7 publications

(7 citation statements)

References 22 publications

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“…Consequently, the supply to the cells is based solely on diffusion, meaning that any shear stress on the cells is avoided. The integration of the hydrogels is based on the application of surficial phaseguides as shown in previous work [ 29 ]. Here, the melted hydrogel is pulled into the chip by the negative capillary pressure between the hydrophilic surfaces of the glass.…”

Section: Design Aspectsmentioning

confidence: 99%

“…In order to avoid the gel flowing over the phaseguides, the filling should be achieved pressureless and thus by benefiting of the negative capillary pressure between the hydrophilic glass plates. The aspect ratio of the wall-less channel depends on the contact angle of the glass but is always below 1 [ 29 ]. Technically speaking, a reliable filling is achieved if the channel is two to three times wider than high (i.e., aspect ratio 0.5 or 0.33).…”

Section: Design Aspectsmentioning

confidence: 99%

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Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications

Bunge

Driesche

Vellekoop

2017

Sensors

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Lab-on-a-Chip (LoC) applications for the long-term analysis of mammalian cells are still very rare due to the lack of convenient cell cultivation devices. The difficulties are the integration of suitable supply structures, the need of expensive equipment like an incubator and sophisticated pumps as well as the choice of material. The presented device is made out of hard, but non-cytotoxic materials (silicon and glass) and contains two vertical arranged membranes out of hydrogel. The porous membranes are used to separate the culture chamber from two supply channels for gases and nutrients. The cells are fed continuously by diffusion through the membranes without the need of an incubator and low requirements on the supply of medium to the assembly. The diffusion of oxygen is modelled in order to find the optimal dimensions of the chamber. The chip is connected via 3D-printed holders to the macroscopic world. The holders are coated with Parlyene C to ensure that only biocompatible materials are in contact with the culture medium. The experiments with MDCK-cells show the successful seeding inside the chip, culturing and passaging. Consequently, the presented platform is a step towards Lab-on-a-Chip applications that require long-term cultivation of mammalian cells.

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Section: Design Aspectsmentioning

confidence: 99%

Section: Design Aspectsmentioning

confidence: 99%

Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications

Bunge

Driesche

Vellekoop

2017

Sensors

Self Cite

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“…Using an approach similar to Xurography technology [80,81] the film was cut to the required shape with a plotter cutter (Brother ScanNCut CM900). Corners of the guide were given a minimum radius of 1 mm because sharper corners cause liquid to form pools and leak away [64]. In this study, three different liquid guides were made.…”

Section: Methodsmentioning

confidence: 99%

“…The combination was later used by Wood et al [45,46] to form arrays in open sided chambers and similar methods were used by Wiklund [16]. There has been more explorations of capillary bridges in non-acoustic microfluidics, often with flowing systems [64][65][66]. The liquids are stabilised by being pinned either to a surface [66][67][68] or to threads which act as the fluid guides [69].…”

Section: The Acoustofluidic Capillary Bridgementioning

confidence: 99%

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Node formation mechanisms in acoustofluidic capillary bridges

et al. 2022

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Using acoustofluidic channels formed by capillary bridges two models are developed to describe nodes formed by leaky and by evanescent waves. The liquid channel held between a microscope slide (waveguide) and a strip of polystyrene film (fluid guide) avoids solid-sidewall interactions. With this simplification, our experimental and numerical study showed that waves emitted from a single plane surface, interfere and form the nodes without any resonance in the fluid. Both models pay particular attention to tensor elements normal to the solid-liquid interfaces they find that; initially nodes form in the solid and the node pattern is replicated by waves emitted into the fluid from antinodes in the stress. At fluids depths near half an acoustic wavelength, most nodes are formed by leaky waves. In the glass, water-loading reduces node-node separation and forms an overlay type waveguide which aligns the nodes predominantly along the channel. One new practical insight is that node separation can be controlled by water depth. At 0.2 mm water depths (which are smaller than a ¼ wavelength) nodes form from evanescent waves. Here a suspension of yeast cells formed a pattern of small dot-like clumps of cells on the surface of the polystyrene film. We found the same pattern in sound intensity normal, and close, to the waterpolystyrene interface. The capillary bridge channel developed for this study is simple, low-cost, and could be developed for filtration, separation, or patterning of biological species in rapid immuno-sensing applications.

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Microfluidic devices with gold thin film channels for chemical and biomedical applications: a review

Toudeshkchoui

Rabiee

et al. 2019

Biomed Microdevices

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Symmetric surficial phaseguides: a passive technology to generate wall-less channels by two-dimensional guiding elements

Cited by 7 publications

References 22 publications

Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications

Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications

Node formation mechanisms in acoustofluidic capillary bridges

Microfluidic devices with gold thin film channels for chemical and biomedical applications: a review

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