Symbiotic supplementation on the hemato-immunological parameters and survival of the hybrid surubim after challenge withAeromonas hydrophila

Abstract

This study evaluated the effects of dietary supplementation with two symbiotics in the hybrid surubim (Pseudoplatystoma corruscans 9 P. reticulatum) prior to and after challenge with Aeromonas hydrophila. The fish were divided into unsupplemented fish, supplemented fish with Weissella cibaria and inulin, and supplemented fish with Lactobacillus plantarum and inulin. Twenty days after being fed symbiotics, the fish were sampled for immunological, haematological, and microbiological analysis pre-challenge. After this period, they were challenged with Aeromonas hydrophila, and after 96 h the fish were sampled for blood collection and survival. Fish fed W. cibaria and inulin from the pre-challenge showed the highest values of lysozyme activity. Fish fed L. plantarum and inulin presented a higher number of thrombocytes and granular leukocyte PAS + than the other fish. After challenge, fish fed both symbiotics presented an increased red blood cells number. Fish fed L. plantarum and inulin showed an increased total leucocyte count (WBC), neutrophils, and monocytes number as well as total immunoglobulin. The W. cibaria-and inulin-supplemented fish showed improved haematological and immunological parameters and reduced cumulative mortality after the challenge.

Effects of anEnterococcus faecium‐based probiotic on growth performance and health of Pirarucu,Arapaima gigas

“…The health and survival of fish may reflect the improvement in the immune system stimulated by the probiotic bacteria supplied in the diets (Falaye, Emikpe, Ogundipe, & Oyebanji, ; Jesus et al, ). In the present study, probiotic supplementation resulted in an increase in total leucocytes, thrombocytes, neutrophils and monocytes, indicating an immunostimulatory effect, resulting in a higher protection against adverse conditions and pathogens during cultivation as seen in other species (Dias et al, ; Falaye et al, ; Mouriño et al, ; Telli et al, ; Venkatalakshmi & Ebanasar, ). Thrombocytes are cells that act directly in the immune defence of fish participating in coagulation and phagocytic processes (Haugland, Jordal, & Wergeland, ; Li et al, ; Tavares‐Dias, Ono, Pilarski, & Moraes, ).…”

“…The use of lactic acid bacteria as probiotics in fish diets has been reported as an animal benefit promoter, improving growth and disease resistance (Dawood et al, ; Giri et al, ; Jatobá et al, ; Mohammadian et al, ; Mouriño et al, ). However, the present study is the first to report on the potential use of autochthonous E. faecium to modulate intestinal microbiota to improve growth as well as haematological and immunological responses for A. gigas .…”

“…In this context, reports are available indicating the use of probiotics to reduce disease outbreaks by enhancing immunological system (Dias et al, ; Soltani, Ghosh, et al, ; Soltani, Lymbery, Song, & Hosseini‐Shekarabi, ). The use of probiotics is a strategy to increase production of fish, modulating the intestinal microbiota (Mouriño et al, ; Standen et al, ), improving the immune response (Giri, Sukumaran, & Oviya, ; Jatobá Moraes, Rodrigues, Vieira, & Pereira, ; Moraes et al, ; Mouriño et al, ) and improving growth performance (Dias et al, ; Giri et al, ; Hamdan, El‐Sayed, & Mahmoud, ), during diverse stages of growth (Hamdan et al, ; Jha, Bhujel, & Anal, ; Nakandakare et al, ; Ridha & Azad, ).…”

“…Lactic acid bacteria have shown to be promising probiotics with positive results in various aquatic species including Dicentrarchus labrax (Piccolo et al, ), Oreochromis niloticus (Jatobá et al, , ), Labeo rohita (Giri et al, ) and Astyanax bimaculatus (Moraes et al, ). The positive effect is due to their capacity to colonize the gastrointestinal tract, modifying the microbiota of the intestine and inhibiting the growth of other microorganisms, and improving the immune system (Giri et al, ; Jatobá et al, ; Mouriño et al, ; Yamashita et al, ).…”

Characterization of microbiota inArapaima gigasintestine and isolation of potential probiotic bacteria

“…Autochthonous (strains isolated from the fish host) LAB strains are more likely to display the characteristics and properties required to colonize the intestine (Sun et al 2013). The use of autochthonous LAB strains has been demonstrated to modulate the gut microbiome of fish species, often resulting in positive results for fish immunity and disease resistance (Hjelm et al 2004;He et al 2013;Chi et al 2014;Merrifield and Ringø 2014;Mouriño et al 2015).…”