Symbiotic effectiveness and host ranges of indigenous rhizobia nodulating promiscuous soyabean varieties in Zimbabwean soils

Musiyiwa, Kumbirai; Mpepereki, S.; Giller, Ken E.

doi:10.1016/j.soilbio.2004.12.004

Cited by 49 publications

(31 citation statements)

References 14 publications

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“…Rhizobial isolates like PH 119A, PH 213A, PH 312A, PH 401 and PH 527 were ineffective as SE was < 2.0. Musiyiwa et al [30] also reported high level of variability in soybean effectiveness.…”

Section: Discussionmentioning

confidence: 96%

Molecular diversity of native rhizobia trapped by five field pea genotypes in Indian soils

Wadhwa

Dudeja

Yadav

2010

J. Basic Microbiol.

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Five pea cultivars; HFP 4, HVP 3-5, HFP 9426, Jayanti and Hariyal, being grown in CCS Haryana Agricultural University farm were used to isolate native rhizobia. Selected 54 rhizobia, from all cultivars, were authenticated as rhizobia by plant infectivity test. Along with nodulation, symbiotic effectiveness in terms of symbiotic ratios showed wide range of effectiveness of pea rhizobia from 1.11 to 5.0. DNA of all the 54 rhizobia was extracted and amplified by PCR, using ERIC and 16S rDNA primers. Dendrogram based on ERIC profiles of these 54 rhizobia showed the formation of 13 subclusters at 80% level of similarity. Dendrogram based on RFLP of 16S rDNA by three restriction endonucleases; Msp I, Csp 6I and Rsa I; also formed 13 subclusters at 80% level of similarity. However, positioning of subclusters was different from that of ERIC based dendrogram. Majority of the isolates i.e. 64.8% by ERIC profiles and 44.4% by RFLP of 16S rDNA formed one cluster. Isolates from same nodule were not 100% similar. Considering each cluster representing a rhizobial genotype, both techniques used to assess molecular diversity indicated the presence of 13 genotypes of field pea rhizobia in CCS Haryana Agricultural University farm soil. Two pea rhizobial genotypes were able to nodulate all the five pea cultivars. Furthermore, high strain richness index (0.43-0.5) of field pea rhizobia was observed by both the techniques.

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Section: Discussionmentioning

confidence: 96%

Molecular diversity of native rhizobia trapped by five field pea genotypes in Indian soils

Wadhwa

Dudeja

Yadav

2010

J. Basic Microbiol.

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“…It (Mulongoy and Ayanaba 1986) have reported the presence of Bradyrhizobium japonicum in some African soils even though soybean was not commonly grown. Kasasa (1999) and Musiyiwa et al (2005) reported the presence of indigenous rhizobia nodulating promiscuous soybean varieties in many soils in Fig. 2 Phylogenetic relationship between experimental and GenBank reference strains based on aligned sequences of 16S rRNA gene, constructed as unrooted tree using the nearest neighbor-joining method.…”

Section: Discussionmentioning

confidence: 99%

“…The presence in sufficient populations of effective indigenous soil bradyrhizobia can facilitate TGx varieties to derive nitrogen (N) through biological nitrogen fixation (BNF) and to determine whether or not they will respond to added rhizobia or N fertilizer (Turk et al 1993). Success of inoculation depends on several environmental (abiotic) factors such as extremes of pH, high temperature and desiccation as well as biotic stresses (Brockwell et al 1991;Kahindi et al 1997;Zahran 1999;Musiyiwa et al 2005). Studies have indicated that in some soils, as few as ten competitive indigenous bradyrhizobia per gram of soil can act as an efficient barrier to introduced strains (Thies et al 1991a;Abaidoo et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of indigenous Bradyrhizobium nodulating promiscuous soybean [Glycine max (L) Merr.] varieties in Kenya: Impact of phosphorus and lime fertilization in two contrasting sites

et al. 2009

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While soybean is an exotic crop introduced in Kenya early last century, promiscuous (TGx) varieties which nodulate with indigenous rhizobia have only recently been introduced. Since farmers in Kenya generally cannot afford or access fertilizer or inoculants, the identification of effective indigenous Bradyrhizobium strains which nodulate promiscuous soybean could be useful in the development of inoculant strains. Genetic diversity and phylogeny of indigenous Bradyrhizobium strains nodulating seven introduced promiscuous soybean varieties grown in two different sites in Kenya was assayed using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of the 16S-23S rDNA intergenic spacer region and 16S rRNA gene sequencing. PCR-RFLP analysis directly applied on 289 nodules using Msp I distinguished 18 intergenic spacer groups (IGS) I-XVIII. Predominant IGS groups were I, III, II, IV and VI which constituted 43.9%, 24.6%, 8.3% 7.6% and 6.9% respectively of all the analyzed nodules from the two sites while IGS group VII, IX, X, XI, XII, XIV, XVI, XVII, XVIII each constituted 1% or less. The IGS groups were specific to sites and treatments but not varieties. Phylogenetic analysis of the 16S rRNA gene sequences showed that all indigenous strains belong to the genus Bradyrhizobium. Bradyrhizobium elkanii, Bradyrhizobium spp and Bradyrhizobium japonicum related strains were the most predominant and accounted for 37.9%, 34.5%, and 20.7% respectively while B. yuanmigense related accounted for 6.9% of all strains identified in the two combined sites. The diversity identified in

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“…A promiscuous soyabean variety, Magoye, which nodulates freely with indigenous rhizobia (Musiyiwa et al 2005) was used as the test cultivar. Continuous sole crops grown without any amendments were included to provide baseline yields of maize and soyabean.…”

Section: Methodsmentioning

confidence: 99%

Variable grain legume yields, responses to phosphorus and rotational effects on maize across soil fertility gradients on African smallholder farms

Zingore

Murwira²,

Delve³

et al. 2007

Nutr Cycl Agroecosyst

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Promiscuous soyabean varieties have potential to contribute significantly to income generation, food security and soil N budgets on smallholder farms. One of the major factors limiting this potential is farmers' preference to allocate nutrient resources to food security cereal crops on the most fertile fields, leaving grain legumes to grow on residual fertility on infertile fields. Two experiments were conducted to: (i) compare the current farmer practice with targeting manure and single super phosphate (SSP) to soyabean in a three-year rotation cycle on two fields with different soil fertility: an infertile sandy soil and a more fertile clay soil; and (ii) assess the effects of variability of soil fertility within and across farms on productivity of soyabean and groundnut. In the first experiment, soyabean (<0.2 t ha À1 ) and maize yields (<0.7 t ha À1 ) without fertilizer were poor on a degraded sandy soil. Both crops responded poorly to SSP due to deficiency of other nutrients. Manure application significantly increased soyabean and maize yields, led to yield stabilization over three seasons and also significantly increased the proportion of N 2 fixed by soyabean (measured using 15 N natural abundance) from 60% to 83%. On the sandy soil, P was used more efficiently and gross margins were greater when SSP and manure were applied to maize in a maize-soyabean rotation. Soyabean and maize yields without fertilizer inputs were larger on clay soil with moderate fertility (0.4-0.7 t ha À1 and 2.0-2.3 t ha À1 respectively) and were significantly increased by application of SSP and manure. Within rotations, P recovery was higher when manure and SSP were applied to maize (43 and 25%) than when applied to soyabean (20 and 19%). However, application of manure to soyabean on the clay was more profitable than application to maize for individual crops and within rotations. In the second experiment, soyabean and groundnut yields were largest (*1 and *0.8 t ha À1 respectively) on plots closest to homesteads on wealthy farms, which were more fertile due to good past management. Yields were poor (< 0.5 t ha À1 ) on other fields which previously had received little nutrient inputs. Soyabean and groundnut yields correlated well with available P (R 2 = 0.5-0.7) and soil organic C (SOC) contents (R 2 = 0.4-0.6). For smallholder farmers to maximise benefits from legume production they need to focus attention on the more fertile plots, although production should be optimized in relation to maize. Targeting nutrients to maize as currently practiced by farmers was more efficient and economic under poor soil fertility conditions, whilst potential exists to increase income by targeting manure to soyabean on the more fertile soils.

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Symbiotic effectiveness and host ranges of indigenous rhizobia nodulating promiscuous soyabean varieties in Zimbabwean soils

Cited by 49 publications

References 14 publications

Molecular diversity of native rhizobia trapped by five field pea genotypes in Indian soils

Molecular diversity of native rhizobia trapped by five field pea genotypes in Indian soils

Genetic diversity of indigenous Bradyrhizobium nodulating promiscuous soybean [Glycine max (L) Merr.] varieties in Kenya: Impact of phosphorus and lime fertilization in two contrasting sites

Variable grain legume yields, responses to phosphorus and rotational effects on maize across soil fertility gradients on African smallholder farms

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