Symbiotic Bacteria as Potential Agents for Mosquito Control

Kajla, Mayur K.

doi:10.1016/j.pt.2019.07.003

Cited by 10 publications

(11 citation statements)

References 12 publications

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“…Vector control is a method of extreme relevance to minimize the transmission of disease agents by mosquitoes [28,29]. Therefore, limiting the impact of mosquito-borne diseases is an important goal for global public health agencies [26]. For the control of culicids several methods have been used, which result in reduction of population density, reduction of life span, or impediment of contact with the use of repellent compounds [30].…”

Section: Current Vector Control and Their Resistance To Some Compoundsmentioning

confidence: 99%

“…However, due to the high frequency of use of these compounds, several populations of Aedes spp. have become resistant over the years [14,26,29,[36][37][38].…”

Section: Current Vector Control and Their Resistance To Some Compoundsmentioning

confidence: 99%

“…The use of Bti also presented an impact on the prevalence of malaria [57]. Due to its mechanism of action, with release of toxins in the midgut of the larvae, the development of insect resistance can be hampered [26]. Although some authors have already recorded the occurrence of mosquitoes resistant to Bti [58,59].…”

Section: Biological Control Of Vectorsmentioning

confidence: 99%

“…These often-devastating arboviruses seem to continue to affect millions of people worldwide [26]. Therefore, vector control, whether in immature or adult stages, is a crucial strategy for preventing the expansion of these diseases [26,27].…”

Section: Introductionmentioning

confidence: 99%

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The great potential of entomopathogenic bacteria Xenorhabdus and Photorhabdus for mosquito control: a review

et al. 2020

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The control of insects of medical importance, such as Aedes aegypti and Aedes albopictus are still the only effective way to prevent the transmission of diseases, such as dengue, chikungunya and Zika. Their control is performed mainly using chemical products; however, they often have low specificity to non-target organisms, including humans. Also, studies have reported resistance to the most commonly used insecticides, such as the organophosphate and pyrethroids. Biological control is an ecological and sustainable method since it has a slow rate of insect resistance development. Bacterial species of the genera Xenorhabdus and Photorhabdus have been the target of several research groups worldwide, aiming at their use in agricultural, pharmaceutical and industrial products. This review highlights articles referring to the use of Xenorhabdus and Photorhabdus for insects and especially for mosquito control proposing future ways for their biotechnological applicability. Approximately 24 species of Xenorhabdus and five species of Photorhabdus have been described to have insecticidal properties. These studies have shown genes that are capable of encoding low molecular weight proteins, secondary toxin complexes and metabolites with insecticide activities, as well as antibiotic, fungicidal and antiparasitic molecules. In addition, several species of Xenorhabdus and Photorhabdus showed insecticidal properties against mosquitoes. Therefore, these biological agents can be used in new control methods, and must be, urgently considered in short term, in studies and applications, especially in mosquito control.

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Section: Current Vector Control and Their Resistance To Some Compoundsmentioning

confidence: 99%

“…However, due to the high frequency of use of these compounds, several populations of Aedes spp. have become resistant over the years [14,26,29,[36][37][38].…”

Section: Current Vector Control and Their Resistance To Some Compoundsmentioning

confidence: 99%

Section: Biological Control Of Vectorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The great potential of entomopathogenic bacteria Xenorhabdus and Photorhabdus for mosquito control: a review

et al. 2020

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“…Specifically, paratransgenesis studies have illustrated that heterologous expression of antimalarial (Shane et al, 2018; Wang et al, 2017) or insecticidal (Lovett et al, 2019) proteins or peptides in microbiome species can efficiently reduce parasite load or influence mosquito survival, respectively. Thus far, these approaches have primarily employed insect venom proteins as the inhibitory agent, but antimalarial or insecticidal small molecules are also attractive candidates (Kajla, 2019).…”

Section: Introductionmentioning

confidence: 99%

A Systematic Analysis of Mosquito-Microbiome Biosynthetic Gene Clusters Reveals Antimalarial Siderophores that Reduce Mosquito Reproduction Capacity

Ganley

Pandey

Sylvester³

et al. 2020

Preprint

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21 22 23 24 25 26 2 ABSTRACT 27Advances in infectious disease control strategies through genetic manipulation of insect 28 microbiomes have heightened interest in microbially produced small molecules within 29 mosquitoes. Herein, 33 mosquito-associated bacterial genomes were mined and over 700 30 putative biosynthetic gene clusters (BGCs) were identified, 135 of which belong to known 31 classes of BGCs. After an in-depth analysis of the 135 BGCs, iron-binding siderophores 32 were chosen for further investigation due to their high abundance and well-characterized 33 bioactivities. Through various metabolomic strategies, eight siderophore scaffolds were 34 identified in six strains of mosquito-associated bacteria. Among these, serratiochelin A 35 and pyochelin were found to reduce female Anopheles gambiae overall fecundity likely by 36 lowering their blood feeding rate. Serratiochelin A and pyochelin were further found to 37 inhibit the Plasmodium parasite asexual blood and liver stages in vitro. Our work supplies 38 a bioinformatic resource for future mosquito microbiome studies and highlights an 39 understudied source of bioactive small molecules. 40 41 KEYWORDS 42 Mosquito-Microbiome; Biosynthetic Gene Clusters; Siderophores; Anopheles; 43 Plasmodium 44 45 46 100 transmit avian malaria, P. gallinaceum (Alavi et al., 2003), as well as human infectious 101 agents including dengue virus, Zika virus, and others (2016). As a relevant disease vector,102 Aedes-associated bacteria were also included in our data set. Due to the limited amount 103 of genome sequencing data on mosquito-associated bacteria, especially when compared 104 5 to human microbiomes, our sample size is smaller than analogous studies (Aleti et al., 105 2019; Donia et al., 2014; Helfrich et al., 2018), however this enabled an in-depth 106 bioinformatic analysis. Despite this size, our data set well-represents the unique gut 107 microbiome of mosquitoes. Our previous metagenomic study demonstrated that within the 108 gut microbiota of field-caught A. gambiae mosquitoes four days post blood-meal, 5 109 bacterial genera (Serratia, Elizabethkingia, Acinetobacter, Enterobacter, and 110 Pseudomonas) constitute 84 % of the 16S rRNA reads (Wang et al., 2011), all of which 111 are represented within our sample set. 112 A phylogenetic tree comparing the 33 bacterial strains was generated (Figure 113 1). The best-represented bacterial phylum in our sample set was Proteobacteria (16 γ-114 Proteobacteria, 1 β-Proteobacteria, and 3 α-Proteobacteria), consistent with reports 115 showing Proteobacteria are often the overwhelming microbial phylum in the midgut of 116 field-caught Anopheles mosquitoes (Wang et al., 2011). Species in the Bacteroidetes 117 phylum, which include Elizabethkingia spp., were the second most recurrent group (4 118 Elizabethkingia spp. and 1 Sphingobacterium sp.) in our analysis. Elizabethkingia spp. are 119 well-established mosquito symbionts that are the dominant species within midguts 4-and 120 7-days post-blood meal (PBM) as well as 7-day...

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