Natural products from Photorhabdus and Xenorhabdus: mechanisms and impacts

Çimen, Harun; Touray, Mustapha; Gülşen, Şebnem Hazal; Hazır, Selçuk

doi:10.1007/s00253-022-12023-9

Cited by 29 publications

(36 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is reported that up to 6.5% of the genomes of Xenorhabdus spp. are dedicated to NPs biosynthesis, which is comparable to the well-known NP producers, Streptomyces or myxobacteria. It is worth noting that these NPs, most of which are nonribosomal peptides, polyketides, and/or their hybrid compounds, demonstrate attractive and promising application prospects in the field of plant protection .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Fcl-29 as an Effective Antifungal Natural Product against Fusarium graminearum and Combinatorial Engineering Strategy for Improving Its Yield

Yuan

Duan

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Fusarium head blight (FHB), caused by Fusarium graminearum, whose occurrence and prevalence causes 10–70% wheat production loss, is one of the most destructive diseases influencing the production of wheat globally. To identify the potential natural products (NPs) against F. graminearum, we screened 59 Xenorhabdus strains and discovered that the cell-free supernatant (CFS) of X. budapestensis 14 (XBD14) displays the highest bioactivity. Multiple genetic methods coupled with HRMS/MS analysis determined the major antifungal NP to be Fcl-29, a fabclavine derivative. Fcl-29 was found to effectively control FHB of wheat in the field test and demonstrated broad-spectrum antifungal activity against important pathogenic fungi. The production of Fcl-29 was dramatically improved by 33.82-fold with the combinatorial strategy of genetic engineering (1.66-fold) and fermentation engineering (20.39-fold). The exploration of a new biofungicide in global plant protection is now possible.

show abstract

Section: Introductionmentioning

confidence: 99%

“…In addition, other NPs produced by Xenorhabdus spp. such as benzylideneacetone, indole derivatives, and other bacteriocins were also reported to have antimicrobial capability. ,− …”

Section: Introductionmentioning

confidence: 99%

Identification of Fcl-29 as an Effective Antifungal Natural Product against Fusarium graminearum and Combinatorial Engineering Strategy for Improving Its Yield

Yuan

Duan

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…28 The toxic secondary metabolites produced by EPN or Bt are the main cause of insect death following application, and the detoxifying enzyme in insects plays a key role in the metabolism of these toxins. 21,[29][30][31][32] In insects, the AHR/ARNT, GPCR, HR96, CncC/Keap1, and MAPK/CREB signaling pathways are all involved in the regulation of detoxifying enzyme genes, while only the activation of the CncC/Keap1 pathway is related to the accumulation of ROS. 33 Previous studies have shown that phoxim, pyriproxyfen, or chlorantraniliprole exposure can activate the PI3K/Akt/CncC signaling pathway and increase the transcription of various detoxifying enzymes in Bombyx mori.…”

Section: Introductionmentioning

confidence: 99%

“…The toxic secondary metabolites produced by EPN or Bt are the main cause of insect death following application, and the detoxifying enzyme in insects plays a key role in the metabolism of these toxins 21,29–32 . In insects, the AHR/ARNT, GPCR, HR96, CncC/Keap1, and MAPK/CREB signaling pathways are all involved in the regulation of detoxifying enzyme genes, while only the activation of the CncC/Keap1 pathway is related to the accumulation of ROS 33 .…”

Section: Introductionmentioning

confidence: 99%

PI3K/Akt/CncC signaling pathway mediates the response to EPN‐Bt infection in Holotrichia parallela larvae

ErTao

Wang

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

Background Combining the entomopathogenic nematode (EPN), Heterorhabditis beicherriana LF strain, and Bacillus thuringiensis (Bt) HBF‐18 strain is a practical strategy to manage the larvae of Holotrichia parallela Motschulsky (white grubs). However, the mechanisms underlying the larval defense response to this combined biocontrol strategy are unknown. Results The activities of some antioxidant enzymes (SOD, POD, CAT) and some detoxifying enzymes (AChE, P‐450, CarE, GST) in grubs showed an activation–inhibition trend throughout the EPN‐Bt exposure time course. Eight potentially key antioxidant and detoxifying enzyme genes in response to EPN‐Bt infection were identified from the midgut of grubs through RNA sequencing. After silencing CAT, CarE18, and GSTs1, the enzyme activities were significantly decreased by 30.29%, 68.80%, and 34.63%, respectively. Meanwhile, the mortality of grubs was increased by 18.40%, 46.30%, and 42.59% after exposure to EPN‐Bt for 1 day. Interestingly, the PI3K/Akt signaling pathway was significantly enriched in KEGG enrichment analysis, and the expression levels of phosphatidylinositol 3‐kinase (PI3K), protein kinase B (Akt), cap ‘n’ collar isoform‐C (CncC), kelch‐like ECH‐associated protein 1 (Keap1), and CarE18 were all up‐regulated when exposed to EPN‐Bt for 1 day. Furthermore, RNAi‐mediated PI3K silencing showed a similar down‐regulated trend between PI3K/Akt/CncC and CarE18. Moreover, silencing PI3K rendered grubs more susceptible to EPN‐Bt and accelerated symbiotic bacteria multiplication in grubs. Conclusion These results suggest that the PI3K/Akt/CncC pathway mediates the expression of CarE18 and participates in the defense response of H. parallela larvae against EPN‐Bt infection. Our data provide valuable insights into the design of appropriate management strategies for this well‐known agricultural pest. © 2022 Society of Chemical Industry.

show abstract

“…Rhabdopeptides biosynthesized by NRPSs are very common in Xenorhabdus species, and show cytotoxic and antiprotozoal activities that might be involved in the protection of the insect cadaver (Bozhuyuk et al, 2016; Cai et al, 2017 ; Bi et al, 2018 ). Several Xenorhabdus species have been revealed to contain about twenty biosynthetic gene clusters, most of which are uncharacterized ( Chaston et al, 2011 ; Cimen et al, 2022 ). Therefore, Xenorhabdus spp.…”

Section: Introductionmentioning

confidence: 99%

A novel tumor-targeting strain of Xenorhabdus stockiae exhibits potent biological activities

Zhang

Hanna

Hüttel

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Xenorhabdus are symbionts of soil entomopathogenic nematodes of the genus Steinernema presenting two distinct forms in their life cycle, and can produce a broad range of bioactive compounds. In this study, a novel Xenorhabdus stockiae strain HN_xs01 was isolated from a soil sample via an entrapment method using Galleria melonella nematodes. The supernatants of strain HN_xs01 exhibited antimicrobial properties against Gram-negative and Gram-positive bacteria, and insecticidal properties against Helicoverpa armigera larvae, and antitumor properties as well. Moreover, three linear rhabdopeptides (1, 2 and 3) were identified from strain HN_xs01 using nuclear magnetic resonance analysis, which exhibited significant cytotoxic activity against the human epithelial carcinoma cell line A431 and the human chronic myelogenous leukemia cell line K562. Some bacteria have been reported to colonize the tumor region, and we determined that HN_xs01 could grow in tumor xenografts in this study. HN_xs01 invaded and replicated in B16 melanoma cells grafted into C57BL/6 mice, resulting in tumor inhibition. Moreover, strain HN_xs01 not only merely aggregated in the tumor environment, but also prevented pulmonary metastasis. It caused fragmentation of vessels and cell apoptosis, which contributed to its antitumor effect. In conclusion, X. stockiae HN_xs01 is a novel tumor-targeting strain with potential applications in medicinal and agricultural fields.

show abstract

Natural products from Photorhabdus and Xenorhabdus: mechanisms and impacts

Cited by 29 publications

References 131 publications

Identification of Fcl-29 as an Effective Antifungal Natural Product against Fusarium graminearum and Combinatorial Engineering Strategy for Improving Its Yield

Identification of Fcl-29 as an Effective Antifungal Natural Product against Fusarium graminearum and Combinatorial Engineering Strategy for Improving Its Yield

PI3K/Akt/CncC signaling pathway mediates the response to EPN‐Bt infection in Holotrichia parallela larvae

A novel tumor-targeting strain of Xenorhabdus stockiae exhibits potent biological activities

Contact Info

Product

Resources

About