Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid

Marinho, Rita de Cássia Nunes; Ferreira, Linnajara de Vasconcelos Martins; Silva, Aleksandro Ferreira da; Martins, Lindete Míria Vieira; Nóbrega, Rafaela Simão Abrahão; Fernandes‐Júnior, Paulo Ivan

doi:10.1590/1678-4499.003

Cited by 27 publications

(27 citation statements)

References 24 publications

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“…Bradyrhizobium, Rhizobium and Paraburkholderia genera are well known for their ability to efficiently nodulate legumes in several ecological regions, including the Brazilian Semi-Arid, 16 , 46 , 47 , 55 , 56 as observed in the present study. The plant inoculation experiment showed that the reference strain BR 5609 of B. elkanii , and the isolates ESA 96 and ESA 97, two B. elkanii like from Caruaru, pointed out the nodulation parameters.…”

Section: Discussionsupporting

confidence: 75%

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest

Rodrigues

Silva

Cavalcanti

et al. 2018

Brazilian Journal of Microbiology

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Erythrina velutina (“mulungu”) is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of “mulungu” rhizobia are poorly understood. The aim of this study was to characterize “mulungu” rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic genes. The bacteria showed a high variable metabolic profile. Bradyrhizobium (6), Rhizobium (3) and Paraburkholderia (1) were identified, differing from their geographic origin. The isolates grew up to 45 °C to 0.51 mol L−1 of NaCl. Bacteria which produced more auxin in the medium with l-tryptophan and two Rhizobium and one Bradyrhizobium were phosphate solubilizers. All bacteria nodulated and ESA 90 (Rhizobium sp.) plus ESA 96 (Paraburkholderia sp.) were more efficient symbiotically. Diverse and efficient rhizobia inhabit the soils of Caatinga dry forests, with the bacterial differentiation by the sampling sites.

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Section: Discussionsupporting

confidence: 75%

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest

Rodrigues

Silva

Cavalcanti

et al. 2018

Brazilian Journal of Microbiology

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“…The inoculation of Bradyrhizobium showed different effects on the peanut varieties, both in the physiological and plant growth parameters. This results variation is expected since there is a strong interaction between the plant genotype and the inoculated bacterium, as already observed for tropical legumes such as cowpea (Marinho et al 2014(Marinho et al , 2017Alcântara et al 2014) and peanut (Melo et al 2016).…”

Section: Discussionsupporting

confidence: 69%

Can Bradyrhizobium strains inoculation reduce water deficit effects on peanuts?

Barbosa

Brito

Fernandes

et al. 2018

World J Microbiol Biotechnol

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Drought is one of the environmental factors that most affects peanut cultivation in semi-arid regions, resulting in economic losses to growers. However, growth promoting bacteria are able to reduce water deficit damage in some plant species. In this context, this study aimed to evaluate the interaction of Bradyrhizobium strains reducing water stress effects on peanut genotypes by antioxidant enzymes activities, leaf gas exchanges and vegetative growth, as well as to determine the taxonomic positioning of strain ESA 123. The 16S rRNA gene of ESA 123 was amplified by PCR and sequenced by dideoxy Sanger sequencing method. An experiment was performed in greenhouse with three peanut genotypes (BRS Havana, CNPA 76 AM and 2012-4), two Bradyrhizobium strains (SEMIA 6144 and ESA 123), a mineral source of N and an absolute control (without N) under two water regimes (with and without irrigation). Seeds of peanut were sown and the plants were grown until 30 days after emergence. On the 20th day, the water deficit plants group had their irrigation suspended for 10 days. At in silico analyzes, ESA 123 presented 98.97% similarity with the type strain of B. kavangense. Leaf gas exchange was affected by water deficit; as well as alteration of antioxidant activities and reduction of vegetative growth variables. However, some plants inoculated with SEMIA 6144 and ESA 123 strains presented lower reductions and increment of some evaluated variables, mainly the ones inoculated with the ESA 123 strain, Bradyrhizobium sp. from the semi-arid region of Northeast Brazil. This data suggests beneficial effects of the peanut-Bradyrhizobium interaction in a water stress condition, specially with the ESA 123 strain.

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“…The adaptation of cowpea to harsh climatic conditions and the low specificity of the symbiotic association with rhizobia evidence the technological potential of this crop for drylands. In addition, identifying bacteria that associate with the cowpea is a valuable strategy for semiarid regions because it is the first step in developing innovations such as efficient and well-adapted commercial inoculants (Martins et al 2003;Marinho et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have sought to determine the diversity of native nitrogen-fixing bacteria in Brazilian dry regions (Martins et al 2003;Leite et al 2009;Marinho et al 2017;Santos et al 2017;Rodrigues et al 2018), such as in the Lower Half São Francisco River Valley (Leite et al 2009;Nunes et al 2018). However, these studies did not consider the heterogeneity of the agricultural systems in the region.…”

Section: Introductionmentioning

confidence: 99%

Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil

Sena

Nascimento

Lino

et al. 2020

J Soil Sci Plant Nutr

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The objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of Bradyrhizobium were subjected to the nodC symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical Bradyrhizobium cultural features. A total of 161, out of 251 isolates, showed positive amplification of the nodC gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera, Bradyrhizobium and Microvirga, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential.

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Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid

Cited by 27 publications

References 24 publications

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest

Can Bradyrhizobium strains inoculation reduce water deficit effects on peanuts?

Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil

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